Conserved Protein Domain Family
RING-HC_MID2

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cd16754: RING-HC_MID2 
RING finger, HC subclass, found in midline-2 (MID2) and similar proteins
MID2, also known as midin-2, midline defect 2, RING finger protein 60 (RNF60), or tripartite motif-containing protein 1 (TRIM1), is a probable E3 ubiquitin-protein ligase that is highly related to MID1 that associate with cytoplasmic microtubules along their length and throughout the cell cycle. Like MID1, MID2 associates with the microtubule network and may at least partially compensate for the loss of MID1. Both MID1 and MID2 interacts with Alpha 4, which is a regulatory subunit of PP2-type phosphatases, such as PP2A, and an integral component of the rapamycin-sensitive signaling pathway. MID2 can also substitute for MID1 to control exocytosis of lytic granules in cytotoxic T cells. Loss-of-function mutations in MID2 lead to the human X-linked intellectual disability (XLID). MID2 belongs to the C-I subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil region, as well as a COS (carboxy-terminal subgroup one signature) box, a fibronectin type III (FN3) domain, and a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. MID2 hetero-dimerizes in vitro with its paralog MID1.
Statistics
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PSSM-Id: 319668
View PSSM: cd16754
Aligned: 3 rows
Threshold Bit Score: 121.682
Threshold Setting Gi: 294862489
Created: 30-Jul-2013
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Zn binding siteRING-HC finger
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Comment:Based on the structural evidence that Mus musculus RNF146 (4QPL) binds two Zn2+ ions through its RING-HC finger.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
Feature 1        #  #           # #  #  #                      #  #   
gi 294862489  30 CPICLElFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPitAFQCPTCRYV 82
gi 300116149  10 CPICLElFEDPLLLPCAHSLCFSCAHRILTSGCASNEIAEPvaVFQCPTCRYV 62
gi 971391637  10 CPICLElFEDPLLLPCAHSLCFSCAHRILLSGCSSGESIEPlsAFQCPTCRYV 62

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