3JQO,2BHV,3ZBI


Conserved Protein Domain Family
VirB10

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cd16429: VirB10 
Click on image for an interactive view with Cn3D
VirB10 forms part of core complex in Type IV secretion system (T4SS)
This family contains VirB10, a component of the type IV secretion system (T4SS), including homologs TrbI, TraF, TrwE and TraL. T4S system is employed by pathogenic bacteria to export virulence DNAs and/or proteins directly from the bacterial cytoplasm into the host cell. It forms a large multiprotein complex consisting of 12 proteins termed VirB1-11 and VirD4. VirB10, interacts with VirB7 and VirB9, forming the membrane-spanning 'core complex' (CC), around which all other components assemble. The CC is inserted in both, the outer and inner membranes, playing a fundamental role as a scaffold for the rest of the T4SS components and actively participating in T4S substrate transfer through the bacterial envelope via conformational changes regulating channel opening and closing. TrwE in R33 plasmid has been shown to be anchored to the inner membrane and its C-terminal is necessary for conjugation; the transmembrane domains of TrwB and TrwE are involved in TrwB-TrwE interactions. TraF protein of the RP4 plasmid is involved in circularization of pilin subunits of P-type pili. In gonococcal genetic island (GGI) of Neisseria gonorrhoeae, T4SS encodes TrbI and circularization occurs via a covalent intermediate between the C terminus of putative pilin protein TraA and TrbI.
Statistics
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PSSM-Id: 319755
View PSSM: cd16429
Aligned: 321 rows
Threshold Bit Score: 101.424
Threshold Setting Gi: 764630463
Created: 30-Mar-2016
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
oligomer
Conserved site includes 66 residues -Click on image for an interactive view with Cn3D
Feature 1:oligomer interface [polypeptide binding site]
Evidence:
  • Comment:VirB10, interacts with VirB7 and VirB9, forming the membrane-spanning 'core complex' (CC), around which all other components assemble
  • Structure:3JQO: Escherichia coli outer membrane complex of a type IV secretion system VirB10; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          ## ##   ## ####   # ##    ### #           # # # #       #    # # #  # # #  ###
3JQO_A        43 TVPKgKXIPCGTgTELDTTVPGQVSCrVSQDVYSadGLVRLIDkGSWVDGQIt--GGIKdgqARVFVLWeRIRNDqDGTI 120
gi 685121080 297 VAQG-TMISAVLeTAINTELPGNIRAhVIEPVFSfdGSRILLPaGTRLIGTFgnrDDIEq--ERVLIGWdRALTPdGKSI 373
gi 694205432 198 IIQG-TLIEATLeTAISSQLPGNVAAnVSYDVWSmdMSQVLIPrGSKLYGRYn--SQIErgqRRVMIAWdRLVTSdGQSV 274
gi 746507421 265 VTQG-TIIPANLdTAISSDLPGELKAtISRDVWStdATTVLIPrGSTLIGEYs--SDISlgqRRVLVAWnRVITTdKRTI 341
gi 780841630 298 LTQG-SVIQAALqTAINSDLPGSVVAvVSEPVPAfsGDRILIPrGSRLFGQYr--SGIDmhqKRILILWtRVLTPdGTSM 374
gi 798787339 223 VTQG-TMIQAVLeTAIDSTLQGPIRGlVTTDVVAydGSRILIPrGSRLIGSYs--ADVEtgqNRLLVAWeRIIMPdNQSV 299
gi 916934256 284 IVQG-TIISAVLeTAINTELPGNIRAqVTNPVYSfdGQSILMSaGTRLIGTYn--SKVSiaqKRVLIAWnRAITPeGKSV 360
gi 919159352 143 VPQG-TIIQASLeTAVDSTLPGPIRGvVSEDVHSmdGSAVLIPgGSKVFGEYs--SGIElgqERILIVWtRILTPsNRSV 219
gi 974140465 272 VTQG-SVIQASLeTAIDSSLPGPIRAvVTQDVHSydGMRILIPrGSRLIGRYs--ANIEtgqYRAMVAWeRIILPdNQSV 348
gi 974806705 309 VLQG-TLIQASLeTAIESTLPGQITAiVNYPVWSfdQSRVLIPeGSRLFGTYn--SNVSlgqARILVGWsRIVTPeGQSV 385
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ###  #     ###         # #  #  # #  #                                          #
3JQO_A       121 VNIDsAGTNslGSAGIPGQVDAHXwERLRGAIXISLFSdtltalvnqtqs----------------nniqynstensgGQ 184
gi 685121080 374 ALGS-IGTDllGRAGTEGNVDNHYaKKISAAVLVSTIGalpsiipamtggskssrdggatitveggglgntrrdgqvtSN 452
gi 694205432 275 QLEG-YGTDriGRAGMTGKVNNHTlARFGSAAAVSIIGalpdiavasieengg----------settrdtvesigqdaSD 343
gi 746507421 342 QIGS-RGVGplGRAGLAGNVQHHFaLKFEAAAFISIFTgisnlgsrtvnns---------------ddssfsdgatqfGE 405
gi 780841630 375 EIAA-VGGDhlGRSGLTGLVNTRFaERFGGAALISVIGaaptvaaestnne--------------ttsivlgdvgndlQD 439
gi 798787339 300 QISS-FGGDalGRSGTTGKVNNHFfRRLGAAALISTISaipsalqddsgdggvti-------stggsndpatgvsnavTG 371
gi 916934256 361 KLAA-TGVDrlGRGGQAGNVDSRFgQRFGTALLITSISaipsflanddatgnsa---------tqaatgvaqdssddlKE 430
gi 919159352 220 NIAS-YGADqlGRSGTGGAVDTRFgKRFGGAAAISIIGagpaiaankledd--------------dvstvvqgvgddfSS 284
gi 974140465 349 AIAA-YGGDaiGRAGLSGRVNKRFgERFGAAALISLLSsgpalasrdidne--------------tvrdgatrvssdlRD 413
gi 974806705 386 QLAA-FGADdqGRSGVTGSVNSRFgLRFGTAALLSIIGagpaiaaseasse--------------tgseiaedvagsfSQ 450
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|..
Feature 1        #########            # # ### #   #  ##### 
3JQO_A       185 LASEALRSYxs-------ipPTLYDQQGdAVSIFvaRDLDFS 219
gi 685121080 453 VASTVSGQSedilnkylsqpPVLRVAQGeEIRVFvnRDLIIR 494
gi 694205432 344 ALDNVLAEYld-------ipATISVDQGaVVMVRvnTDLEMF 378
gi 746507421 406 AASDPLTEYls-------ipPTIWIDHGeNINVF--VSRDLY 438
gi 780841630 440 AVGSVIADQvs-------iaPTIYVDQGaSVTVLvdRDVVIY 474
gi 798787339 372 ATESAIGKYvs-------mpPTITVNQGtRITVMvdRDLEIF 406
gi 916934256 431 TTEDVLEDYlk-------lpPIIRIPQGtQMNVLvnQDLDFT 465
gi 919159352 285 ATDTAIGSYin-------lpPTIHVRQGtSVTIIvdRDLEIF 319
gi 974140465 414 ATGNLVSDAls-------lpPVITINQGaRVTVMvdRDLEIF 448
gi 974806705 451 ATNAVIGEYat-------lpPVISVQPGaAISVIvdRDLEFY 485

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