3DPL,2LGV,1LDJ,1U6G,4A0C,4P5O,3DQV


Conserved Protein Domain Family
mRING-H2-C3H2C2D_RBX1

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cd16485: mRING-H2-C3H2C2D_RBX1 
Click on image for an interactive view with Cn3D
modified RING finger, H2 subclass (C3H2C2D-type), found in RING-box protein 1 (RBX1) and similar proteins
RBX1, also known as Hrt1, protein ZYP, RING finger protein 75 (RNF75), or regulator of cullins 1 (ROC1), is an E3 ubiquitin-protein ligase necessary for ubiquitin ligation activity of the multimeric cullin (Cul)-RING E3 ligases (CRLs). RBX1-containing CRLs are involved in NEDD8 pathway and RBX1 specifically regulate NEDD8ylation of Cul1-4. It can also bind and activate HIV-1 Vif-Cullin5 E3 ligase complex in vitro. Moreover, RBX1 is an essential element of Skp1/Cullins/F-box (SCF) E3-ubiquitin ligase complex that targets diverse proteins for proteasome-mediated degradation. It is a direct functional target of miR-194 and plays an important role in proliferation and migration of gastric cancer (GC) cells. RBX1 is also an essential component of KEAP1/CUL3/RBX1 E3-ubiquitin ligase complex that functions as a regulator of NFE2-related factor 2 (NRF2) and plays a key role in NRF2 pathway deregulation in multiple tumor types, including ovarian carcinomas (OVCA) and papillary thyroid carcinoma (PTC). Furthermore, RBX1 associates with DDB1, Cul4A, and Fbxw5 to form the Fbxw5-DDB1-Cul4A-Rbx1 complex that may function as a dual SUMO/ubiquitin ligase suppressing c-Myb activity through sumoylation or ubiquitination. RBX1 contains a C-terminal modified RING-H2 finger that is C3H2C2D-type, rather than the canonical C3H2C3-type. The modified RING-H2 finger is essential for its ligase activity.
Statistics
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PSSM-Id: 319399
View PSSM: cd16485
Aligned: 57 rows
Threshold Bit Score: 103.988
Threshold Setting Gi: 74664250
Created: 2-May-2013
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Zn binding sitepolypeptidemodified
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Structure:2LGV; Homo sapiens RBX1 binds three Zn2+ ions through its modified RING-H2 finger.
    View structure with Cn3D
  • Comment:Homo sapiens RBX1 contains a third Zn2+-binding site, in addition to the two Zn2+ ions of the canonical RING-H2 finger.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
Feature 1          #  #       #  #            #      # #  # ##          #  #    
3DPL_R        38 DNCAICRNHIMDLCIECQAnqas-atseECTVAWGVCNHAFHFHCISRWLKTRqvCPLDNREW 99
2LGV_A        32 DNCAICRNHIMDLCIECQAnqas-atseECTVAWGVCNHAFHFHCISRWLKTRqvCPLDNREW 93
gi 955174305  71 DTCAICRNHIMDLCIECQAnqqs-gpaeECTVAWGTCNHAFHFHCISRWLKTRqvCPLDNKEW 132
gi 122112251  39 DTCAICRNHVMDLCIECQAssng--prtNCNIAWGVCNHAFHTHCISRWLKTRnvCPLDNKEW 99
gi 37537999   55 DNCAICRNHIMNLCIECQAdpna--nqdECTVAWGECNHAFHYHCIARWLKTRlvCPLDNKEW 115
gi 37537921   53 DNCAICRNHIMEPCIECQPkamt-dtdnECVAAWGVCNHAFHLHCINKWIKTRdaCPLDNQPW 114
gi 124426661  27 DNCAICKNHIMEKCIECDAqeg----qgECIVAWGTCNHAYHFHCIERWLKNRqtCPLDNRNW 85
gi 74664250   25 ETCAICRNHIMDTCVECQNgmt---nngECKVSWGVCNHAFHTHCITRWLSSKnvCPLDTKKW 84
gi 56474670   27 DTCAICRNSLMELCLECQGntgs--tteECTVSWGTCNHAFHTHCISSWLRQRavCPLDLKQW 87
gi 925686024  57 DTCAICRNNLYEPSIEYQAnptgdpdhpGLSIAWGVCGHVFHLDCIQRWLKTRsaCPLCNKEW 119

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