Conserved Protein Domain Family
EFh_CREC_Calumenin_like

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cd16226: EFh_CREC_Calumenin_like 
EF-hand, calcium binding motif, found in calumenin, reticulocalbin-1 (RCN-1), reticulocalbin-3 (RCN-3), and similar proteins
The family corresponds to a group of six EF-hand Ca2+-binding proteins, including calumenin (also known as crocalbin or CBP-50), reticulocalbin-1 (RCN-1), reticulocalbin-3 (RCN-3), and similar proteins. Calumenin is an endo/sarcoplasmic reticulum (ER/SR) resident low-affinity Ca2+-binding protein that contains six EF-hand domains and a C-terminal SR retention signal His-Asp-Glu-Phe (HDEF) tetrapeptide. It functions as a novel regulator of SERCA2, and its expressional changes are tightly coupled with Ca2+-cycling of cardiomyocytes. It is also broadly involved in haemostasis and in the pathophysiology of thrombosis. Moreover, the extracellular calumenin acts as a suppressor of cell migration and tumor metastasis. RCN-1 is an endoplasmic reticulum resident Ca2+-binding protein with a carboxyl-terminal His-Asp-Glu-Leu (HDEL) tetrapeptide signal. It acts as a potential negative regulator of B-RAF activation and can negatively modulate cardiomyocyte hypertrophy by inhibition of the mitogen-activated protein kinase signalling cascade. It also plays a key role in the development of doxorubicin-associated resistance. RCN-3 is a putative six EF-hand Ca2+-binding protein that contains five RXXR (X is any amino acid) motifs and a C-terminal ER retrieval signal HDEL tetrapeptide. The RXXR motif represents the target sequence of subtilisin-like proprotein convertases (SPCs). RCN-3 is specifically bound to the paired basic amino-acid-cleaving enzyme-4 (PACE4) precursor protein and plays an important role in the biosynthesis of PACE4.
Statistics
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PSSM-Id: 320024
View PSSM: cd16226
Aligned: 28 rows
Threshold Bit Score: 296.802
Threshold Setting Gi: 196016914
Created: 24-Nov-2014
Updated: 18-Aug-2016
Structure
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Aligned Rows:
  next features
Feature 1:EF-hand motif [structural motif]
Evidence:
  • None

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #################### #########                                                  
gi 30316268   44 PHDDAHGNFQYDHEAFLGREvAKEFDQLTPeesqaRLGRIVDRMDraGDGDGWVSLAELRAWIAHTQqrhirdSVSAAWD 123
gi 674590833  33 EENPELHNLKFDHEAFLGKElAAEFDKLTDkesreRLTEIFKKID--QDGDKAVSEVELKRWISFVSktavraDANRHWK 110
gi 675871440  39 FENDDEHNAEYDHDAFLGEEeADEFDDLTPeeskeKLAILFDKID--TDGDGLISEDEMQTWIRKIQnqdaskDADKKWD 116
gi 353230743  37 ADDPNRHDIEFDHNAFLGEEtAKEFSQLTPneseeQLKIIIRKID--KNNDERITEIELKSWIEYVAkkskqnSTDRQWN 114
gi 358337392  43 ADDPSRHDVNFDHAAFLGSEtAKEYSKLTPeqsreKLKEIIRKID--KDADGKVTEKEMKEWIAYVAkvgqqqVTEKRWS 120
gi 196016914  10 YDEWGHHNAEFDHRAFIGEE-DDTFHEYSEedasdRLITVFDEID--TNMDEYIDKNEMKARIKGNQlkrlekESREKFK 86
gi 780176664  43 FDEHGKHNPDYDHDAFLGEEeAKKFTNLSPeeskeKLGQLFDRVD--LNKNGSISESELSAWIEIQTnsvlygELDRLFK 120
gi 340374162  39 DPKTNGHNVEYDHEAFLGKEeAEEMQHLSPeeqkrRLQVIFGKID--TNNDKHIEHNELKKWVESVAhrhvidSTAEQMP 116
gi 156368855  38 FDKEGEHNPDYDHEAFLGTE-KEEFDHLPPeeakkRLRALIREVD--NNKDGAVTTEELTDWVKGVFkkrsmeGVDNDLK 114
gi 221091285  29 HYQKGGHNINYDHDAFLGKShGHDFDTLEPseakrRLKIMIKEVD--KNGDGFVSLTELHEWIEYQRksfmreSIDMIID 106
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 30316268  124 TYDTDRDGRVGWEELRNATYGHYapgeefhdve-daetykkmlaRDERRFRVADQDGDSMATREELTAFLHPEEfphm-- 200
gi 674590833 111 NLNANKNEPLAWRDYLERTYGVEdeissdser---haqyikmkrADERRWNAADMNFDNKLSLDEYGAFLHPQDyprm-- 185
gi 675871440 117 EINPKQENSMTWEEYEFFSYGTResidsnpd-----eklhelikKDQKRWRAADFDQDSLLSRSEFSAFMFPESvkem-- 189
gi 353230743 115 DINPTNQPVIKWTEYLMRTYGPEeerlkdtat---sesykkavqHDRRRWIAADLDEDDSLNKTEFTDFVHPEDrpnm-- 189
gi 358337392 121 EVNQQGLNPLPWEVYVEASYGKEedrlkdvet---adayrrvvkQDKRRWDAADLDKDNALTKEEFADFLNPEDkahm-- 195
gi 196016914  87 VLDVNEDSMLPWEEYKQVMFANDlaenngkls----dsmqsmytNDHRKFIDADQDNDGMLTLTEFAAFNFPHNfphm-- 160
gi 780176664 121 AHNMNGDDLLTWAEYNHTTYSGLpleklitmqedktldfrkkvrQDKARWSLADQNRDDALDREEYMAFEWPREkihm-- 198
gi 340374162 117 EFDKNKDGKVTLEEYHSTAYGEVededaeydph-rklsfkemkaRDKRRFDSADKDNDGSLNKEEFGTFLHPEDndhm-- 193
gi 156368855 115 EKDANEDGKVDWNEYSKGTYGDQteddee---------mkeflrRDKRRFDAADTNKDGFLTREEMAIFLHPESspem-- 183
gi 221091285 107 RDDDNKDKQISWKEYKYAHYGKWddeasidk------klrekinNAKHKFNVADEDFDGKLNREEYMMFRHPEEstrvsl 180
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
gi 30316268  201 rdiVIAETLEDLDRNKDGYVQVEEYIADLYSAEpgeeep-----------------awvqtERQQFRDFrDLNKDGHLDG 263
gi 674590833 186 rnvVVEETLDSVDTDKDGYISIDEYLVDLARSFgrqfdpkg------------plddwgqkEKEQFLVHrDKNGDKRMDR 253
gi 675871440 190 edvVAEETLEEIDANKDGLVDLQEFIGEKLFQWllgyandmdiensededteesnaewiesEKQNFIENlDKNKDGKMDR 269
gi 353230743 190 rdaVIDELLEYVDKDNDGYVSEKEYLVDLARAYqstpfdene-----------pepewverERSQFRRFrDTNQDGRMDR 258
gi 358337392 196 rdaVIDELLEAVDTDRNGQVSEREYLDDLARAYqtplvdge------------pepdwvarEREQYQKHrDIDHNGFMDR 263
gi 196016914 161 qnaLAMESLDTYDKNKDGKISWKEYISSMYNSEdnkeqp-----------------gwvrdEEKIYLLRhDKDGDELLDL 223
gi 780176664 199 kdvAIAETIEDIDTDGDGYVNFDEFMKDLWDGQgempd-------------------wveaERKGFAEYrDKDGDGKLNH 259
gi 340374162 194 rdiVIDEAMEDMDKDKDGFIALQEYVDDIWPKNdrqedes--------------epdwvksEREQFSQHrDSNKDGKLDK 259
gi 156368855 184 sevHILETIEDIDRDKDGLISLKEFLGEYEEEPgevpd-------------------wvkdETNRFNEEyDKNKDGKLDK 244
gi 221091285 181 qeiAIDEIIDEMDVNKDRLVDLNEFLGQYVDDRtnppd-------------------wvveDRKHFAKTlDLDGSGKLDR 241
                        250       260       270       280
                 ....*....|....*....|....*....|....*....|....*....
Feature 1                                                         
gi 30316268  264 SEVGHWVLPPAqdq-plvEANHLLHESDTDKDGRLSKAEILGNWNMFVG 311
gi 674590833 254 QEIGEWIMPTDydp-veaEAKHLIYHADDDKDGKLQEKEVLEHQTLFVG 301
gi 675871440 270 EEVKSWLMSEEwnq-vneETDHLFKEADEDMDKHLSKSEMLNKFDLFVG 317
gi 353230743 259 AEVGEWIMPSNydp-idaETKHLFYHADTNKDGLLTEAEIIAKRDTFVS 306
gi 358337392 264 SEVGEWVMPTGydp-ieaETQHLFYHADIDKDDVLTPEEILDKQDLFVS 311
gi 196016914 224 SEIKSWIAPEEnnd-eeeEANHLIESADLDQDGKLTREELLSHQSLFAG 271
gi 780176664 260 EEVGDWIMPTHydp-iesEAKHLMYETDENKDNELTKEEMILHFKLFVG 307
gi 340374162 260 RELGSWIAPDGhdn-aeaEARHLIFNADKDKDGKLTMAEMLENEELFIG 307
gi 156368855 245 EEVRLWILPETdhlmaaeEAKHLVSSADDNSDGKLTIEEIEKNYAVFVG 293
gi 221091285 242 NEMRNWVLPKLset--keEANHLIKGADDNNDNKLSYEEILDHYNLFVG 288

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