2BYO,4QA8,3MH9,3MH8


Conserved Protein Domain Family
LppX-like

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cd16334: LppX-like 
Click on image for an interactive view with Cn3D
family includes lipoproteins LppX, LprA, LprF and LprG from Mycobacterium tuberculosis
This family includes the homologous lipoproteins LppX, LprA, LprF and LprG from Mycobacterium tuberculosis (Mtb), all of which share a core fold with lipoprotein transporter proteins LolA and LolB. Mtb contains components such as glycolipids, lipoglycans and lipoproteins that play critical roles in regulating host responses and promoting survival of the pathogen. Mtb LprA is a lipoprotein agonist of Toll-like receptor 2 (TLR2) that regulates innate immunity and APC function. LprF, which is also found in Mycobacterium bovis but not in the nonpathogenic Mycobacterium smegmatis, has a central hydrophobic cavity that binds a diacylated glycolipid that it transfers from the plasma membrane to the cell wall, which might be related to the pathogenesis of the bacteria. Similarly, LprG functions as a carrier of glycolipids and lipoglycans, such as lipoarabinomannan (LAM), during their trafficking and delivery to the mycobacterial cell wall, contributing to virulence; LAM inhibits fusion of phagosomes with lysosomes as a means for mycobacteria to evade host defense. In addition, LprG has potent TLR2 agonist activity that modulates antigen processing of dendritic cells and macrophages. LppX is required for the translocation of the key virulence factors, the phthiocerol dimycocerosates (PDIMs), to the surface of Mtb.
Statistics
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PSSM-Id: 319988
View PSSM: cd16334
Aligned: 99 rows
Threshold Bit Score: 104.224
Threshold Setting Gi: 934113069
Created: 13-Oct-2015
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
hydrophobic
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:hydrophobic core
Evidence:
  • Comment:based on Escherichia Coli LolA, LolB and Rseb, Mycobacterium bovis LprF, Chromobacterium violaceum VioE, and Mycobacterium tuberculosis LppX with structures
  • Comment:this surface has an overall hydrophobic character
  • Structure:2BYO: Mycobacterium tuberculosis LppX binds fatty acids in the hydrophobic core; contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                 # #       # #                 #      # #        # #    
2BYO_A        17 PTASDPALLAEIRQSLDATK-GLTSVHV------AVRTTGkvd-----slLGITSA-----DVDVRAn--PLAAKGVCTY 77
gi 934113069  19 CGNADVDAAKVASEGATAFE-SVNNFHF------KLTVSDge-------aAPLGDIiiidaDGDSIRp-dKIQAKIKAKL 83
gi 652461070  25 SGGSEESAPDLLAHAEHILD-ETKTAHF------VLESSGap------dtGTVLTGg----EGDIAR---PASFQGTLKV 84
gi 919593238  17 GGSSAPDARQVLAASQAAMQ-KVHSYHF------NLQVEHags----satLVIQSA-----DGDLVVpdrLQANAGALVL 80
gi 931515153  38 PSPTPLSSVTILESALATME-EVESYHFemvlqmVISSEGt--------tMEVPLTf----IGYFKA---PDRLRGELTM 101
gi 269786974  28 EPTPTPSPQGLIDAAANRFN-ELDTAHY------TLTIDGdvyld-aqgmLALRGA-----EGDLQR---PDRATAKADI 91
gi 148569150  28 TPTPTPTPAEISARAGQATG-AAQSLAF------SITITGqpvytdpsrvFVITSI-----DGALRR---PDGVLATLKL 92
gi 700173447  23 SGGGEPEANADPKAALVAAKtQFDQAKFv-----GLTLSSkdvp---kteNGVTGAi---gTGEISAt--EPKFKGNVTA 89
gi 308227028  35 TPVPTPTPRELGVAIGQATQ-AAESAHF------TIDLSGkpim---vsaFTLTRI-----EGDLKR---PDSVLAILTV 96
gi 219542275  27 TPTPTPDPRTLAAAIGRATQ-NSQSAHF------RITLSGkpva---ldsGGVTVLns--iEGDLRR---PDAVLSILNI 91
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                  # #    # #                                                            
2BYO_A        78 n---dEQGVPFRVQGDNISVKLfd----dWSNLGSI---SELSTSRVLdpaAGVTQLLSGv-TNLQAQGTEVid----gi 142
gi 934113069  84 agapiAVNINGIIIGADAWITPnpfnattYEKLADTgglETFSPAKGI---SDVLRGLKN--PSFVEEAEIDg------v 152
gi 652461070  85 rtsgsPLDLKVISVNGTVYAQLpft--ttYSVVDPAq-fGFGDPGALLdpdTGISQLLAQa-ESAKLGGERRvggevvre 160
gi 919593238  81 g---sLVQVKIIVVGGQQYITDp-----vTGRWQTIn--GLPDPRVLSdsrTGISALLGQi-RNPSTPSESSvg----gv 145
gi 931515153 102 ellgqMMETELISIGEISYIKDpts--geWQMSTESa--TPFTPEDFV---GLEPDDVANm-VDLILIGEENld----gv 169
gi 269786974  92 gfagtTLSVNVVALGQEQYMTNflt--grWERAPED---LDYNPAVVFdseRGIPGVLREt-RDVTLVGEESld----gt 161
gi 148569150  93 rsaagLAEVRTVSLAGKQYATNplt--raWQCLTPG---QAFDAAVLFdpqKGIEQLLQSgiETITLVGEETld----gr 163
gi 700173447  90 tingvTGAAEVITIGDTAYMKFftp-gynETDLGSL---GAPNPSDFFdpaKGISQLLVDt-TDPKAGSEVRdg----aa 160
gi 308227028  97 atgstVAEIRTASVAGTQYMTNplt--reWGCLAEG---DGFNPAVLFdpqVGIEQLLQNgfDEVTLVGTEDln----gv 167
gi 219542275  92 tlgnaIGEIRTISLAGKQYTTNpit--rqWMCLQSG---STFDPATLFapeSGIEALLANklADVTLVGIEDln----gr 162
                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
Feature 1              #                      # #           #  # #             # # #      #   
2BYO_A       143 STTKITGTIpaSSVKMLDPga---ksARPATVWIaq--dgSHHLVRASIDLg-------sGSIQLTQS----KWNEP 203
gi 934113069 153 ATYHISGEVdaQSVSALTGgva-eagTIKLDLWIg----kDDKLLRQLVAVgrlv-ssekETIKRTLVv--sQFNQS 221
gi 652461070 161 VTARIPGELveQILTSKDPs-----kPVEARFSIap---eSGELRRAELTGpfye-agsdATFTVRLS----DFGDD 224
gi 919593238 146 ACWQISGKLdaSYLRGLLGdqtragsQLDAVVWIgk---sDHLLYQLRLTGvaadgdtnqTVRTLKLS----KFNEA 215
gi 931515153 170 PVYHLEGKVptESMGNVLGev---dgEIKVEYWIgv---eDDLIRRVDVEMelfevggdqQELNATLSflfsEFNKE 240
gi 269786974 162 AAYHLRGVVpqASVAPMTGnaf-qgdPIEFDLWIgr---dTSDILKVVLHDtaaaqgatpATWTLVLS----RHNEP 230
gi 148569150 164 LTYHLRGTIpgARMADISGgli-gaaPVTADIWAdr---eTLRLAKIVLVDqapg-apepTTWTLVFS----TYDQP 231
gi 700173447 161 VLQQYSGTLpgSRIKELFNlgd-gtgSFEVTYGIa----eGDKLQLATMKGpffa--gatSTFTLRLK----DYGKS 226
gi 308227028 168 ASYHLRGSMsgEKLLPISMgml-gtgPVTVDLWAdqatmrATKMVLVDTASdat----npTTWTLTFS----DYGKM 235
gi 219542275 163 PHYHLRGTLpaEPLRAISLnll-gvgPVTADMWAdqatmrASRVVLVDTATdat----npSTWTLEFS----DYDKT 230

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