3A7R,3RIR,1W66,2P0L,2QHT


Conserved Protein Domain Family
BPL_LplA_LipB

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cd16435: BPL_LplA_LipB 
Click on image for an interactive view with Cn3D
biotin-lipoate ligase family
This family includes biotin protein ligase (BPL), lipoate-protein ligase A (LplA) and octanoyl-[acyl carrier protein]-protein acyltransferase (LipB). Biotin is covalently attached at the active site of certain enzymes that transfer carbon dioxide from bicarbonate to organic acids to form cellular metabolites. Biotin protein ligase (BPL) is the enzyme responsible for attaching biotin to a specific lysine at the active site of biotin enzymes. Biotin attachment is a two step reaction that results in the formation of an amide linkage between the carboxyl group of biotin and the epsilon-amino group of the modified lysine. Lipoate-protein ligase A (LplA) catalyses the formation of an amide linkage between lipoic acid and a specific lysine residue in lipoate dependent enzymes.
Statistics
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PSSM-Id: 319740
View PSSM: cd16435
Aligned: 5 rows
Threshold Bit Score: 172.722
Threshold Setting Gi: 384482262
Created: 28-Mar-2013
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitecatalytic site
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3A7R: Escherichia coli lipoate-protein ligase A with bound lipoyl-AMP; contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                      10        20        30        40        50        60        70        80
              ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                    ##                     #     ####                              ##
3A7R_A      4 RLLISDSydp---wfNLAVEECIFrqmp--atqRVLFLWRNadTVVIGraqnpwkecntrrm--eednvrlarrssggGA 76
3RIR_A     84 FSEVYDSid-----sTQLAAKKSLvgn----qsSFFILSDEqtKGRGRf----------------------------nRH 126
1W66_A     15 DVRQLGTvdy---rtAWQLQRELAdarv-aggaDTLLLLEHpaVYTAGrrtetherpid--------gtpvvdtdrggKI 82
2P0L_A     14 SIFKDYVtdaqdaekPFIWTEVFLreinrsnqeIILHIWPXtkTVILGxldrelphlelakkeiisrgyepvvrnfggLA 93
2QHT_A      4 LVEDLGLvpy---geAWAYQKRVHrevvagnrpPTLLLLEHprVITLGrkatgenllfpeswy-rengfelywverggDV 79
                      90       100       110       120       130       140       150       160
              ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1     ###      ###                                                 ##          #  ### 
3A7R_A     77 VFHDl-gNTCFTFMAgkp-------eydKTISTSIVLNALNALgv-sAEASG--------RNDLVVktvegDRKVSGSAY 139
3RIR_A    127 WSSSkgqGLWMSVVLrpnvaf-smiskfNLFIALGIRDAIQHFsqdeVKVKW--------PNDIYId----NGKVCGFLT 193
1W66_A     83 TWHGp-gQLVGYPIIglaepl--dvvnyVRRLEESLIQVCADLgl-hAGRVDg-------RSGVWLpg-rpARKVAAIGV 150
2P0L_A     94 VVADe-gILNFSLVIpdvferklsisdgYLIXVDFIRSIFSDFyq-pIEHFEvetsycpgKFDLSIn----GKKFAGLAQ 167
2QHT_A     80 TYHGp-gQLVGYPIFpvgre----vrrfLRQIEEAIVRVAAGYgi-sAYPTPg-------YAGVWVg----EDKLCAIGV 142
                     170       180       190       200       210       220       230       240
              ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                   ### #                                                             
3A7R_A    140 REtk-----drGFHHGTLLLnadlsrlanylnpdkkklaa-------kgitsvrsrvtnltellpgiTHEQVCEAITEAF 207
3RIR_A    194 EMvanndgieaIICGIGINLtqqlenfdesirh---------------------ratsiqlhdknklDRYQFLERLLQEI 252
1W66_A    151 RVsr-----atTLHGFALNCdcdlaaftaivpcgis----------------daavtslsaelgrtvTVDEVRATVAAAV 209
2P0L_A    168 RRik-----ngIAVSIYLSVcgdqkgrsqxisdfykiglgdtgspiaypnvdpeixanlsdlldcpxTVEDVIDRXLISL 242
2QHT_A    143 AVke-----gvSFHGFALNVntdlndftvivpcglk----------------gkgvtslekllgrkvPMEEAKARVVAAF 201

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