Conserved Protein Domain Family
mRING-HC-C4C4_RBR_RNF144B

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cd16778: mRING-HC-C4C4_RBR_RNF144B 
Modified RING finger, HC subclass (C4C4-type), found in RING finger protein 144B (RNF144B)
RNF144B, also known as PIR2, IBR domain-containing protein 2 (IBRDC2), or p53-inducible RING finger protein (p53RFP), is a transmembrane (TM) domain-containing RBR (RING1-IBR-RING2) E3 ubiquitin-protein ligase that induces p53-dependent, but caspase-independent apoptosis. It interacts with E2 ubiquitin-conjugating enzymes UbcH7 and UbcH8, but not with UbcH5. It is involved in ubiquitination and degradation of p21, a p53 downstream protein promoting growth arrest and antagonizing apoptosis, suggesting a role in switching a cell from p53-mediated growth arrest to apoptosis. Moreover, RNF144B regulates the levels of Bax, a pro-apoptotic protein from the Bcl-2 family, and protects cells from unprompted Bax activation and cell death. It also regulates epithelial homeostasis by mediating degradation of p21WAF1 and p63. RNF144B contains a RBR domain followed by a potential single-TM domain. The RBR domain was previously known as RING-BetweenRING-RING domain or TRIAD [two RING fingers and a DRIL (double RING finger linked)] domain. Based on current understanding of the structural biology of RBR ligases, the nomenclature of RBR has been corrected as RING-BRcat (benign-catalytic)-Rcat (required-for-catalysis) recently. The RBR (RING1-BRcat-Rcat) domain use an auto-inhibitory mechanism to modulate ubiquitination activity, as well as a hybrid mechanism that combines aspects from both RING and HECT E3 ligase function to facilitate the ubiquitination reaction. This family corresponds to the RING domain, a C4C4-type RING finger whose overall folding is similar to that of the C3HC4-type RING-HC finger. It is required for RBR-mediated ubiquitination.
Statistics
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PSSM-Id: 319692
View PSSM: cd16778
Aligned: 7 rows
Threshold Bit Score: 85.5935
Threshold Setting Gi: 347921090
Created: 3-May-2013
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Zn binding sitemodified
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Comment:Based on the structural evidence that Homo sapiens RNF144A (1WIM) binds two Zn2+ ions through its C4C4-type RING finger.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*..
Feature 1          #  #              # #  #  #                  #    #    
gi 57012811   28 ITCKLCLCEQSlDKMTTLQECQCIFCTACLKQYMQLAIREGcGSPITCPDMVCLNHG 84
gi 165973414  28 ILCKLCLCEHPfDKMTSLQACSCIFCTSCLKQYIQFAIREGfGSPITCPNTVCTNQG 84
gi 449279428  28 LTCKLCLCDYSmDQMTTLQECSCIFCTSCLKQYVQLAIQEGcGSPITCPDMACLNHG 84
gi 632969905  29 VTCKLCLCEYSfDRMTTLQQCDCSFCTMCMKQYVELMIREGcGSPVTCPDMACSKLG 85
gi 557015241  28 VTCKLCLCEYPlEAMTVLQECGCVFCTQCLKLYVQVSIKEKcGSPITCPDMACTKNG 84
gi 642051814  44 VYCKLCLSEHPsAATSTLHTCDCVFCTPCLQQYVQLAIREGgGSPVTCPDMACQRTG 100
gi 347921090  28 IHCKLCLSDWPeAETCTLQSCSCVFCAQCLRQYVQLAIRAGaGSAITCPDPACKNSG 84

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