1IMQ,4QKO,4UHP,2GZF,1BXI,3GKL,1CEI,1GXH


Conserved Protein Domain Family
Col_Im_like

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cd16363: Col_Im_like 
Click on image for an interactive view with Cn3D
inhibitory immunity (Im) protein of colicin (Col) deoxyribonuclease (DNase) and pyocins
This family contains inhibitory immunity (Im) proteins that bind to colicin endonucleases (DNases) or pyocins with very high affinity and specificity; this is critical for the neutralization of endogenous DNase catalytic activity and for protection against exogenous DNase bacteriocins. The DNase colicin family (ColE2, ColE7, ColE8 and ColE9) in E. coli, and pyocin family (S1, S2, S3 and AP41) in P. aeruginosa, are potent bacteriocins where the immunity proteins (Ims) protect the colicin/pyocin producing (i.e. colicinogenic) bacteria by binding and inactivating colicin nucleases. The binding affinities between cognate and non-cognate nucleases by Im proteins can vary up to 10 orders of magnitude.
Statistics
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PSSM-Id: 319897
View PSSM: cd16363
Aligned: 105 rows
Threshold Bit Score: 66.7664
Threshold Setting Gi: 836694450
Created: 28-Mar-2013
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
heterodimer
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:heterodimer interface [polypeptide binding site]
Evidence:
  • Structure:1BXI: Escherichia coli immunity protein IM9 in complex with colicin E9 DNase domain; contacts at 4.0A
    View structure with Cn3D
  • Structure:4UHP: Pseudomonas aeruginosa immunity protein ImAP41 in complex with pyocin AP41 DNase; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                          ##          #  ##  ##  #      #### ####     #                
1IMQ_A        4 KHSiSDYTEAEFLQLVTTICNadt-----ssEEELVKLVTHFEEmTEHPSGSDLIYYPkegdDDSPSGIVNTVKQWRAAn 78
1BXI_A        4 KASiSDYTEAEFLQLVTTICNadt-----ssEEELVKLVTHFEEmTEHPSGSDLIYYPkegdDDSPSGIVNTVQQWRAAn 78
gi 972247783  4 KNSiNEYSEDEFLRLLQKICKada-----ssESELDALVDHFEDiTEHPDGSDLIYYPddlaDATPERIVEIVKQWRMSq 78
gi 953823826  2 KDRiEEYTEAEFKKLVQDICSvnv-----asEDEHTQLVRLFCRlTQHPDGSDLIYYPddeaDATPERITEIVKNWRADq 76
gi 636573942  9 KDTlSEYTRSDFIELLARICRada-----stEAELNKFVDHFEKiAQHPDGSDLIDYPessaDGTPERITEIVQQWRQSq 83
gi 972247813  2 EKNiTSYTQDEFLSLVRRISIddy-----ptEQEHIDAVLLFEKlTEHPDGSDLFYYPddpaDATPERIVEIVKQWRMSq 76
gi 428764268  4 KQKlEEYTEAEFVQLVGEFFEnrsglhgkafIDYQDYLSDEFERlTEHPAKSDLIYYPeeghSVTPQSIVNHVKEWRAAn 83
gi 321166893  4 KDKfEEYSEAEFVALVKDIFDvh------gsESYQDSLLENFSRvTGHPSGTDLIFYPegdnPVTPESVVTEIKQWRTSv 77
gi 945305640  2 ANEtPGYTEKYFSGLLERIFAanrn---gtpEVLLADMLDNFCElAEHPAGTDLIYWPedetQCTPESITATVKKWRTDh 78
gi 738250038  3 SIRiEDMTRQEFLGLLKRIFDvt------dcEKVHDELIDQFEElSQHPDGSDLIFYPenpeDSTPERIAEIVEEWRARn 76

                ....*.
Feature 1             
1IMQ_A       79 GKSGFK 84
1BXI_A       79 GKSGFK 84
gi 972247783 79 GLPCFK 84
gi 953823826 77 GLPGFK 82
gi 636573942 84 GLPCFK 89
gi 972247813 77 GLPCFK 82
gi 428764268 84 GKPGFK 89
gi 321166893 78 GKTLFR 83
gi 945305640 79 GPTEFK 84
gi 738250038 77 GLPGFN 82

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