4EC6,4EC6,4EC6


Conserved Protein Domain Family
TraM-like

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cd16427: TraM-like 
Click on image for an interactive view with Cn3D
C-terminal domain of transfer protein TraM
This family contains the C-terminal domain of transfer protein TraM of the G+ broad host range Enterococcus conjugative plasmid pIP501 and similar proteins. The protein localizes to the cell envelope and is part of the plasmid transfer system that is accessible from outside of the cell. TraM displays a fold similar to the type IV secretion system (T4SS) VirB8 proteins from A. tumefaciens and B. suis (G-) and to the transfer protein TcpC from C. perfringens plasmid pCW3 (G+), reinforcing the prediction that TraM performs a key role in the secretion process, which is underlined by its surface accessibility. It is also structurally related to members of the nuclear transport factor-2 (NTF-2)-like superfamily with a high similarity to the NTF-2 protein from Rattus norvegicus. TraM (categorized as T4SS VirB8-like class GAMMA) does not possess the binding pocket of classic VirB8 class ALPHA proteins, recognized by VirB8 interaction inhibitors.
Links
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Statistics
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PSSM-Id: 319758
View PSSM: cd16427
Aligned: 53 rows
Threshold Bit Score: 76.8584
Threshold Setting Gi: 896026542
Created: 18-Aug-2016
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
trimer
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:trimer interface [polypeptide binding site]
Evidence:
  • Comment:structure, molecular dynamics, and cross-linking studies indicate that a TraM trimer acts as the biological unit; trimerization is facilitated by the N-terminal coiled-coil motif
  • Structure:4EC6: Enterococcus faecalis Ntf2-like (potential transfer protein Tram) homotrimer; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               ##  ## ##                     #                      ####             # #
4EC6_A        48 KETDISQSKIDTFGRYFLTYYFsqeknqeNYQSSLRtYVSEKVdisdWKALGKt--LKSVNYYGSEQTKKg---YSVEYL 122
gi 498440750 189 EKQVQQQAKIDTFSRFFLSNYYtgtkeddKVQEKIKrFVDKETl---KEFRGTeekIKSILPWEVKRDGSt---WQVSYV 262
gi 430645634 192 EELAARQPQIDTFSRYFITNYYsgemeeeRYRKKLQsFLAEKVakqiSPQKEK---VKSILSWEMKKKAKh---WQIAYV 265
gi 430479101 191 KEKVNSQGQLDTFCRYFLTNFYskesdkeVYQEKNKvFLSNRLidqlSPTETP---VKSILSWSIQPKKQq---WIVSYV 264
gi 739748692 274 TMIQDHEHEIDVMARYFLSYYYs------GDKDKLKaYLDNNDakftNPEVAT---VSSTMLEKVELVKNekdtYEVTYV 344
gi 514505176 112 HAQLEAQDKLHTFNRFFLTNYYsgtnaieKAKQKVKpFVSPEVek-kFSSSGSq--VKSISPWTLKKTKSg---WEATYV 185
gi 802124683 193 RKNDLYQSKIDTFGRYFLTNYFaqeknkeNYQSSIRrYVLNKVnisdWKMLGKt--LKNVNFYGSEKTKNg---YSVTYV 267
gi 486992599 182 QEQVHEQPQVDVVARFFLSSLYsgeentmSTQKKVRkYVTDEVi---EEIKGSkeqIRTLFLWASKKEGNq---WVLTYV 255
gi 642978700 211 LAIIEKQPTIDTTSRFFLASYFsev-gevQYKEGLKkFVGISMen-wDRPSGK---LNSLFLYKIRQEKKq---SKVSYL 282
gi 329570481 178 TEQVNEQNKVETFTRFFLTNYYsgktdakTRQAVLKkFVKKETlsdfLSEKSR---ARSMFPWEVKKKDKq---WTIAFI 251
                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
Feature 1         #         #              ###            
4EC6_A       123 LNVSvd-nrSKXQKITFEVEPTK---NGFLVTTQPKLTDFS 159
gi 498440750 263 INLQnnqekTTTQKVTFSIKEEE---KQYRVMTVPKEEPFE 300
gi 430645634 266 ITLEtekktQETKKVSFQLEEQG---KNYLVSTLPELDDFE 303
gi 430479101 265 ISLEesegkQVTKKVTFMLKEKN---KQYQVTQLPELENFE 302
gi 739748692 345 VGTTdeaqkTTVRRVTFNVKKKTsatQGWVVTTEPTTTAFA 385
gi 514505176 186 VSVQtkekvVETNRVQFTVQEKE---KNYQVTTLPKEEPFD 223
gi 802124683 268 LNVLvg-drSKMQKVTFEVEQTK---DSFLVKTKPILTDFS 304
gi 486992599 256 VTVKvakeeTETRQITVSMEKKE---NNYRVTKLPEEKEFT 293
gi 642978700 283 LTIQee-qtTQLKKIAFSVEIKE---GQVLVVSQPKIEEFS 319
gi 329570481 252 VVLInekdeQSTKKITFTAEETK---EQLVVMERPTEEDFE 289

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