Conserved Protein Domain Family
EFh_PI-PLCdelta4

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cd16219: EFh_PI-PLCdelta4 
EF-hand motif found in phosphoinositide phospholipase C delta 4 (PI-PLC-delta4)
PI-PLC-delta4, also termed 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4 (PLCD4), or phospholipase C-delta-4 (PLC-delta-4), is expressed in various tissues with the highest levels detected selectively in the brain, skeletal muscle, testis and kidney. It plays a significant role in cell growth, cell proliferation, tumorigenesis, and in an early stage of fertilization. PI-PLC-delta4 may function as a key enzyme in the regulation of PtdIns(4,5)P2 levels and Ca2+ metabolism in nuclei in response to growth factors, and its expression may be partially regulated by an increase in cytoplasmic Ca2+. Moreover, PI-PLC-delta4 binds glutamate receptor-interacting protein1 (GRIP1) in testis and is required for calcium mobilization essential for the zona pellucida-induced acrosome reaction in sperm. Overexpression or dysregulated expression of PLCdelta4 may initiate oncogenesis in certain tissues through upregulating erbB1/2 expression, extracellular signal-regulated kinase (ERK) signaling pathway, and proliferation in MCF-7 cells. PI-PLC-delta4 contains an N-terminal pleckstrin homology (PH) domain, four atypical EF-hand motifs, a PLC catalytic core domain, and a C-terminal C2 domain. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence. Unlike PI-PLC-delta 1 and 3, a putative nuclear export sequence (NES) located in the EF-hand domain, which may be responsible transporting PI-PLC-delta1 and 3 from the cell nucleus, is not present in PI-PLC-delta4.
Statistics
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PSSM-Id: 320049
View PSSM: cd16219
Aligned: 6 rows
Threshold Bit Score: 272.488
Threshold Setting Gi: 74732863
Created: 13-Nov-2014
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Feature 1:EF-hand motif [structural motif]
Evidence:
  • None

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ##############################                                                  
gi 74732863  138 WLSDWFQRGDKNQDGKMSFQEVQRLLHLMNvemdqeYAFSLFQAADTSQSGTLEGEEFVQFYKALTkraEVQELFESFSA 217
gi 123913506 138 WICDWFQKADKNKDGRMNFKEVQDLLKMMNvdmsehHAFRLFQMADKSESGTLEGEEFVLFYKALTqrdEVLKIFQDFSK 217
gi 642130642 317 WIWDWFQKADKNKDGRMNFKEVRTLLKMMNvdmneeYALHLFTLADKSKSGCLENEEFVQFYKILTerkDMWRVFQDYSR 396
gi 556963931 139 WICDWFQKADKNKDGRMNFKEVRDLLKMMNvdmnehHALRLFNMADRSCSGTLEGDEFVLFYKMLTqrdDVVKIFQEFSK 218
gi 632983166 148 WIRDWFQKADKDKDGRMNFKEVRDLLKMMNvnmnelHALSLFEMADKSRSGTLEGEEFVLFYKALTereDLRKIFSHCSA 227
gi 704154466 139 WIRDWFQKADKNKDGRMNFKEVQRLLKMMNidmnedHALRLFQAADKSESGTLEGEEFVLFYKALTqreEVLSLFQDFSE 218
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1                                                                     
gi 74732863  218 DGQKLTLLEFLDFLQEEQKERdct-seLALELIDRYEPSDSGKLRHVLSMDGFLSYLCSKD 277
gi 123913506 218 DGKKLTLLEFVDFLQQGQLEEent-eeIAMDLIARYEPSDTAKKLHAMSIDGFLVYLCSPE 277
gi 642130642 397 DGQTLTLAELKDFLRQEQQEGers-weHAQELIDRYEPSETAKKQGAMSIDGFQMYLVSPE 456
gi 556963931 219 DGKKLTLLEFVDFLQQEQLETdnt-eeYAMDLIARYEPSETAMRLHAMTINGFLMYLCSLE 278
gi 632983166 228 DGQTFSQREFAEFLTEQQLEPadtameTASALIDRHEPSDTAKQLPALTLNGFLAYLRSPE 288
gi 704154466 219 DGKKLTLLELVDFLQQEQLEDegt-eeLAMELIDKYEPSETARARHVLSADGFLMYLCSSE 278

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