2ECL


Conserved Protein Domain Family
RING-H2_RBX2

?
cd16466: RING-H2_RBX2 
Click on image for an interactive view with Cn3D
RING finger, H2 subclass, found in RING-box protein 2 (RBX2) and similar proteins
RBX2, also known as CKII beta-binding protein 1 (CKBBP1), RING finger protein 7 (RNF7), regulator of cullins 2 (ROC2), or sensitive to apoptosis gene protein (SAG), is an E3 ubiquitin-protein ligase that protects cells from apoptosis, confers radioresistance, and plays an essential and non-redundant role in embryogenesis and vasculogenesis. It promotes ubiquitination and degradation of a number of protein substrates, including c-JUN, DEPTOR, HIF-1alpha, IkappaBalpha, NF1, NOXA, p27, and procaspase-3, thus regulating various signaling pathways and biological processes. RBX2 is necessary for ubiquitin ligation activity of the multimeric cullin (Cul)-RING E3 ligases (CRLs). RBX2-containing CRLs are involved in NEDD8 pathway and RBX2 specifically regulate NEDD8ylation of Cul5. It can bind and activate HIV-1 Vif-Cullin5 E3 ligase complex in vitro. It is also a substrate of NEDD4-1 E3 ubiquitin ligase and mediates NEDD4-1 induced chemosensitization. The inactivation of RBX2 E3 ubiquitin ligase activity triggers senescence and inhibits Kras-induced immortalization. Endothelial deletion of RBX2 causes embryonic lethality and blocks tumor angiogenesis, suggesting a way for anti-angiogenesis therapy of human cancer. Moreover, as a component of Cullin 5-RING E3 ubiquitin ligase (CRL5) complex, RBX2 regulates neuronal migration through different CRL5 adaptors, such as SOCS7. Furthermore, RBX2 functions as a redox inducible antioxidant protein that scavenges oxygen radicals by forming inter- and intra-molecular disulfide bonds when acting alone. RBX2 contains a C-terminal C3H2C3-type RING-H2 finger that is essential for its ligase activity.
Statistics
?
PSSM-Id: 319380
View PSSM: cd16466
Aligned: 21 rows
Threshold Bit Score: 91.425
Threshold Setting Gi: 196011403
Created: 2-May-2013
Updated: 18-Aug-2016
Structure
?
Program:
Drawing:
Aligned Rows:
 
Zn binding siteRING-H2 finger
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Structure:2ECL; Homo sapiens Ring-box protein 2 binds three Zn2+ ions through its RING-H2 finger.
    View structure with Cn3D
  • Comment:Homo sapiens Ring-box protein 2 contains a third Zn2+-binding site, in addition to the two Zn2+ ions of the canonical RING-H2 finger.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
Feature 1           #  #       #  #             #      # #  # ##          #  #    
2ECL_A        15 CDTCAICRvqVMDACLRCQAENKq-----eDCVVVWGECNHSFHNCCMSLWVKqNNRCPLCQQDW 74
gi 24652785   42 CDICAICRvqVMDSCLRCQADNKrdvmgrqDCVVVWGECNHSFHHCCMSLWVKqNNRCPLCQQEW 106
gi 313235958 168 CDICAICRvvVTEPCLKCQSSGKga----aDCAVVWGECNHSYHNCCMSRWVAtTPRCPLCQQDW 228
gi 74961633   47 CDTCAICRvhLMEECLRCQSEPSa------ECYVVWGDCNHSFHHCCMTQWIRqNNRCPLCQKDW 105
gi 390344073  66 CDTCAICRvqVMDACLRCQTENKq-----eECVVVWGDCNHSFHNCCMSLWVKqNNRCPLCQQEW 125
gi 260807866  36 CDTCAICRvqVMDACLRCQAENKq-----eDCVVVWGECNHSFHNCCMSLWVKqNNRCPLCQQEW 95
gi 295293375  44 CDTCAICRvqVMDACLRCQGENKq-----dDCVVIWGECNHSFHNCCMSLWVKqNNRCPLCQQEW 103
gi 156398281  33 CDTCAICRvqVMDACLRCQSDNKq-----eECVVVWGECNHSFHNCCMSLWVVqNNRCPLCQQEW 92
gi 443709057  19 CDTCAICRvqVMDACLRCQSENKq-----eECVVVWGDCNHSFHNCCMSLWVKqNNRCPLCQQEW 78
gi 761901073  66 CDTCAICRvqVMDACLRCQSDKKq-----eECIVVWGECNHSFHNCCMQLWIKqNNRCPLCQQEW 125

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap