2ZF3,3BMZ,2ZF4


Conserved Protein Domain Family
LolA_VioE

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cd16330: LolA_VioE 
Click on image for an interactive view with Cn3D
Proteins similar to violacein biosynthetic enzyme VioE that shares fold with periplasmic molecular chaperone LolA
This family includes the violacein biosynthetic enzyme VioE which shares a core fold with lipoprotein transporter proteins that include lipoprotein transporter proteins LolA and LolB. VioE is an enzyme with no characterized homologs that plays a key role in the biosynthesis of violacein, a naturally occurring bisindole product with various biological activities, including antitumor activity as well as antibacterial and cytotoxic properties. In Chromobacterium violaceum, VioE catalyzes the third step in violacein biosynthesis from a pair of Trp residues (i.e. mediates a 1,2 shift of an indole ring and oxidative chemistry) to generate prodeoxyviolacein, a precursor to violacein. Structural and mutagenesis studies suggest that VioE acts as a catalytic chaperone, using this fold associated with lipoprotein transporters to catalyze the production of its prodeoxyviolacein product.
Statistics
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PSSM-Id: 319987
View PSSM: cd16330
Aligned: 7 rows
Threshold Bit Score: 276.29
Threshold Setting Gi: 568605197
Created: 8-Oct-2015
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
homodimerhydrophobic
Conserved site includes 27 residues -Click on image for an interactive view with Cn3D
Feature 1:homodimer interface [polypeptide binding site]
Evidence:
  • Structure:3BMZ: Chromobacterium violaceum violacein biosynthetic enzyme VioE dimer interface; contacts at 4.0A
    View structure with Cn3D
  • Structure:2ZF4: Chromobacterium violaceum violacein biosynthetic enzyme VioE dimer interface; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    # # # ##      ### # #        # ###           # #              # # # 
2ZF3_A         8 EPPLLp-aRWSSAYVSYWSPmlpdDQLTSGYCWFDYeRDICRIDGLFNPWserDTGYRLWMSEVGnaasgRTWKQKVAYg 86
3BMZ_A         5 EPPLLp-aRWSSAYVSYWSPmlpdDQLTSGYCWFDYeRDICRIDGLFNPWserDTGYRLWMSEVGnaasgRTWKQKVAYg 83
2ZF4_A         8 EPPLLp-aRWSSAYVSYWSPmlpdDQLTSGYCWFDYeRDICRIDGLFNPWserDTGYRLWMSEVGnaasgRTWKQKVAYg 86
gi 407188288   9 KPPKLp-aQWSSSYISYWQPmqatDHITSGYCWFDYeRNVCRIDGLFNPWsetETGHRLWMSEVMqpnscESFKAKIAYa 87
gi 441486472   6 LPPLLp-lQWSSGYVSYWSPmrgdDQLTSGYCWFDYaREVCRIDGLFNPWpekEKGYQLWMSEVGdvtrgQSLQKKVAY- 83
gi 427372451 382 TPPLLp-kQWSTSYVSYWQPmldeDQITSGYCWFDYsNNLGRIDGLFNPWsekETGHRLWMSQIFtpgkgETRMSKVAYa 460
gi 568605197   7 RPPLLperQWSSSYVSYWSPmqggDRMTSGHVWYDYgRGVYRIDGVLNPWdeqAHGHLLWKSEINqfgagECHTWAVAYd 86
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                ### # #                                                              #  
2ZF3_A        87 RERTALg-EQLCERPLDDETGPfaelFLPRDVLRRlGARHIGRRvvlgreADGWRYQRPg-KGPSTLYLDaasgtPLRMV 164
3BMZ_A        84 RERTALg-EQLCERPLDDETGPfaelFLPRDVLRRlGARHIGRRvvlgreADGWRYQRPg-KGPSTLYLDaasgtPLRMV 161
2ZF4_A        87 RERTALg-EQLCERPLDDETGPfaelFLPRDVLRRlGARHIGRRvvlgreADGWRYQRPg-KGPSTLYLDaasgtPLRMV 164
gi 407188288  88 REHMDRt-SQFEAVVLDDEVDPchnlILMQDVLEEcDARFVGRQevlgqdAEVWTFERPegKGPATYYFAsgtnqLLRMV 166
gi 441486472  84 RRGTPPsgDGLFADVLPEELSPfqelFLPRAVLVDgHARHSGWAfvlghrADIWTLDRPr-KTPLRFYLQagtnvLLRMV 162
gi 427372451 461 RKENEQ--TAYKAVNLEDDVDSchelLLTQDILIRhNATYAGSHtilgikTDAWKFKRPg-KAVSTYYFKqgtnhLIRMV 537
gi 568605197  87 VPGEAA--PTYRARPLAYERTKspgsLVPCDVLVRhEAVFVGTEqvlgfeAEGWRYERPegRGPVTYYFRagtrhLLRMV 164
                        170
                 ....*....|....*...
Feature 1                   #  #   
2ZF3_A       165 TGDeasRASLRDFpNVSE 182
3BMZ_A       162 TGDeasRASLRDFpNVSE 179
2ZF4_A       165 TGDeasRASLRDFpNVSE 182
gi 407188288 167 TGDpntMASIRDFpNFNT 184
gi 441486472 163 SGNdpqHVSVRDFpNLSI 180
gi 427372451 538 TGDpnkHASVRDFpNLNT 555
gi 568605197 165 NGDprlHAAIRDFpTFSE 182

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