Conserved Protein Domain Family
mRING-C3HGC3_RFWD3

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cd16450: mRING-C3HGC3_RFWD3 
Modified RING finger, C3HGC3-type, found in RING finger and WD repeat domain-containing protein 3 (RFWD3) and similar proteins
RFWD3, also known as RING finger protein 201 (RNF201) or FLJ10520, is an E3 ubiquitin-protein ligase that forms a complex with Mdm2 and p53 to synergistically ubiquitinate p53 and acts as a positive regulator of p53 stability in response to DNA damage. It is phosphorylated by checkpoint kinase ATM/ATR and the phosphorylation mutant fails to stimulate p53 ubiquitination. RFWD3 also functions as a novel replication protein A (RPA)-associated protein involved in DNA replication checkpoint control. RFWD3 contains an N-terminal SQ-rich region followed by a RING finger domain that exhibits robust E3 ubiquitin ligase activity toward p53, a coiled-coil domain and three WD40 repeats in the C-terminus, the latter two of which may be responsible for protein-protein interaction. The RING finger in this family is a modified C3HGC3-type RING finger, but not a canonical C3H2C3-type RING-H2 finger or C3HC4-type RING-HC finger.
Statistics
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PSSM-Id: 319364
View PSSM: cd16450
Aligned: 30 rows
Threshold Bit Score: 76.9313
Threshold Setting Gi: 551652855
Created: 6-May-2013
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
modified RING
Feature 1: modified RING finger (C3HGC3-type), 8 residue positions
Conserved feature residue pattern:C C C H G C C CClick to see conserved feature residue pattern help
Evidence:
  • Comment:consensus of the canonical RING-HC/RING-H2 fingers: C-X2-C-X(9-39)-C-X(1-3)-C/H-X(2-3)-H-X2-C-X(4-48)-C-X2-C, X is any amino acid and the number of X residues varies in different fingers.
  • Comment:A RING finger typically binds two zinc atoms, with its Cys and/or His side chains in a unique "cross-brace" arrangement.
  • Comment:The RING fingers found in RFWD3 and its homologs are modified as C3HGC3-type RING finger, rather than canonical C3H2C3-type RING-H2 finger, or C3HC4-type RING-HC finger.
  • Comment:putative Zn2+ binding site

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
Feature 1            #  #                # #  #  #               #  # 
gi 126253679 283 EGDTCTICLEQWTNAGdHRLSALRCGHLFGYRCISTWLKgq----vrKCPQCN 331
gi 148907978  87 ATSSCSICMEPWTSGGnHRISCLPCGHLFGRPCIIKWIRqrgg-nigKCPQCN 138
gi 303280643  93 GALSCSICLEPCALDGaHQVSALSCGHCFGHACISKWLTrhkkgnggKCPQCN 145
gi 313234972  26 DSDICSICFEVWTSTGsHRVCSLKCGHLFGHSCVKTWLIss-----pRCPECN 73
gi 449678343  92 EEQSCPVCFEEWTSSGkHRLCSLNCGHLFGRSCIERWIKeqr---nkNCPQCK 141
gi 546306122 117 DDNLCIICLENWTSAGpHQICCLPCGHLFGYACIRQWLGrsr--rgrQCPNCK 167
gi 290982653 366 PNNICSICLSAWSSTGlHQVCCLSCGHLFGKNCIEKWLKkn-----sTCPKCK 413
gi 42567616  116 EGLSCSICMEVWTSGGqHQVCCLPCGHLYGYSCINKWFQqrr--sggKCPLCN 166
gi 19074263    3 CGTICPICFSEYTTAGnHRIVSLQCGHLFGSQCIEKWIGkkt---kmQCPLCS 52
gi 551652855   7 GNDTCMICFEQWTNTGeHRVVCLGCGHLFGASCIQKWLSer-----kHCPTCN 54

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