1NUB,1SRA,1BMO,2V53


Conserved Protein Domain Family
EFh_SPARC_like

?
cd16231: EFh_SPARC_like 
Click on image for an interactive view with Cn3D
EF-hand, extracellular calcium-binding (EC) motif, found in secreted protein acidic and rich in cysteine (SPARC) and similar proteins
This family includes secreted protein acidic and rich in cysteine (SPARC), secreted protein, acidic and rich in cysteine-like 1 (SPARCL1), and similar proteins. SPARC is a prototypic collagen-binding matricellular protein that is involved in extracellular matrix (ECM) assembly and fibrosis through binding both fibrillar collagen and basal lamina collagen IV. It regulates the activity of matrix metalloproteinases (MMPs), as well as the growth factor signaling mediated by cell surface receptors including vascular endothelial growth factor (VEGF) receptor, basic fibroblast growth factor (bFGF), and transforming growth factor (TGF) beta1. It also shows survival activity in tumor progression. SPARC contains an N-terminal acidic 52-residue segment followed by a follistatin-like (FS) domain, and an alpha-helical EC domain with 2 unusual calcium-binding EF-hands and the collagen-binding site. SPARCL1 is the closest family member to SPARC. It shares the three primary domains contained within SPARC with an expanded N-terminal domain. SPARCL1 may function as both a tumor suppressor and as a regulator of angiogenesis. It can bind to collagens and be counter-adhesive to wild-type dermal fibroblasts, but do not influence rates of cell proliferation. Moreover, SPARCL1 can influence central nervous system (CNS) development and synaptic rearrangement.
Statistics
?
PSSM-Id: 320010
View PSSM: cd16231
Aligned: 14 rows
Threshold Bit Score: 175.231
Threshold Setting Gi: 229299067
Created: 18-Nov-2014
Updated: 18-Aug-2016
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:Ca binding site [ion binding site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                                                        
1NUB_A        89 CLDSELTEFPLRMRDWLKNVLVTLYerdednnlltekqklrvkki---------henekrleagdhpeknynmYIFPVHW 159
1SRA_A         3 CLDSELTEFPLRMRDWLKNVLVTLYerdednnlltekqklrvkkihene-krleagdhpvellardfeknynmYIFPVHW 81
2V53_A        90 CLDSELTEFPLRMRDWLKNVLVTLYerdednnlltekqklrvkki---------henekrleagdhpeknynmYIFPVHW 160
gi 259016170 515 CTDFEVIQFPLRMRDWLKNILMQLYeansehagylnekqrnkvkkiyldekrllagdhpidlllrdfkknyhmYVYPVHW 594
gi 46561990  216 CGEHELSEYPTRMRSWIKNIYLQMYdeaedmgglnekqrfhgrklvanrdrlqtyeehhidlmsrefqkfyplYKYPIHW 295
gi 229299067  87 CLEEELAEFPLRMRDWLKNVLVQRMslelltdkemfhakkiyederrlk-kradggdweaeillrdfrknyahYVYPIHW 165
gi 464320    141 CTEEHMAQFPERMADWLFQVMKELKkrrelhklewee------------------------llseaenddekkHVYPVIW 196
gi 117371494 151 CSAEDLREYPERMTNWFIKSLALIRnrpeehgglsqk-------------------------eketlqqewsqSTHAIFW 205
gi 11995033  158 CSEQEMDDFPRRMRDWLFNIMRDLAarhelsphylkl-------------------------ekeaeveqskrWANAAIW 212
gi 17946659  171 CEGEDLKDFPRRMRDWLFYVMRDLAerdeltehymqm-------------------------eleaetnnsrrWSNAAVW 225
                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
Feature 1             #    #      #                      # # #      #         
1NUB_A       160 QFGQLDqhPIDGYLSHTELAPLRAPLIpMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQ 220
1SRA_A        82 QFGQLDqhPIDGYLSHTELAPLRAPLIpMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQ 142
2V53_A       161 QFGQLDqhPIDGYLSHTELAPLRAPLIpMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQ 221
gi 259016170 595 QFSELDqhPMDRVLTHSELAPLRASLVpMEHCITRFFEECDPNKDKHITLKEWGHCFGIKE 655
gi 46561990  296 KFGILDvnPTDKYLSKRELEPMRAPLVpLEHCTDTFFSTADANKDHLISLYEWAEALGLQE 356
gi 229299067 166 MFSKLDihPKDRYLTHAELAPLRAPLVpMEHCTTTFFKTCDDDGDKRISLYEWGACLELKE 226
gi 464320    197 KFCELDtkPHDKSVSHHELIPITAPVIpMESCIKPFLEGCDANNDGNISIKEWGKCLGLKE 257
gi 117371494 206 KFDRLDknPSDHYLDFAELEGLRAPIVlFEPCTKPFLQACDVDNDTLISAVEFGRCLNLSD 266
gi 11995033  213 KFCDLDghPHDRKVSRHELFPIKAPLMaLEHCISPFLNKCDVDDDHFITLREWGKCLEIPE 273
gi 17946659  226 KWCDLDg-DTDRSVSRHELFPIRAPLVsLEHCIAPFLESCDSNKDHRITLVEWGACLELDP 285

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap