1F9Z,5D7Z,2C21,4MTQ,5D7Z


Conserved Protein Domain Family
GlxI_Ni

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cd16358: GlxI_Ni 
Click on image for an interactive view with Cn3D
Glyoxalase I that uses Ni(++) as cofactor
This family includes Escherichia coil and other prokaryotic glyoxalase I that uses nickel as cofactor. Glyoxalase I (also known as lactoylglutathione lyase; EC 4.4.1.5) is part of the glyoxalase system, a two-step system for detoxifying methylglyoxal, a side product of glycolysis. This system is responsible for the conversion of reactive, acyclic alpha-oxoaldehydes into the corresponding alpha-hydroxyacids and involves 2 enzymes, glyoxalase I and II. Glyoxalase I catalyses an intramolecular redox reaction of the hemithioacetal (formed from methylglyoxal and glutathione) to form the thioester, S-D-lactoylglutathione. This reaction involves the transfer of two hydrogen atoms from C1 to C2 of the methylglyoxal, and proceeds via an ene-diol intermediate. Glyoxalase I has a requirement for bound metal ions for catalysis. Eukaryotic glyoxalase I prefers the divalent cation zinc as cofactor, whereas Escherichia coil and other prokaryotic glyoxalase I uses nickel. However, eukaryotic Trypanosomatid parasites also use nickel as a cofactor, which could possibly be explained by acquiring their GLOI gene by horizontal gene transfer. Human glyoxalase I is a two-domain enzyme and it has the structure of a domain-swapped dimer with two active sites located at the dimer interface. In yeast, in various plants, insects and Plasmodia, glyoxalase I is four-domain, possibly the result of a further gene duplication and an additional gene fusing event.
Statistics
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PSSM-Id: 319965
View PSSM: cd16358
Aligned: 10 rows
Threshold Bit Score: 200.7
Threshold Setting Gi: 90108946
Created: 7-Jan-2016
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Citation:PMID 9218781
  • Comment:There are two active sites between the dimer interface.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #   #                        #    # #              # # #             #        
1F9Z_A         3 LLHTMLRVGDLQRSIDFYTKvLGMKLLRTSENPeyKYSLAFVGYgpeteeAVIELTYNWGvdKYELGtAYGHIALSVDNA 82
5D7Z_A       155 LCQVMLRVGDLERSIKFYEKaLGMKLLRKKDVPdyKYTIAMLGYadedktTVLELTYNYGvtEYSKGnAYAQVAIGTNDV 234
2C21_A         9 MLHTMIRVGDLDRSIKFYTErLGMKVLRKWDVPedKYTLVFLGYgpemssTVLELTYNYGvtSYKHDeAYGHIAIGVEDV 88
4MTQ_A         3 ILHSMLRVADLEAALEFYTRaLDMRLLRRRDYPegRFTLAFVGYqderaaAALELTHNWDrdGYTQGdGYGHLAIEVEDA 82
5D7Z_A        25 MLHAVYRVGDLDRTIKYYTEcFGMKLLRKRDVPdeKYTNAFLGFgpentnFAVELTYNYGvdKYDIGtGFGHFAIANDDV 104
gi 2494847     3 LLHTMIRVGDLDKSLQFYCDiLGMNLLRKKDYPsgEFTLAFVGYgkesenAVIELTHNWGtdKYDLGnGFGHIALGVEDI 82
gi 60392612    3 LLHTMLRVGNLEKSLDFYQNvLGMKLLRRKDYPegRFTLAFVGYgdetdsTVLELTHNWDteRYDLGnAYGHIAVEVDDA 82
gi 66802462    4 ILHTMLRVGNLEKSLQFYIDvLGMKLLRKSENVeyKYTLAFVGYtnedenAVIELTYNWGveKYELGtAFGHIAIGVDDV 83
gi 144898247   5 MLHTMIRVGNLDASIDFYTRlLGMKLLRRQDYPdgRFTLAFVGYgdeadnTVIELTHNWDtaSYDLGtGFGHVALGVPDI 84
gi 168025026   1 MLHVVYRVGDLDKTIKFYTEcLGMKLLRRRDIPeeKYTNAFLGYgpedtnFVVELTYNYGvdKYDIGtGFGHFGIAVEDV 80
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*.
Feature 1                           #           # #       # #  
1F9Z_A        83 AEACEKIRQn----gGNVTReagpvkggttvIAFVEDPDGYKIELI 124
5D7Z_A       235 YKSAEAVDLatkelgGKILRqpgplpgintkIASFVDPDGWKVVLV 280
2C21_A        89 KELVADMRKh----dVPIDYede-----sgfMAFVVDPDGYYIELL 125
4MTQ_A        83 AVTCARARAl----gYRVTReaglmqhgrsvIAFLEDPDGYKVELI 124
5D7Z_A       105 YKLAENIKSk----gGKITRepgpvkggstvIAFAQDPDGYMFELI 146
gi 2494847    83 YSTCDKIRDk----gGKVVRepgpmkhgttvIAFVEDPDGYKIELI 124
gi 60392612   83 YEACERVKRq----gGNVVReagpmkhgttvIAFVEDPDGYKIEFI 124
gi 66802462   84 YETVERIRKs----gGKVAReaapvlggttvIAFVEDPDNYKIELI 125
gi 144898247  85 HATCVQLAQa----gAKITRppgpmkhgstiIAFIEDPDGYKIELI 126
gi 168025026  81 QKVVDLVKAk----gGKVTRepgpvkggksiIAFVEDPDGYKFELI 122

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