Conserved Protein Domain Family
RING-Ubox1_NOSIP

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cd16661: RING-Ubox1_NOSIP 
U-box domain 1, a modified RING finger, found in nitric oxide synthase-interacting protein (NOSIP) and similar proteins
NOSIP, also known as endothelial NO synthase (eNOS)-interacting protein, p33RUL, is an E3 ubiquitin-protein ligase implicated in the control of airway and vascular diameter, mucosal secretion, NO synthesis in ciliated epithelium, and, therefore, of mucociliary and bronchial function. The loss of NOSIP may cause holoprosencephaly and facial anomalies, including cleft lip/palate, cyclopia, and facial midline clefting. NOSIP interacts with neuronal nitric oxide synthase (nNOS) and eNOS by inhibiting the nitric oxide (NO) production. It acts as a novel type of modulator that promotes translocation of eNOS from the plasma membrane to intracellular sites, thereby uncoupling eNOS from plasma membrane caveolae and inhibiting NO synthesis. NOSIP also interacts with protein phosphatase PP2A and mediates the monoubiquitination of the PP2A catalytic subunit. Thus, it is a critical modulator of brain and craniofacial development in mice and a candidate gene for holoprosencephaly in humans. Moreover, NOSIP associates with the erythropoietin (Epo) receptor (EpoR), mediates ubiquitination of EpoR, and plays an essential role in erythropoietin-induced proliferation. NOSIP contains an atypical N-terminal RING-like U-box domain that is split into two parts by an interjacent stretch of 104 amino acid residues, as well as a C-terminal RING-like U-box domain. This family corresponds to the first U-box domain.
Statistics
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PSSM-Id: 319575
View PSSM: cd16661
Aligned: 25 rows
Threshold Bit Score: 65.8283
Threshold Setting Gi: 633911158
Created: 13-Apr-2015
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
U-box domain, a
Feature 1:U-box domain, a modified RING finger [structural motif]
Evidence:
  • Comment:The U-box is a modified form of the RING finger domain that lacks metal chelating cysteines and histidines. Like the RING finger, the U-box is thought to form a structure consisting of three beta-sheets and a single alpha-helix, which would be stabilized by salt bridges instead of chelated metal ions.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
Feature 1          #  #           # #  #  #                                                      
gi 74735248   44 DCCCLSLQPChDPVVTPDGYLYEREAILEYILHQKkeiarqmkayekqrgtrreeqkelqraasqdhvrgflekesaivs 123
gi 167520936  44 DCCCLTLQPVvDGVVTPDGYLYEREAILENLLHQKreyvrqmkeyqrqverekqegleteaaahrdrieafrqaergita 123
gi 158272242  44 DDCCLTLMEAkDPVITPDGYMYSKEAILENLLAQKkaikrnmeayeaaqvadqqkelekqaiaheaalvafdrrnhlgvs 123
gi 633911158  42 YDCRLTLRRAvDPVCTPLGYIYSREAILENLLAQKkankrklaaweaqqatdaakaedraeiareaellafdrvnhqgas 121
gi 82196722   44 DCCSLSLQPCrDPVLTEDGYLYEKEAILQYILHQKteiakkmkayekqkqalksegqleskseereraekfkqrennivs 123
gi 255074353  45 DACGLGLQHAlDPVVTPEGVLYDREHILQCLLHQKkdiarkmktwegeqardaekasaeeeakrlaelekfelenkggfv 124
gi 82185586   44 DCCCLSLQPCkDPVVTSDGYIYEKESILEYILHQKkeiarqmkayekqknskkaemeelnkaakeskmkafldkemtivs 123
gi 591352960  44 DCCCLSLQPCkDPVVTPDGYLYEKEAILEYILHQKkeiarqmkleelsraaedakvkgflakemsivskplnpfdhksgt 123
gi 556961333  44 DCCSLSLQPCkDPVVTPEGYLYEKEAILEYILHQKreiakkmkafekqknekkaeaqelvkaakdtkvkaflakettivs 123
gi 353232121  44 DCCSLCLQPAkDPVVTNDGFLYDRAVVLEYIVSQKaeiqrklklyekqkarldaetkmilkaekeeearrflsmntldth 123
                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
Feature 1                                                                          #  #    
gi 74735248  124 rplnpfta-----kalsgtspddvqpgpsvgppskdkdkvlpsfwipsltpeakatklekpsrtvtCPMSGKPL 192
gi 167520936 124 aqissftkddkvrthasdaldeaksrafriktakgeqeanhaafwvpqmapeasktkltkpdkktrCPTSGRPL 197
gi 158272242 124 egvaskleaaivkeaealrnpggggaksvmaikeaedrakglkafwmpsltpdagnkvekpdsetrCPASGNKL 197
gi 633911158 122 aeladrltaa-iqeevdaalsdkkvtasainiqdhadrikelkafwvpsasseaetklekpsmqtlCPASGQKL 194
gi 82196722  124 kpinpftsgk--skdegnqngstsssstdtssgesssssalpsfwipsltpeakptllkkpsktvsCPMSGRPL 195
gi 255074353 125 nysasagts----tatgtsdariassadvaatqddkermktmkafwlpsktptaerkaekpdtdtkCPTTLKKL 194
gi 82185586  124 kplnpft--------rksdsgidaaepscsqqsseeknkqlpsfwipsltpeaktslvkkpdktvyCPMSGRPL 189
gi 591352960 124 gqqgwk---------epahppthtlqlsqsilggaeasppatppprwhlppghfytgchlpdrcvyCPMSGRPL 188
gi 556961333 124 kplnp------------ftvkadaaqpstseegadpqgkqlpsfwipsltpeakaskirkpdkcvyCPMSGMPL 185
gi 353232121 124 skasa------------qaaeaealaslsnkrnglnettscfwasgsktneklnkelldkpdtvvrCPMSGKPL 185

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