3JQO,2BHV,3ZBI


Conserved Protein Domain Family
VirB10_like

?
cd16426: VirB10_like 
Click on image for an interactive view with Cn3D
VirB10 and similar proteins form part of core complex in Type IV secretion system (T4SS)
This family contains VirB10, a component of the type IV secretion system (T4SS) and its homologs, including TraB, TraF, IcmE, and similar proteins. T4S system is employed by pathogenic bacteria to export virulence DNAs and/or proteins directly from the bacterial cytoplasm into the host cell. It forms a large multiprotein complex consisting of 12 proteins termed VirB1-11 and VirD4. VirB10 interacts with VirB7 and VirB9, forming the membrane-spanning 'core complex' (CC), around which all other components assemble. The CC is inserted in both the outer and inner membranes, playing a fundamental role as a scaffold for the rest of the T4SS components and actively participating in T4S substrate transfer through the bacterial envelope via conformational changes regulating channel opening and closing. In Gram-negative bacterial pathogen Helicobacter pylori, an important aetiological agent of gastroduodenal disease in humans, the comB3 gene encodes protein with best homologies to TraS/TraB from the Pseudomonas aeruginosa conjugative plasmid RP1 and TrbI of plasmid RP4 and VirB10 from the Ti plasmid of Agrobacterium tumefaciens, as well as DotG/IcmE of Legionella pneumophila.
Statistics
?
PSSM-Id: 319754
View PSSM: cd16426
Aligned: 519 rows
Threshold Bit Score: 36.7469
Threshold Setting Gi: 332995601
Created: 29-Mar-2013
Updated: 18-Aug-2016
Structure
?
Program:
Drawing:
Aligned Rows:
 
oligomer
Conserved site includes 66 residues -Click on image for an interactive view with Cn3D
Feature 1:oligomer interface [polypeptide binding site]
Evidence:
  • Comment:VirB8 interacts with VirB7 and VirB9, forming the membrane-spanning 'core complex' (CC), around which all other components assemble
  • Structure:3JQO: Escherichia coli outer membrane complex of a type IV secretion system VirB10; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          ##     ##   ## ####           # ##      ### #           # # # #     #    # # #
3JQO_A        43 TVPKg----KXIPCGTgTELDTt--------vPGQVSCr--VSQDVYSadglvrlidkGSWVDGQItGGIKdgqARVFVL 108
gi 332995601 219 TLPKd---aTLTDGLAlTALIGripvegqtpdPYPVKIy--IGRENLLanghelpeveGMIFSGLGfGDWNl--SCVSAR 291
gi 336481236 180 VIPAg----TIVRCVVvSGASVstgig-tpmgSRKILLk--PTSNGWLpagvr-valkDSLIMCSAtANLAe--ERVYVR 249
gi 431053709 207 QLPV-----GFMKAKLlVGVNAltgef-gsenPQTLMFr--VQAPAQLpnfik-mnlrGCFNISNVsGNLSs--ERIIVL 275
gi 500030876 248 WLSAg----TVIPTVLlTGQHVptsss-aqsnPAPVLLk--IVDVGVMpngft-idlvGCTVIGAArGSASt--RRVEIR 317
gi 502774921 177 YVPSn----SIFKAVLlTGVYAktstq-gksnPTPVIFr--LTDLSFFpnevr-rnlaGCYVGGEAhAELSd--ERVHTR 246
gi 548997893 273 RINAg----SLVPLTLlTGVDAptgk--raadKLTATFv--VTGEVRLpdgse-lnldPCRVLSDVqAHKTe--ARAYFR 341
gi 696171036 313 DIAL-----GLSRAIMlNGVDAsilgm-grqeDAPVMFs--IMSKMSIangey-tntkDCLVLGSAvGQMTv--QKAQIR 381
gi 751432442 320 KLPKmtlmaGFQRATLvTGADVptlq---kgnDLTRTVwfsTTGDMLIanght-enikECIIQAAAtGNFAs--ASADIR 393
gi 921096042 195 DLPV-----GFMKATLlVGINArsgef-gqsnPQQIVFn--VSAPAQLpnrik-lnlkGCFVVANAnGDLSa--KRIEAL 263
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          # # #      ######       #     ###         # #  #  # #  #                      
3JQO_A       109 WeRIRNDq----dGTIVNId-----sAGTNslGSAGIPGQVDAHXwERLRGAIXISLFSdtltalvnqtqs--------- 170
gi 332995601 292 LmSATFIfe--dgSIVNHSsagqplgYISDpsGVPCVAGSFVTNApTFLSQRVGLAAIGaagsayaeaqqqrqtsaltgt 369
gi 336481236 250 GdRMNLTyp--dgYTITTEiaa--yvSGED--GQEGVRCALIDKNwSSTFWAGLASGVGeigkamqsgnplldlpklgkt 323
gi 431053709 276 PvSIHCMtm--dgRYIVEGqvk-gfvSDRD--GKRDMSARVVSRAgRLLAGTVLAKSVEafanvignqsiqqnvsplgsi 350
gi 500030876 318 AeSISCIdq--rgRAIEQPfia--avQGQD--GHSGIPAKLVTLYgEVVKQSFWAGIYGtgaqtltqaatnrvsgdvvyd 391
gi 502774921 247 LtSFSCItsd-rrQVVDIPvtg--rvQGGD--GSVGIAGKVISKQeAALLLAGVSGFIEggaegfsaanstqnvnvfgts 321
gi 548997893 342 PyKLSCKfe---fGDLDMPlag--siSGPD--GALGIKGHLVNPAgKAVLYSTMTSGIDlaaawldseidtiela----- 409
gi 696171036 382 LdKISCIftnkegDKFIAEgqv--qgWASDenGQLGVQGVLITQEgKVLRASLPLAAVQtaldyitrsatnvvlpttgtp 459
gi 751432442 394 LtKISCSavdengKYYKLVgnvkgwvYGEN--GKQGLKGRLVTKEgELIEKAIPLALLEgaikalenstkssstvyaypg 471
gi 921096042 264 PvSMNCLtq--ngEYKITSsslkgfvQDAD--GKRGLDARVVSRArHLLASTLFAKTVTgfgkvisaqgftstntaigst 339
                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
Feature 1                                   ##########     # # ###  #   #    ##### 
3JQO_A       171 --------------nniqynstensgGQLASEALRSYxsipPTLYDQQGd-AVSIFv--aRDLDFS 219
gi 332995601 370 t----tstvsgdinklaageavqsatDEVSEWLLARQkqsfDAVVVNPGa-AVSVHl--dRSLPLD 428
gi 336481236 324 d---tsfiinldtlkqggiqggstalDKMTEHLLKEKeknaPFLILDSMr-HVDLIf--lHDCEIG 383
gi 431053709 351 -----ntikpdeavkngaatgvaggfTEVSKYLLNLAqqtsPALETGPGk-DVMLFi--qETAELT 408
gi 500030876 392 ------gtyfkntatsslaggsstafDKIADFYLQLAdearPYLEMHGGieGVDLIv--qRGVSLK 449
gi 502774921 322 e----qtytddqiarnaigkgvgksaGVLTDFFEDLLqeikPVIHAQGGq-EVELIi--sKGFSLE 380
gi 548997893 410 ----------ggdtlnsgggavasstAPLREYYLEEAkelqPYVQVRPLv-KASMVv--lDTVTLE 462
gi 696171036 460 t----gyqnlnasfgtggsnaasqtlGRLIQIYEKYLqnlnPVINVRGGr-EVTLMf--kGGEKIA 518
gi 751432442 472 ttgtstmnniqdsftegttktasttlDKFSDYYLMILeqlnPTIELKAGr-ETTIGfeggEVFQLE 536
gi 921096042 340 -----ttpndgevartafaggvaegfDDLGDYLLDLAkqiqPTLEHGAGk-NVLIWv--aEASTLK 397

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap