2P0L,2E5A,2P5I,3R07,3A7R,1VQZ


Conserved Protein Domain Family
LplA

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cd16443: LplA 
Click on image for an interactive view with Cn3D
lipoate-protein ligase
Lipoate-protein ligase A (LplA) catalyzes the formation of an amide linkage between free lipoic acid and a specific lysine residue of the lipoyl domain in lipoate dependent enzymes, similar to the biotinylation reaction mediated by biotinyl protein ligase (BPL). The two step reaction includes activation of exogenously supplied lipoic acid at the expense of ATP to lipoyl-AMP and then transfer to the epsilon-amino group of a specific lysine residue of the lipoyl domain of the target protein.
Statistics
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PSSM-Id: 319742
View PSSM: cd16443
Aligned: 62 rows
Threshold Bit Score: 130.068
Threshold Setting Gi: 378979677
Created: 4-Apr-2016
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitecatalytic site
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1                                              #                                   #    #
2P0L_A        14 SIFKDYVtdaqdaekPFIWTEVFLreinrsnqeIILHIWPXt-kTVILGXLDrelphlelakkeiisrgyepvvRNFGGL 92
2E5A_A         6 LILQSISndv---yhNLAVEDWIHdhmn-legkPVLFLWRNs-pTVVIGRHQnpwqecnlnl--mreegvklarRRSGGG 78
gi 81789994    6 CIFLDLRghs--ilhQLQIEEALLrvan-qnfcIINSGAKD---SIVLGISRnlnqdvhisr--aqadhipiirRYSGGG 77
gi 166154714   5 CVFVHCEglp--ifkQLQLEEALLrts---sqnFCLVNTHLp-eAVVLGISRkperdlhveh--lkedgipiirRYSGGG 76
gi 338810393  10 RWMDSGTyqds-plePLIRDEALAasmqsesaaPVIHLWVYd-qALYLGRRDaklphlqqalhqfgqdgfgcvlRSSGGA 87
gi 32474618   16 ILARRQLarde-amlQWADEVVGDnest-tidtESLRMWQFdqpTVVLGRSSkindevnrpw--cqehrievlrRCTGGA 91
gi 380031498  48 ATLSQKYepaa-mldSFAYTNALLwlta-arqqPIVHFWQLq-pTVILGLLDqrlprltagladlhaagyqimlRNSGGL 124
gi 378979677  21 LQFCEDPtqa--eqpLFAKASAGE---------AVAQLWQAp-qGLVVPGSYrqftdlpaisahfaargwpvwlRRSGGG 88
gi 124078501   3 FEFIDAAhagqagsdPLQDELALLdlaa--qgrQVAQLWEAp-lSLVVPRTYlrhaaletaradfaqqgcpvflRMSGGG 79
gi 300784010   4 GSDVRAAftd--paeNLAFDEALLrva---pesPVLWLWRNp-vCVVVGRGQriarevride--cvrdgvpvlrRASGGG 75
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        ####   # #                                                     #            ####
2P0L_A        93 AVVADegILNFSLVIpdvferk-lsisdgYLIXVDFIRSIFSDFyq---pIEHFEvetsycpgKFDLSIn----GKKFAG 164
2E5A_A        79 TVYHDmgNINLTFFTtkk--------kydRMENLKLVVRALKAVhph-ldVQATK--------RFDLLLd---gQFKISG 138
gi 81789994   78 TVFIDsnTLMVSWIMnsse------asaqPQELLAWTYGIYSPLlp---nTFSIR--------ENDYVLg----HKKIGG 136
gi 166154714  77 TVFLDadSLMVSWIInspt------pspsSKDLLQWTQDIYAPIfp---tGFKIT--------ENDYTFl----DKKIGG 135
gi 338810393  88 CVPLDagVLNMACLLpnt--------sisIDSFFSFVAQLLDVGlrdygkLEFGEvtgsycagEYDFSIh----GKKIGG 155
gi 32474618   92 SVVGGpgCWMYSVVLrlldetsirkidvaHDYVMQHVLAAVQTQlp---nAERKG--------ICDLTFe----NRKFSG 156
gi 380031498 125 AVVADpgVLNVSLFLpatr------erypITAAYQLMVDYVQAVwpq-lnITTGEitrsycpgDYDLSIa----GRKIAG 193
gi 378979677  89 LVPQGpgIINLSLAWpvqqplg-eaaepiYHSLCAVLQRTLARFgv---aSHPRAvsgsfcdgRYNLACgegaeARKIVG 164
gi 124078501  80 LVPQGpgILNLSLAYtveqppg-arsdavYLHLCEVIGDALQSLgv---dTHWQAvagsfcdgRYNLAWgppeaARKIAG 155
gi 300784010  76 TVFHDpgNLNVTLVLpgpa-------drpLEALGQVMSAAVDQLg----lVPRIG--------DRGLFVg----DAKLCG 132
                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1        #                       ### #                                                   
2P0L_A       165 LAQRRik------------ngIAVSIYLSVcgdqkGRSQXISDFYkiglgdtgsp-iaypnvdpeIXANLSDLldc---- 227
2E5A_A       139 TASKIgr------------naAYHHCTLLCgtdgtFLSSLLKSPYqgirsna-------tastpaLVKNLMEKdp----- 194
gi 81789994  137 NAQYIqr------------hrWVHHTTFLWdidldKLSYYLPIPQqqpty----------rnqrsHEEFLTTLrpw---- 190
gi 166154714 136 NAQYIqk------------yrWVHHTTFLWnmnpkKLARYLPTPEiqpsy----------rqnrsHDEFLTTIyel---- 189
gi 338810393 156 MAQRRtr------------hgSILQLCINVdd--rPRGEWMEHFYrlagldemqshkpipsidasTVGSIASLtgk---- 217
gi 32474618  157 NSLRVar------------hhLLYHGTVLVdsdldLIQSCLEFAPrqpdyre-------qrshreFIGNIDVDvs----- 212
gi 380031498 194 MSQRRta------------eaLVIMLYVSVdgdqmARSQLIQRFYqvslaghhd---arfpdvdpQVMTTVADala---- 254
gi 378979677 165 TAQYWrplaa------ggghvVLAHAVILIdadlsAAHQAANAFEaqlgse--------rvycadKTVTLAQLlpgerhl 230
gi 124078501 156 TAQYWrrapaamqtadgqrhlVLAHAVLLVsagpvQINARANAFEaaidsg--------rhydagKVVSVREAllasg-r 226
gi 300784010 133 FAVFRtk------------tgLLAHSTLLVetsagLVGRYLTGAPpdprpl---------dshrsPVASLAEHglrp--- 188
                        250
                 ....*....|....*.
Feature 1                        
2P0L_A       228 -pxTVEDVIDRXLISL 242
2E5A_A       195 -tlTCEVVINAVATEY 209
gi 81789994  191 -fpSRDDFLERIKASG 205
gi 166154714 190 -fdSREDFLSQLKQSA 204
gi 338810393 218 -laTMDDVKARLLETI 232
gi 32474618  213 --qLTSDLAKQFGALS 226
gi 380031498 255 -apTDTTATQARFIAI 269
gi 378979677 231 lprFSETLAQELDAAR 246
gi 124078501 227 avdDDAALMAQVSDAL 242
gi 300784010 189 gfpAVEAAVRAAASQL 204

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