Conserved Protein Domain Family
IcmE

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cd16431: IcmE 
DotG/IcmE is a homolog of VirB10 which forms part of core complex in Type IV secretion system
This family contains DotG/IcmE (VirB10 homolog) and a component of the type IV secretion system (T4SS), and similar proteins. The Dot/Icm system is a T4SS found in the pathogens Legionella and Coxiella and the conjugative apparatus of IncI plasmids; T4SS is employed by pathogenic bacteria to export virulence DNAs and/or proteins directly from the bacterial cytoplasm into the host cell. Similar to T4SS VirB/D components, the Legionella Dot/Icm secretion apparatus contains a critical five-protein sub-assembly that forms the membrane-spanning 'core-complex' (CC), around which all other components assemble. This transmembrane connection is mediated by protein dimer pairs consisting of two inner membrane proteins, DotF and DotG, each independently associating with DotH/DotC/DotD in the outer membrane.
Statistics
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PSSM-Id: 319757
View PSSM: cd16431
Aligned: 76 rows
Threshold Bit Score: 99.1894
Threshold Setting Gi: 748147358
Created: 30-Mar-2016
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
putative
Feature 1:putative oligomer interface [polypeptide binding site]
Evidence:
  • Comment:DotG/IcmE, along with DotF, independently associating with DotH/DotC/DotD in the outer membrane, to form the 'core complex' (CC)
  • Comment:based on comparison to similar proteins

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            ##  ##   ## ####    # ##     ### # # # # #         #    # # #  # # #  ######  
gi 975747294   208 LVSAy-TLVPAVMDTAMDSDdqNSIAIAh-VPTGAQAGAKFYSGQn----RLAG---DGIRIHFtGMELNg-VQCKVDay 277
gi 872380149   313 VTPG--TIYYAILEIGVNTDe-LSYVRSiiIQEGPLKGGVLLGMP-----ERRG---EKAVIMFnKLALNg-KDYSIN-- 378
gi 492084583   310 VVVG--QTFYSVLQIGVNTDe-ISPIRAvmVQKGPLEGAVLTGEP-----ARTG---EKAKLSFtDMSLNg-KSYKVT-- 375
gi 1004360081  236 LARAa-SQYMATTDMALNSDv-GGKFSVtiRGGGKLNGTKLMGSF-----ERKE---EYLRMEFdKMVTRt-DTYQVD-- 302
gi 476722134   246 AARAa-SMYMATTDLALNSDv-GGPVSIt-IQNGRLRGSKLLGTF-----ERKE---QWVRIDLnKMATKt-HTLPIK-- 311
gi 663682812   243 LARAg-DMYMATADLALNSDvgGSASLT--IRNGSARNFRLIGSF-----ERKE---EWIRLELnKLVTPwkTYTNVS-- 309
gi 936664133   928 RIPAhtLFSKAQIACEIDTDy-AGDTCVe-ITEGPLAGAVLRGTYevpnlDNPVmprDKVLVSLnKMVFNr-QSIDIE-- 1002
gi 574079503   107 IERGg-AMSIATADFAVNSDv-GGPISLt-LRSGKARGRQLIGQF-----ERKD---KWLRIELnKMLSEdmDPVPVK-- 173
gi 936657690   312 VFDAg-ELVYARTTLPIDSDi-PGPIRIr-LLTGKAEGSIAFGEM-----ELIEq-aPGVALTVtSVIRDg-KQIPVK-- 379
gi 120326735   309 LVKGg-EIRYAQTDIALNTDf-QGPVRMt-FLEGNLRGYIGMGTF-----ELNEl-gAKMKLTIdTLFDPdgQSYAVN-- 377
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1            #     ###            # #  #  # #  #                                          #
gi 975747294   278 gvASDS--LRA---AVSSNVNNRWfSRIILPAVANGLGRTGQL-YADSNSQmiitdggnay-rstdtpdgkAVAGTIIGG 350
gi 872380149   379 --AVALd-LETmrsGIADNVDRHTfERYMKLMGAAFISGYADS-LAGSTTKtysdgttet--iterlpdadDQIAYAIGR 452
gi 492084583   376 --AIALd-PDTyrsGLADGVDNHTfERYSKLMIASFVDGYSDA-LQDSQTTtntdgssst--vnsalpnagDQVKMGIGK 449
gi 1004360081  303 --AIGLdiDSTl-nAVEGQVNRHTlYRYGWWGVGSVLGAIGKA-AEANANTstylagnngtviqdtkrdskREVKIAVGE 378
gi 476722134   312 --AIGLdlDTTl-nAVQGEVDEHLlYRYGWWGVGTVLSAIGSA-AEKNADSniiitdgsv--iksmqadssRELKSALGE 385
gi 663682812   310 --AIGLd-VGTslnAVEGDVDRHImYRYGWWGFGTVLKAVGSA-AEKNANQqvvisdgna--iqstqqdssREIKMALGS 383
gi 936664133  1003 --AVVVn-PITmsqAVSSEVDYHRmYRWGGLMAATTLDLAQGLvIGNAIAEgasseds-------gsatnaVAVQMAIAE 1072
gi 574079503   174 --AIGLd-METtmsGVEGDLDSHImYRYGWWGLGAVLSAVGKA-AENNANEqvivsdgav--vtsaqqdssRELKMALGS 247
gi 936657690   380 --AWVLs-AETekaLIEGEVDHHYiQRFGGMFAGLFMTGFLDS-LIDTTVTtsngettts---tgaidntrDRIVYSIAK 452
gi 120326735   378 --GYVLd-PNTtlwAMASNVDYHIiYRYGGFGLGTILSAFQTL-AENRAQIsetvtpdgtrstqnrepdgkQVTWTVLGE 453
                          170       180       190       200
                   ....*....|....*....|....*....|....*....|....*..
Feature 1          #########          # # ### #   #         ##### 
gi 975747294   351 MGEQAGRVLADdaarlPIKQVTVDRNqLIGIQf-------vAPVYES 390
gi 872380149   453 AGEKLVPIFERef--dRDPTVEVDSNrEIAIMl-------lNGIQIE 490
gi 492084583   450 MGEKFSPIFEKef--dRPPTVTVEPNkSIVIMfm-----anLDLSKP 489
gi 1004360081  379 LGQDMGDVFKSri--nRPITVKLNAGdSIGIFl--------LDDICQ 415
gi 476722134   386 LGSEIGSVMKEri--nRPITVSLKVNdELGVFf-------lDDVCLP 423
gi 663682812   384 LGNDLGSAFQDrl--dRPITVSLKVGdEVGVFf--------LDDVCR 420
gi 936664133  1073 PIKETSDMYRAqm--gRKPTARMFKDdPVGVLf-------tSEVIDS 1110
gi 574079503   248 LGQDLGDAFKDri--nRPITVSLKVNeQVGVYf-------lDDVCAS 285
gi 936657690   453 AAQGFLPILYDna--rRPVQVEIPKGqTMYLLfenq-vletDEIQTP 496
gi 120326735   454 FGALFEETFRDni--nRPITVTLDPNeEAGVLfedtvcelnTDISKK 498

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