1CHD,1A2O,3SFT


Conserved Protein Domain Family
CheB_Rec

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cd16432: CheB_Rec 
Click on image for an interactive view with Cn3D
Chemotaxis response regulator protein-glutamate methylesterase, CheB, with N-terminal REC domain
This family contains the methylesterase CheB (EC 3.1.1.61; also known as CheB methylesterase, chemotaxis-specific methylesterase, methyl-accepting chemotaxis protein methyl-esterase, or protein methyl-esterase) domain with a REC domain at the N-terminus. CheB is a phosphorylation-activated response regulator involved in reversible modification of bacterial chemotaxis receptors. Signaling output of the chemotaxis receptors is modulated by CheB and methyltransferase CheR by controlling the level of receptor methylation. The N-terminal regulatory (REC) domain blocks the active site of the C-terminal domain until it is phosphorylated. Reversible methylation of transmembrane chemoreceptors plays an important role in ligand-dependent signaling and cellular adaptation in bacterial chemotaxis. Phosphorylated CheB catalyzes deamidation of specific glutamine residues in the cytoplasmic region of the chemoreceptors and demethylation of specific methyl glutamate residues introduced into the chemoreceptors by CheR.
Statistics
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PSSM-Id: 319751
View PSSM: cd16432
Aligned: 706 rows
Threshold Bit Score: 132.103
Threshold Setting Gi: 469478068
Created: 4-Jan-2016
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
heterodimer
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:heterodimer interface [polypeptide binding site]
Evidence:
  • Comment:The N-terminal regulatory (REC) domain blocks the active site of C-terminal CheB domain until it is phosphorylated
  • Structure:1A2O: Salmonella enterica CheB and REC domain interactions; contacts at 4.0A
    View structure with Cn3D
  • Citation:PMID 9465023

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1               #  ##  #                    #  #  ##  #                                  
1CHD_A        12 LIAIGASTgGTEAIRHVLQPLPLSs-pAVIITQHMPpgFTRSFAERLNKLcQISVKEAEDg-ERVLp-GHAYIAPGdkHM 88
1A2O_A       158 LIAIGASTgGTEAIRHVLQPLPLSs-pAVIITQHMPpgFTRSFAERLNKLcQISVKEAEDg-ERVLp-GHAYIAPGdkHM 234
gi 159882011 162 IVAIGISTgGPATLIDILQDCEVDeetAYLVVQHMPpqFTPSLAKRLSEYtPIDFFHASHg-QAVLg-GHGYLAPGgwHM 239
gi 394454480 172 IIVIGASTgGTQAVEQIIRQLDPRlkaAVLVAIHLPpkFTHSYSRRLKGMtELTVLEGRT--GLIPkpGKVIIAPGgrNM 249
gi 586956479 155 VVVIGGSTgGAPAVEAVIRNLPSGfpwAVLVAVHLPahFTDSLVDRLRRAtPLPLAAAGSs-SRLEa-GQILVAPGghNL 232
gi 670938244 136 LVIVGGSTgGTLAVEQLVRALPTTlacAVLVAVHLPasFLSSFVDRLRRAtALPVVPGGAg-VVLQp-GQIVVAPGgrNS 213
gi 746974585 157 IIAIGASTgGPSAVEYIVCNIPRNlsiPVIIAQHMPdrFIETYAVRLANEtGLPVSVAREgaSLLP--GHIYLAPGtsNI 234
gi 808185324 168 VIVIGASTgGTQAVESIVKQLSPDlnaSVLVALHLPqnFTRTYSERLRSLtPLKVVEGHRg-VKLCp-GKIIVAPGgcNM 245
gi 816390875 183 LIVIGTSTgGPRTLKAILKHIPKDfpsPILMVQHLDafFMKQFSNSLDQIcQLDVKIPEDg-EKIYp-GTAYLSPGgkHM 260
gi 887499968 177 VIVLGASTgGSTAIEYLIKDLHLKqpaVVLVAVHMPekFTKRLAQRLQKLtKWRVEEGTQg-MVLAp-QTIIIAPGgnNM 254
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                                              #                       ##
1CHD_A        89 ELARsg----anYQIKIHDGppv--nrhRPSVDVLFHSVAKHagRNAVGVILTGMgNDGAAGMLAMYQa-GAWTIAQNEA 161
1A2O_A       235 ELARsg----anYQIKIHDGppv--nrhRPSVDVLFHSVAKHagRNAVGVILTGMgNDGAAGMLAMYQa-GAWTIAQNEA 307
gi 159882011 240 KVTPd-------KKIKLFKDdsy---iyYPSVDVLFDSVAEVycPNAVGVLLTGIgRDGAEGLLKMKQr-GCITIAESEE 308
gi 394454480 250 IVHTvmgn-assLKIGFSEDsga--ehdLPSVDKLMQAVAQSgvRRVIGVILTGMgKDGTAGAKAIALrkGGHVIAQDEA 326
gi 586956479 233 VIRPvt----dsPWLGWQTDfvteasldVPSVDILMQSVARLvgRNVLGVVLTGLgHDGTAGARSIRQh-GGTVIVQDEA 307
gi 670938244 214 LVSAatnspwqaWQTEFTAEpsp--sgdEPSLDVLMRSAARTvgRNVLGVVLTGLgRDGTLGAQAIRQh-GGTVLVQDEA 290
gi 746974585 235 RINNdd----rsPVVQFVTDhyk--efnSPSVDCLFESVADVygKRGIGVILTGMgKDGTAGLIKMKAa-GALTIAQDEA 307
gi 808185324 246 VVESimgs-renLMVSFTDEepv--lydQPSIDLLMRSVAQSavKQVVGVILTGMgKDGTSGASYIRSr-GGIVVAQNEE 321
gi 816390875 261 KVVLrn----niPHIKTFVGkpv--nfcMPSVDVLFFSAAEIykDDTLGVLLTGLgDDGAAGLNAIKNa-GGKTIAESEE 333
gi 887499968 255 RVTHhpfw-pnlLSLELAPSqa----ldSPSVNELMTSAAHCarEQVLGVIMTGMgQDGSIGAKEIIRk-GGIVIAQDEA 328
                        170       180       190
                 ....*....|....*....|....*....|....*
Feature 1          #                                
1CHD_A       162 scVVFGMPREAINMGg--vSEVVDlsQVSQQMLAK 194
1A2O_A       308 scVVFGMPREAINMGg--vSEVVDlsQVSQQMLAK 340
gi 159882011 309 taVVYGMPAAAKEIGa--aMEILPayRIASAIKRH 341
gi 394454480 327 ssAIFGMAKSAIESGn--tDKVLPlsKIAQYLNRY 359
gi 586956479 308 ssAVFGMPKSVIQAGl--aSEVLPlsSMTDFMVRH 340
gi 670938244 291 ssAVFSMPKSVIQAGw--aNAVLPlhELPKAIGRH 323
gi 746974585 308 ssVVYGMPKAAFDSGa--aVYQLPlhEIPNFIISG 340
gi 808185324 322 tsAIFGMAKSAIDSGy--iNKVLPlpEIPQFLNRF 354
gi 816390875 334 tcVVYGMPKSAVKLGa--aQFIVPsyRVKDYLINF 366
gi 887499968 329 tsSIYGMAKAAVENGvvngEFALG--QINSIINRF 361

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