1RMD


Conserved Protein Domain Family
RING-HC_RAG1

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cd16530: RING-HC_RAG1 
Click on image for an interactive view with Cn3D
RING finger, HC subclass, found in recombination activating gene-1 (RAG-1) and similar proteins
RAG-1, also known as V(D)J recombination-activating protein 1, RING finger protein 74 (RNF74), or endonuclease RAG1, is the catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. RAG1 is the lymphoid-specific factor that mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. It also functions as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3, which is required for the joining step of V(D)J recombination. RAG-1 contains an N-terminal C3HC4-type RING-HC finger that mediates monoubiquitylation of Histone H3, an adjacent C2H2-type zinc finger, and a nonamer binding (NBD) DNA-binding domain.
Statistics
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PSSM-Id: 319444
View PSSM: cd16530
Aligned: 8 rows
Threshold Bit Score: 77.4815
Threshold Setting Gi: 157833702
Created: 2-May-2013
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
Zn binding siteRING-HC finger
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Structure:1RMD; Mus musculus RAG1 binds four Zn2+ ions through a novel zinc binuclear cluster.
    View structure with Cn3D
  • Citation:PMID 9228952

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
Feature 1             #  # #         # #  #  #           #  #   
1RMD_A         22 KSISCQICEHILADPVETSCKHLFCRICILRCLKVMGsYCPSCRYP 67
gi 2501723    290 KSVSCLVCEHILSDPVQTSCKHLFCRICILKYIKLMGcYCPSCKYP 335
gi 131826     285 KSISCQICEHILADPVETTCRHLFCRTCILSCIRVMGcYCPSCWYP 330
gi 25091093   292 RAVTCQVCDHLLSDPVQSPCRHLFCRLCIIRYTHALGpNCPTCNQH 337
gi 239785841  136 KSISCQVCEHILADPVETTCRHLFCRVCILKCLKVMGsFCPACRYP 181
gi 45737850   207 KSMSCQVCEHILADPVQSPCKHLLCRTCIAKCSRVLGnYCPTCKHP 252
gi 342672249  156 QSVSCQLCDHLLADPVQLPCMHLSCRTCILKCLAVLGkHCPVCRFP 201
gi 313104166  289 KSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGsYCPSCRYP 334

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