2ZF3,3BMZ,2ZF4


Conserved Protein Domain Family
LolA_VioE

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cd16330: LolA_VioE 
Click on image for an interactive view with Cn3D
Proteins similar to violacein biosynthetic enzyme VioE that shares fold with periplasmic molecular chaperone LolA
This family includes the violacein biosynthetic enzyme VioE which shares a core fold with lipoprotein transporter proteins that include lipoprotein transporter proteins LolA and LolB. VioE is an enzyme with no characterized homologs that plays a key role in the biosynthesis of violacein, a naturally occurring bisindole product with various biological activities, including antitumor activity as well as antibacterial and cytotoxic properties. In Chromobacterium violaceum, VioE catalyzes the third step in violacein biosynthesis from a pair of Trp residues (i.e. mediates a 1,2 shift of an indole ring and oxidative chemistry) to generate prodeoxyviolacein, a precursor to violacein. Structural and mutagenesis studies suggest that VioE acts as a catalytic chaperone, using this fold associated with lipoprotein transporters to catalyze the production of its prodeoxyviolacein product.
Statistics
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PSSM-Id: 319987
Aligned: 7 rows
Threshold Bit Score: 276.29
Created: 8-Oct-2015
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
 
homodimerhydrophobic
Conserved site includes 27 residues -Click on image for an interactive view with Cn3D
Feature 1:homodimer interface [polypeptide binding site]
Evidence:
  • Structure:3BMZ: Chromobacterium violaceum violacein biosynthetic enzyme VioE dimer interface; contacts at 4.0A
    View structure with Cn3D
  • Structure:2ZF4: Chromobacterium violaceum violacein biosynthetic enzyme VioE dimer interface; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 # # # ##      ### # #        # ###           # #              # # # 
2ZF3_A      8 EPPLLp-aRWSSAYVSYWSPmlpdDQLTSGYCWFDYeRDICRIDGLFNPWserDTGYRLWMSEVGnaasgRTWKQKVAYg 86  Chromobacterium vi...
3BMZ_A      5 EPPLLp-aRWSSAYVSYWSPmlpdDQLTSGYCWFDYeRDICRIDGLFNPWserDTGYRLWMSEVGnaasgRTWKQKVAYg 83  Chromobacterium vi...
2ZF4_A      8 EPPLLp-aRWSSAYVSYWSPmlpdDQLTSGYCWFDYeRDICRIDGLFNPWserDTGYRLWMSEVGnaasgRTWKQKVAYg 86  Chromobacterium vi...
AFT64084    9 KPPKLp-aQWSSSYISYWQPmqatDHITSGYCWFDYeRNVCRIDGLFNPWsetETGHRLWMSEVMqpnscESFKAKIAYa 87  gamma proteobacter...
AGC43167    6 LPPLLp-lQWSSGYVSYWSPmrgdDQLTSGYCWFDYaREVCRIDGLFNPWpekEKGYQLWMSEVGdvtrgQSLQKKVAY- 83  Myxococcus stipita...
AFY56407  382 TPPLLp-kQWSTSYVSYWQPmldeDQITSGYCWFDYsNNLGRIDGLFNPWsekETGHRLWMSQIFtpgkgETRMSKVAYa 460 Rivularia sp. PCC ...
AHE14571    7 RPPLLperQWSSSYVSYWSPmqggDRMTSGHVWYDYgRGVYRIDGVLNPWdeqAHGHLLWKSEINqfgagECHTWAVAYd 86  uncultured bacterium
Feature 1             ### # #                                                              #  
2ZF3_A     87 RERTALg-EQLCERPLDDETGPfaelFLPRDVLRRlGARHIGRRvvlgreADGWRYQRPg-KGPSTLYLDaasgtPLRMV 164 Chromobacterium vi...
3BMZ_A     84 RERTALg-EQLCERPLDDETGPfaelFLPRDVLRRlGARHIGRRvvlgreADGWRYQRPg-KGPSTLYLDaasgtPLRMV 161 Chromobacterium vi...
2ZF4_A     87 RERTALg-EQLCERPLDDETGPfaelFLPRDVLRRlGARHIGRRvvlgreADGWRYQRPg-KGPSTLYLDaasgtPLRMV 164 Chromobacterium vi...
AFT64084   88 REHMDRt-SQFEAVVLDDEVDPchnlILMQDVLEEcDARFVGRQevlgqdAEVWTFERPegKGPATYYFAsgtnqLLRMV 166 gamma proteobacter...
AGC43167   84 RRGTPPsgDGLFADVLPEELSPfqelFLPRAVLVDgHARHSGWAfvlghrADIWTLDRPr-KTPLRFYLQagtnvLLRMV 162 Myxococcus stipita...
AFY56407  461 RKENEQ--TAYKAVNLEDDVDSchelLLTQDILIRhNATYAGSHtilgikTDAWKFKRPg-KAVSTYYFKqgtnhLIRMV 537 Rivularia sp. PCC ...
AHE14571   87 VPGEAA--PTYRARPLAYERTKspgsLVPCDVLVRhEAVFVGTEqvlgfeAEGWRYERPegRGPVTYYFRagtrhLLRMV 164 uncultured bacterium
Feature 1                #  #   
2ZF3_A    165 TGDeasRASLRDFpNVSE 182 Chromobacterium violaceum
3BMZ_A    162 TGDeasRASLRDFpNVSE 179 Chromobacterium violaceum ATCC 12472
2ZF4_A    165 TGDeasRASLRDFpNVSE 182 Chromobacterium violaceum
AFT64084  167 TGDpntMASIRDFpNFNT 184 gamma proteobacterium D250
AGC43167  163 SGNdpqHVSVRDFpNLSI 180 Myxococcus stipitatus DSM 14675
AFY56407  538 TGDpnkHASVRDFpNLNT 555 Rivularia sp. PCC 7116
AHE14571  165 NGDprlHAAIRDFpTFSE 182 uncultured bacterium

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