1PW4,2GFP,2V8N,2CFQ,1PV7


Conserved Protein Domain Family
MFS

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cd06174: MFS (this model, PSSM-Id:119392 is obsolete and has been replaced by 349949)
Click on image for an interactive view with Cn3D
The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.
Statistics
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PSSM-Id: 119392
View PSSM: cd06174
Aligned: 440 rows
Threshold Bit Score: 39.2193
Threshold Setting Gi: 75419378
Created: 9-Jul-2008
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
 
putative
Conserved site includes 51 residues -Click on image for an interactive view with Cn3D
Feature 1:putative substrate translocation pore
Evidence:
  • Comment:based on the structure of Escherichia coli lactose permease.
  • Comment:substrates bind at the substrate translocation pore, which has access to both sides of the membrane in an alternating fashion through a conformational change of the MFS.
  • Comment:since MFS proteins facilitate the transport of many different substrates, the residues involved in substrate binding may not be strictly conserved among superfamily members.
  • Structure:1PV7_A; Escherichia coli Lactose Permease binds the substrate homolog TDG; defined at 5A contacts
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                   #  ### ##                       #  ##  #   ## ##                  
1PW4_A     30 FLGIFFGYAAYYLVRKNFALAMPYLVeqg--------fsRGDLGFALSGISIAYGFSKFIMGsvSDRSNPRv---FLPAG 98  Escherichia coli
1PV7_A      9 FWMFGLFFFFYFFIMGAYFPFFPIWLhdin------hisKSDTGIIFAAISLFSLLFQPLFGllSDKLGLRkyllWIITG 82  Escherichia coli
P76350     24 ALGSFAGAVVDWYDFLLYGITAALVFnreffpqvspamgTLAAFATFGVGFLFRPLGGVIFGhfGDRLGRKr---MLMLT 100 Escherichia coli K12
P0AEX3     24 IVGASSGNLVEWFDFYVYSFCSLYFAhiffps-gntttqLLQTAGVFAAGFLMRPIGGWLFGriADKHGRKk---SMLLS 99  Escherichia coli K12
Q5HRH0     11 VIATGIGNAMEWFDFGLYSYLAVIISknffsqvdndqlkLVFTFATFAIAFLLRPIGGIVFGiiGDKYGRKi---VLTTT 87  Staphylococcus epi...
O51798     16 FWACYSGWALDSFDMQMFSFLLPALTltw-------gltKAEVGVLGTVALVVTAIGGWGAGilSDRYGRAr---ILVLA 85  Ralstonia eutropha...
P0C0L7     27 ITAASLGNAMEWFDFGVYGFVAYALGkvffpg-adpsvqMVAALATFSVPFLIRPLGGLFFGmlGDKYGRQk---ILAIT 102 Escherichia coli K12
P37643     24 LVASLIGTAIEFFDFYIYATAAVIVFphiffpqgdptaaTLQSLATFAIAFVARPIGSAVFGhfGDRVGRKa---TLVAS 100 Escherichia coli K12
P71369     13 LIGSAVGYGMDGFDLLILGFMLSAISadl-------nltPAQGGSLVTWTLIGAVFGGILFGalSDKYGRVr---VLTWT 82  Haemophilus influe...
P94131     19 FLFAFLALLVDGADLMLLSYSLNSIKaef-------nlsTVEAGMLGSFTLAGMAIGGIFGGwaCDRFGRVr---IVVIS 88  Acinetobacter sp. ...
Feature 1                                           ##  ##  ##  #           ##  ##     #      
1PW4_A     99 LILAAAVMLfmgfvpw----atssiavMFVLLFLCGWFQGMGWPPCGRTMVhwwsqkerggIVSVWNCAHNVGGGIPPLL 174 Escherichia coli
1PV7_A     83 MLVMFAPFFififgpll---qynilvgSIVGGIYLGFCFNAGAPAVEAFIEkvsr--rsnfEFGRARMFGCVGWALGASI 157 Escherichia coli
P76350    101 VWMMGIATAligilpsfstigwwapilLVTLRAIQGFAVGGEWGGAALLSVesapknkkafYSSGVQVGYGVGLLLSTGL 180 Escherichia coli K12
P0AEX3    100 VCMMCFGSLviaclpgyetigtwapalLLLARLFQGLSVGGEYGTSATYMSevavegrkgfYASFQYVTLIGGQLLALLV 179 Escherichia coli K12
Q5HRH0     88 IILMAFSTLligvlptyneigvwapilLLLARVLQGFSTGGEYAGAMVYVAesspdrkrnsLGCGLEIGTLSGYIAASIL 167 Staphylococcus epi...
O51798     86 IIWFTLFGVlagfa--------qsyqqLLIARTLQGLGFGGEWAVGAALMAevidsrhrgkAIGFVQSGFALGWALAVVV 157 Ralstonia eutropha...
P0C0L7    103 IVIMSISTFciglipsydtigiwapilLLICKMAQGFSVGGEYTGASIFVAeyspdrkrgfMGSWLDFGSIAGFVLGAGV 182 Escherichia coli K12
P37643    101 LLTMGISTVvigllpgyatigifapllLALARFGQGLGLGGEWGGAALLATenapprkralYGSFPQLGAPIGFFFANGT 180 Escherichia coli K12
P71369     83 ILLFAVFTGlcaia--------qgywdLLIYRTIAGIGLGGEFGIGMALAAeawparhrakAASYVALGWQVGVLGAALL 154 Haemophilus influe...
P94131     89 ILTFSILTCglglt--------qsfiqFGVLRFFASLGLGSLYIACNTLMAeyvptkyrttVLGTLQAGWTVGYIVATLL 160 Acinetobacter sp. ...
Feature 1                                                                                     
1PW4_A    175 FLLGmawfndw----haalymPAFCAILVALFAFAMMRDTpqscglppieeykndypddynekaeqeltakqifmqyvlp 250 Escherichia coli
1PV7_A    158 VGIMftin---------nqfvFWLGSGCALILAVLLFFAKtdapssatvanavgan----------hsafslklalelfr 218 Escherichia coli
P76350    181 VSLIsmmttdeqflswgwripFLFSIVLVLGALWVRNGMEesaefeqqqhyqaaa------------kkripvieallrh 248 Escherichia coli K12
P0AEX3    180 VVVLqhtmeda----alrewgWRIPFALGAVLAVVALWLRrqldetsqqetral--------------keagslkglwrn 241 Escherichia coli K12
Q5HRH0    168 VFALnisltetqlnswgwripFLLGMFLGLFGLYLRRKLEespiyeneqrelqkhp----------trepigvftlirfy 237 Staphylococcus epi...
O51798    158 ATLLlawlpke----mawrvaFWSGIIPALIVLFIRRHVKdssmferarqsrap--------------raslssvfnrky 219 Ralstonia eutropha...
P0C0L7    183 VVLIstivgeanfldwgwripFFIALPLGIIGLYLRHALEetpafqqhvdkleqgdre-----glqdgpkvsfkeiatky 257 Escherichia coli K12
P37643    181 FLLLswlltdeqfmswgwrvpFIFSAVLVIIGLYVRVSLHespvfekvakakkq--------------vkiplgtlltkh 246 Escherichia coli K12
P71369    155 TPLLlphig--------wrgmFLVGIFPAFVAWFLRSHLHepeiftqkqtalstqssf------tdklrsfqllikdkat 220 Haemophilus influe...
P94131    161 AGWLipdhg--------wrvlFYVAIIPVLMAVLMHFFVPepaawqqsrlapskqte-------tvktsafklifqdkrn 225 Acinetobacter sp. ...
Feature 1                   #  ##  ##  #                 #   #   #  #   #                     
1PW4_A    251 nKLLWYIAIANVFVYLLRYGILDWSPtylkevk----hfaldkSSWAYFLYEYAGIPGTLLCgw----msDKVFRGnrg- 321 Escherichia coli
1PV7_A    219 qPKLWFLSLYVIGVSCTYDVFDQQFAnfftsffa-tgeqgtrvFGYVTTMGELLNASIMFFApl----iiNRIGGKn--- 290 Escherichia coli
P76350    249 pGAFLKIIALRLCELLTMYIVTAFALnystqnmg-lprelflnIGLLVGGLSCLTIPCFAWLa-------DRFGRRr--- 317 Escherichia coli K12
P0AEX3    242 rRAFIMVLGFTAAGSLCFYTFTTYMQkylvnta----gmhanvASGIMTAALFVFMLIQPLIga----lsDKIGRRt--- 310 Escherichia coli K12
Q5HRH0    238 yKDIIVCFVAVAFFNVTNYMVTAYLPsylegvi----klngttTSIMITCIMVIMIPLALMFgr----iaDRIGEKk--- 306 Staphylococcus epi...
O51798    220 aRTLALSSVLVIGLQAGCYAILVWLPslln---------qrqvAAGSMIVTVFIMAFGSFCGfavtadlsDRIGRRptl- 289 Ralstonia eutropha...
P0C0L7    258 wRSLLTCIGLVIATNVTYYMLLTYMPsylshnl----hysedhGVLIIIAIMIGMLFVQPVMgl----lsDRFGRRpfvl 329 Escherichia coli K12
P37643    247 vRVTVLGTFIMLATYTLFYIMTVYSMtfstaaapvglglprneVLWMLMMAVIGFGVMVPVAgl----laDAFGRRks-- 320 Escherichia coli K12
P71369    221 sKISLGIVVLTSVQNFGYYGIMIWLPnflskql----gfsltkSGLWTAVTVCGMMAGIWIFgq----laDRIGRKp--- 289 Haemophilus influe...
P94131    226 rNMFILWALTAGFLQFGYYGVNNWMPsylesel----gmkfkeMTAYMVGTYTAMILGKILAgf----maDKLGRRf--- 294 Acinetobacter sp. ...
Feature 1                                          #  ##  #   ##  #                  ##  ##  #
1PW4_A    322 atGVFFMTLVTIATIVywmnp-----agnptVDMICMIVIGFLIYGPVMLIGLHalela-----pkkaaGTAAGFTGLFG 391 Escherichia coli
1PV7_A    291 -aLLLAGTIMSVRIIGssfa--------tsaLEVVILKTLHMFEVPFLLVGCFKyitsqf---evrfsaTIYLVCFCFFK 358 Escherichia coli
P76350    318 -vYITGTLIGTLSAFPffma-------leaqSIFWIVFFSIMLANIAHDMVVCVqqpmftemfgasyrySGAGVGYQVAS 389 Escherichia coli K12
P0AEX3    311 -sMLCFGSLAAIFTVPilsalqnvsspyaafGLVMCALLIVSFYTSISGILKAEmfpa--------qvrALGVGLSYAVA 381 Escherichia coli K12
Q5HRH0    307 -vFLIGLGGLILLSVVafslmql-qslffvsIGVLILGFFLSTYEATMPGSLPTmfyt-------hvryRALAITFNVSV 377 Staphylococcus epi...
O51798    290 ilLSVCAWIVTVSYMLlplnt------tltaILGFLVGFSAIGMFAALGPFLSElfpt--------nvrTTCMGFAYNVG 355 Ralstonia eutropha...
P0C0L7    330 lgSVALFVLAIPAFILins----------nvIGLIFAGLLMLAVILNCFTGVMAstlpam---fpthirYSALAAAFNIS 396 Escherichia coli K12
P37643    321 -mVIITTLIILFALFAfnpllgs-gnpilvfAFLLLGLSLMGLTFGPMGALLPElfpt-------evryTGASFSYNVAS 391 Escherichia coli K12
P71369    290 -sFLLFQLGAVISIVVysqltd----pdimlLAGAFLGMFVNGMLGGYGALMAEaypt--------earATAQNVLFNIG 356 Haemophilus influe...
P94131    295 -tYAFGAIGTAIFLPLivfyns----pdnilYLLVIFGFLYGIPYGVNATYMTEsfpt--------airGTAIGGAYNVG 361 Acinetobacter sp. ...
Feature 1     #  #                                         
1PW4_A    392 YLGGSVAASAIVgytv--dffgWDGGFMVMIGGSILAVILLIVVM 434 Escherichia coli
1PV7_A    359 QLAMIFMSVLAGnmye---sigFQGAYLVLGLVALGFTLISVFTL 400 Escherichia coli
P76350    390 VVGGGFTPFIAAalityfagnwHSVAIYLLAGCLISAMTALLMKD 434 Escherichia coli K12
P0AEX3    382 NAIFGGSAEYVAlslk--sigmETAFFWYVTLMAVVAFLVSLMLH 424 Escherichia coli K12
Q5HRH0    378 SLLGGTTPLIASylvs--ttnnPLTPAYYLTVISIIGFIVIALLH 420 Staphylococcus epidermidis RP62A
O51798    356 KSIGAGSVVGVGvlst--higlANAMGTFCLVAYAFAVFGIMLLP 398 Ralstonia eutropha JMP134
P0C0L7    397 VLVAGLTPTLAAwlve--ssqnLMMPAYYLMVVAVVGLITGVTMK 439 Escherichia coli K12
P37643    392 ILGASVAPYIAAwlqt---nygLGAVGLYLAAMAGLTLIALLLTH 433 Escherichia coli K12
P71369    357 RAVGGFGPVVVGsvvl---aysFQTAIALLAIIYVIDMLATIFLI 398 Haemophilus influenzae
P94131    362 RLGAAIAPATIGflas---ggsIGLGFVVMGAAYFICGVIPALFI 403 Acinetobacter sp. ADP1

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