Conserved Protein Domain Family
RING-HC_RBR_HEL2_like

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cd16625: RING-HC_RBR_HEL2_like 
RING finger, HC subclass, found in Saccharomyces cerevisiae histone E3 ligase 2 (HEL2) and similar proteins
HEL2 is an E3 ubiquitin-protein ligase that interacts with the E2 ubiquitin-conjugating enzyme UBC4 and histones H3 and H4. It plays an important role in regulating histone protein levels and also likely to contribute to the maintenance of genomic stability in the budding yeast. HEL2 can be phosphorylated by the DNA damage checkpoint kinase and histone protein regulator Rad53. This family also includes Schizosaccharomyces pombe histone E3 ligase 1 (HEL1), also known as DNA-break-localizing protein 4 (dbl4), and Dictyostelium discoideum Ariadne-like ubiquitin ligase (RbrA). RbrA may act as an E3 ubiquitin-protein ligase that appears to be required for normal cell-type proportioning and cell sorting during multicellular development, and is also necessary for spore cell viability. Members in this family contain a RBR domain that was previously known as RING-BetweenRING-RING domain or TRIAD [two RING fingers and a DRIL (double RING finger linked)] domain. Based on current understanding of the structural biology of RBR ligases, the nomenclature of RBR has been corrected as RING-BRcat (benign-catalytic)-Rcat (required-for-catalysis) recently. The RBR (RING1-BRcat-Rcat) domain use an auto-inhibitory mechanism to modulate ubiquitination activity, as well as a hybrid mechanism that combines aspects from both RING and HECT E3 ligase function to facilitate the ubiquitination reaction. This family corresponds to the RING domain, a C3HC4-type RING-HC finger required for RBR-mediated ubiquitination.
Statistics
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PSSM-Id: 319539
View PSSM: cd16625
Aligned: 7 rows
Threshold Bit Score: 72.1296
Threshold Setting Gi: 313241367
Created: 17-Feb-2016
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Zn binding siteRING-HC finger
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Comment:Based on the structural evidence that Homo sapiens HHARI (4KBL) binds two Zn2+ ions through its RING-HC finger.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
Feature 1          #  #              # #  #  #                   #    # 
gi 549769    177 FTCIICCDKkd--tETFALECGHEYCINCYRHYIKDKLHEGn---iITCMD--CS 224
gi 157070612 138 FMCDICCEDgd-glESFAIKCGHRYCVDCYRQYLSQKIREEgeaarIQCPAdgCN 191
gi 968582996 132 FVCEICCEDek-nlQTYALECGHRYCIHCYEHYLVQKIKEEgescrIYCPGekCN 185
gi 313241367 116 FDCEICMLEfpvqeSIGTTNCDHKFCKRCYLYYIRDKINCGcs--lLRCPAhkCL 168
gi 74698267  129 GTCEICYDEgc--lPFFSAECDHEFCLACYRQYLDSRISEGes--vIQCPEesCT 179
gi 75008207  137 VSCLICLEDyp-ptQTFALICNHRYCLPCYKNYLEIKVSEGpeciyTPCPApkCK 190
gi 342319983 504 FVCDVCYDDet--kETLALTCDHRFCKACYCHYLTSKIIDEgesrrIECMGkdCH 556

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