2BAY,1N87,3JB9


Conserved Protein Domain Family
RING-Ubox_PRP19

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cd16656: RING-Ubox_PRP19 
Click on image for an interactive view with Cn3D
U-box domain, a modified RING finger, found in pre-mRNA-processing factor 19 (Prp19) and similar proteins
Prp19, also known as nuclear matrix protein 200 (NMP200), senescence evasion factor (SNEV), or DNA repair protein Pso4 (psoralen-sensitive mutant 4), is a ubiquitously expressed multifunctional E3 ubiquitin ligase with pleiotropic activities in DNA damage signaling, repair, and replicative senescence. It functions as a critical component of DNA repair and DNA damage checkpoint complexes. It senses DNA damage, binds double-stranded DNA in a sequence-independent manner, facilitates processing of damaged DNA, promotes DNA end joining, regulates replication protein A (RPA2) phosphorylation and ubiquitination at damaged DNA, and regulates RNA splicing and mitotic spindle formation in its integral capacity as a scaffold for a multimeric core complex. Prp19 contains an N-terminal E3 ubiquitin ligase U-box domain with E2 recruitment function that facilitates dimerization and is essential for its auto-ubiquitination activity in vitro or when overexpressed, a coiled-coil Prp19 homology region that mediates its tetramerization and interaction with CDC5L and SPF27, and a C-terminal seven-bladed WD40 beta-propeller type of leucine-rich architectural repeats that form an asymmetrical barrel-shaped structure important for substrate recognition and recruitment.
Statistics
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PSSM-Id: 319570
View PSSM: cd16656
Aligned: 44 rows
Threshold Bit Score: 79.5466
Threshold Setting Gi: 966656386
Created: 14-Apr-2015
Updated: 18-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
 
dimer interfaceU-box domain, a
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interface [polypeptide binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
Feature 1                   # #   ## #                              ####
2BAY_A         5 LCAISGKVPRrPVLSpkSRTIFEKSLLEQYVKDt-GNDPITNEPLs-iEEIVEIV 57
gi 75332078    2 NCAISGEVPEePVVSkkSGLLYEKRLIQTHISDy-GKCPVTGEPHt-lDDIVPIK 54
gi 124504835   4 ICTISGQTPEePVISk-TGYIFEKRLIEKHIINy-GICPVSGEVLt-lEDLYPIK 55
gi 157070380   2 LCALSGEVPEePVVSrkTGAVFEKRLIEKYIQEn-HKEPGTDEELd-vEDLLPIK 54
gi 162684884   2 FCSISGVTPEdPVVSrkSGLLFERRLILKHIADn-GTDPVTGEVLs-aEDLISIK 54
gi 239897373   4 VCAISGEVPDdPVLSk-TGYVFSRRLIEKALTEngGKCPVTGQDLdkeTDLYPIH 57
gi 288558844   2 NCAISGEVPVePVVStkSGLLFERRLIERHISDy-GKCPVTGEPLt-iDDIVPIK 54
gi 294460827   2 FCAISGSTPEePVVSkrSGLLFEKRLVEKHIAEy-GTCPVTGESLs-tDDLVPVK 54
gi 300164886   2 FCAISGLTPEePVISkrSGLLFERRLIEKHLAEn-GTCPVTGESLt-eEDLLPVK 54
gi 672572285   5 VCAISGVIPEePVFSvkTGLIYEKRLIKKHLETs-GVCPVTAQSLn-eADLADVK 57

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