1DJG,1QAS


Conserved Protein Domain Family
EFh_PI-PLCdelta1

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cd16217: EFh_PI-PLCdelta1 
Click on image for an interactive view with Cn3D
EF-hand motif found in phosphoinositide phospholipase C delta 1 (PI-PLC-delta1)
PI-PLC-delta1, also termed 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 (PLCD1), or phospholipase C-III (PLC-III), or phospholipase C-delta-1 (PLC-delta-1), is present in high abundancy in the brain, heart, lung, skeletal muscle and testis. It is activated by high calcium levels generated by other PI-PLC family members, and therefore functions as a calcium amplifier within the cell. PI-PLC-delta1 is required for maintenance of homeostasis in skin and metabolic tissues. Moreover, it is essential in trophoblasts for placental development. Simultaneous loss of PI-PLC-delta1 may cause placental vascular defects, leading to embryonic lethality. PI-PLC-delta1 can be positively or negatively regulated by several binding partners, including p122/Rho GTPase activating protein (RhoGAP), Gha/Transglutaminase II, RalA, and calmodulin. It is involved in Alzheimer's disease and hypertension. Furthermore, PI-PLC-delta1 regulates cell proliferation and cell-cycle progression from G1- to S-phase by control of cyclin E-CDK2 activity and p27 levels. It can be activated by alpha1-adrenoreceptors (AR) in a calcium-dependent manner and may be important for G protein-coupled receptors (GPCR) responses in vascular smooth muscle (VSM). PI-PLC-delta1 may also be involved in noradrenaline (NA)-induced phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis and modulate sustained contraction of mesenteric small arteries. In addition, it inhibits thermogenesis and induces lipid accumulation, and therefore contributes to the development of obesity. PI-PLC-delta1 contains a core set of domains, including an N-terminal pleckstrin homology (PH) domain, four atypical EF-hand motifs, a PLC catalytic core, and a single C-terminal C2 domain. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence. PI-PLC-delta1 can regulate the binding of PH domain to PIP2 in a Ca2+-dependent manner through its functionally important EF-hand domains. In addition, PI-PLC-delta1 possesses a classical leucine-rich nuclear export sequence (NES) located in the EF hand motifs, as well as a nuclear localization signal within its linker region, both of which may be responsible for translocating PI-PLC-delta1 into and out of the cell nucleus.
Statistics
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PSSM-Id: 320047
View PSSM: cd16217
Aligned: 8 rows
Threshold Bit Score: 239.255
Threshold Setting Gi: 167882616
Created: 13-Nov-2014
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 30 residues -Click on image for an interactive view with Cn3D
Feature 1:EF-hand motif [structural motif]
Evidence:
  • None

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ##############################                                                  
1DJG_A        12 WIHSCLRKADKNKDNKMNFKELKDFLKELNiqvddgYARKIFRECDHSQTDSLEDEEIETFYKMLTqraEIDRAFEEAAG 91
gi 41056047  141 WIYSSLRKADKNLDNKMSLKEVKSFLKDINvevhedYAEMLFKECDKSNGGFLEGNEIEHFYKILTkreEIDVIYGEYAK 220
gi 556971989 143 WISICFRKTDKNRDNKMDFEELKKFLKNMNievddtYAKELFKECDRSKSDFLESEEVEEFYKRLTkreEIDTIFNTYAK 222
gi 556972012 165 WVSIRLQKADKNKDNMMTFEELKDFLHDINievedlYAKELFKKYDQSGSNKLKKEEIEEFYKELTkqeEIDNAFIEYSG 244
gi 167882616 164 WIISCLKKADKDKDDKLSQSEMKNFLRSINieveddYAEMLFQQCDKSKSGYLAGVEIQHFYDLLThreEINVIYGQYAK 243
1QAS_A        10 WIHSCLRKADKNKDNKMNFKELKDFLKELNiqvddgYARKIFRECDHSQTDSLEDEEIETFYKMLTqraEIDRAFEEAAG 89
gi 157841242 164 WIYECMRKADKNADNKMSLKELKHFLRQINievndsYAETLFRKCDTSNSGTLEGSEIKLFYNLLTqrdEIDVIYDKYAT 243
gi 705695722  36 WIHTCLRKADKNKDNKMSLKELKDFLKEVNievddyHAKKIFQHCDKSKTEALEDDEIEEFYKILTerkEIDKIFQKYSD 115
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                    
1DJG_A        92 SAETLSVERLVTFLQHQQREEeagpaLALSLIERYEPSETAKAQRQMTKDGFLMYLLSAD 151
gi 41056047  221 TDGLMSANDLLNFLLTEQREEvs-qnDALQLIEKYELDENAKAKQQMTQDGFLMYLHQPE 279
gi 556971989 223 QNGNMSRDNLLNFIKNEQKEVvg-reYVESLIEKYELNETAKNQNLMTQDGFLMYLLSSE 281
gi 556972012 245 KDGTMTEAVLLKFLREKQKEDvg-peYAARLIEKYELNEIAKKDSKMTRDGFLMYLLSPE 303
gi 167882616 244 TTGFMSPGNLVEFLMKEQREKat-vaLAHEIIEKYEPDEHVKAKRLLSKDGFLMYMHHPE 302
1QAS_A        90 SAETLSVERLVTFLQHQQREEeagpaLALSLIERYEPSETAKAQRQMTKDGFLMYLLSAD 149
gi 157841242 244 SNGQMSRNDLLNFIQLEQREPvs-lgYAEELIKKYEVDETAKQDKHMSKDGFLMYLNQEG 302
gi 705695722 116 AEGFMSCQNLVRFLYETQQEEda-vvAAPALIQRYEPNERAKRGNAMTKDGFLMYLLSDD 174

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