1HUV,1AL7,1FCB,1FCB,1TB3,2J6X


Conserved Protein Domain Family
alpha_hydroxyacid_oxid_FMN

?
cd02809: alpha_hydroxyacid_oxid_FMN 
Click on image for an interactive view with Cn3D
Family of homologous FMN-dependent alpha-hydroxyacid oxidizing enzymes. This family occurs in both prokaryotes and eukaryotes. Members of this family include flavocytochrome b2 (FCB2), glycolate oxidase (GOX), lactate monooxygenase (LMO), mandelate dehydrogenase (MDH), and long chain hydroxyacid oxidase (LCHAO). In green plants, glycolate oxidase is one of the key enzymes in photorespiration where it oxidizes glycolate to glyoxylate. LMO catalyzes the oxidation of L-lactate to acetate and carbon dioxide. MDH oxidizes (S)-mandelate to phenylglyoxalate. It is an enzyme in the mandelate pathway that occurs in several strains of Pseudomonas which converts (R)-mandelate to benzoate.
Statistics
?
PSSM-Id: 239203
View PSSM: cd02809
Aligned: 151 rows
Threshold Bit Score: 224.634
Threshold Setting Gi: 58337859
Created: 20-May-2005
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1HUV_A; Pseudomonas putida MDH binds FMN and a sulfate ion
    View structure with Cn3D
  • Citation:PMID 9144771
  • Structure:1FCB_B; Saccharomyces cerevisiae Flavocytochrome b2 binds FMN and pyruvate
    View structure with Cn3D
  • Structure:1FCB_A; Saccharomyces cerevisiae Flavocytochrome b2 binds FMN and heme
    View structure with Cn3D
  • Citation:PMID 2329585
  • Comment:Note that LCHAO has a conserved Phe (1TB3, F23) rather than Try (1AL7, Y24) as in the other alpha hydroxy acid oxidizing enzyme family members with the exception of human/mouse
  • Structure:1AL7; Spinacia oleracea GO binds FMN and TACA (4-Carboxy-5-(1-pentyl)hexylsulfanyl-1,2,3-triazole), an inhibitor
    View structure with Cn3D
  • Citation:PMID 9144771
  • Structure:1TB3_A; Rattus norvegicus LCHAO binds FMN and acetate anion
    View structure with Cn3D
  • Comment:partially based on L-mandelate modeling

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                        #                                                              
1HUV_A        9 EDYRKLAQKrlpkMVYDYLEGGAedeYGVKHNRDVFqqwRFKPKRLvdvSRRSLQAEVLGKRQSmPLLIGPTGlnga-lw 87  Pseudomonas putida
2J6X_A       23 YDLEEEASKvvphGGFNYIAGASgdeWTKRANDRAWkhkLLYPRLAqdvEAPDTSTEILGHKIKaPFIMAPIAahgl-ah 101 Aerococcus viridans
BAB81584     16 SCRVCKICNgvacAGEVPGMGGKgtgDSFIRNVAALeeiKLNMRTIhdaKNPTTNIEIFGKNMElPLFAAPITgtml--n 93  Clostridium perf...
NP_711669   427 VCWVCKVCDgtdcASGVPGMGALgkmLTFQDNINALreySILPKYIrehTQASVETHFLGKKFRtPVMAAPMTgavt--n 504 Leptospira inter...
YP_011996    15 YCRVCPVCDgracAGEVPGMGGLgtgNAFRNNLSALaavRLNMRLVhgvSAPDTRTTLLGLDLDmPVLAAPIGgvsf--n 92  Desulfovibrio vu...
CAG35572     32 QCRVCPACNgracAGEVPGMGGLgtgSSFKSNFEALaevKFNMRLLhdvVEPDTSVTILGRKMAlPVLAAPIGgisy--n 109 Desulfotalea psy...
ABB15754     17 FCRVCPVCDgracAGEVPGMGGVgtgKAFQENLRALsryKLNLRTIhgvKEPDLSFELFGVKVSmPVFAAPITgtty--n 94  Carboxydothermus...
ZP_01353240  21 YCKACPECNgracRNQIPGPGAKgigDTAIRNYDKWkeiRLNMDTLvaaSNIDTKRTIYGKEFAyPFFAGPVGaisl--h 98  Clostridium phyt...
ZP_01360601  17 KCKVCKECNgvvcKGQVPGVGGKgsgSSFIRNWQKIqdvKINLDTIieeSPIDTSIELFGQKFTyPVFAAPIGavgl--n 94  Clostridium sp. ...
ZP_01594551  83 RCRVCPECDgvacSGEVPGMGGIdsgKAFRNNLAALakyELNMRTFheiKKPDTSLTLFGVKLSmPILSGITGgvtynmg 162 Geobacter lovley...
Feature 1                                   #                             #                     
1HUV_A       88 pkGDLALARAATka--------gIPFVLSTas----nmSIEDLARQcd---gdLWFQLYVi--hREIAQGMVLKALHtg- 149 Pseudomonas putida
2J6X_A      102 ttKEAGTARAVSef--------gTIMSISAys----gaTFEEISEGlng--gpRWFQIYMak-dDQQNRDILDEAKSdg- 165 Aerococcus viridans
BAB81584     94 mgGKVSEREYIEgvvk----gclDSGIYPMvgd---taVDLCLATNlev-ieeYNGQGIIfi-kPWKNEVVIEKIKMaek 164 Clostridium perf...
NP_711669   505 mnGAMDEFTFAAtlle----gchTSGTLAWlgd---gaSPEKYLIMlea-irkTKADAVLickpREDEGLLKERFQEsek 576 Leptospira inter...
YP_011996    93 mgGGVSEEDYIDaivrgcnerglVGCTGDGvppfihesGFAAITAAg-----gRGIPFVKpw-dGDELDQKLDKALAtg- 165 Desulfovibrio vu...
CAG35572    110 mgGAISEKDYVRaivn----gcrAKSIIGCtgd---gvPEVIHQEGfaa-ikaADGEAIPfi-kPWDDEELLAKLAGaka 180 Desulfotalea psy...
ABB15754     95 mgGALTDEEYSLavve----gslLAGTLAFtgd---gaDPTMYGSGlka-ikkVEGKGIPii-kPRAQEEIIKRIREaee 165 Carboxydothermus...
ZP_01353240  99 ygDSYNDLTYNEvlvk----acaDAGIAAFtgd---gvNPEVMKAAtdc-iklVDGVGVPtv-kPWNLNTIKEKSQLikd 169 Clostridium phyt...
ZP_01360601  95 ysPALDDFEYTKaiig----gckEAGVIGFtgd---gvKDEFYDLPlqv-vkeNNGHGIPti-kPWKKEEIIAKIKKaee 165 Clostridium sp. ...
ZP_01594551 163 lqGKVSEEEYIEgiia----gciQAGTIGFaad--gigDPLSVYQTrlqtvakYRGKAAGqi-kPRTQAEIIERIRLlea 235 Geobacter lovley...
Feature 1                 #      #                                                              
1HUV_A      150 --ytTLVLTTDVAvnGYRerdlhnrfkippfltlknfegidlgkmdkanlemqaalmsrqmdasFNWEALRWLRDlw--- 224 Pseudomonas putida
2J6X_A      166 --atAIILTADSTvsGNRdrdvknkfvypfgmpivqry------lrgtaegmslnniygaskqkISPRDIEEIAAhs--- 234 Aerococcus viridans
BAB81584    165 agafAVGVDIDAAglITLamngk-------------------------------------pvepKNLEEIKELVNst--- 204 Clostridium perf...
NP_711669   577 sgllAIGMDVDAVnfKTMtlkni-------------------------------------ssitRNVSKLAKIRSft--- 616 Leptospira inter...
YP_011996   166 --ckVLGMDVDAAglITLrkmgr-------------------------------------pvapKTAEELAAIVTkvhga 206 Desulfovibrio vu...
CAG35572    181 tgclAIGMDIDAAglITLrlqgr-------------------------------------pvsaKTPAKLAEIIKst--- 220 Desulfotalea psy...
ABB15754    166 tgaiAVGVDIDGAglLTMalkgq-------------------------------------pvspKTLEEVMEIVNst--- 205 Carboxydothermus...
ZP_01353240 170 cnafAVAMDVDAAglPFLknmqp-------------------------------------pagrKSVEELREIIQqi--- 209 Clostridium phyt...
ZP_01360601 166 ngapAVAMDIDAAglVTLallgk-------------------------------------pvgtKSIEDLKEIISst--- 205 Clostridium sp. ...
ZP_01594551 236 agapFFAIDIDSAgrASRalpgk-------------------------------------tvepKNLKQLRELANat--- 275 Geobacter lovley...
Feature 1             #                       #  #                                              
1HUV_A      225 phKLLVKGLLSAEDADRCIAEGadGVILSNHGGRQLDCAISPMEVLAQSVAKTG--KPVLIDSGFrrGSDIVKALALGAE 302 Pseudomonas putida
2J6X_A      235 glPVFVKGIQHPEDADMAIKAGasGIWVSNHGARQLYEAPGSFDTLPAIAERVNkrVPIVFDSGVrrGEHVAKALASGAD 314 Aerococcus viridans
BAB81584    205 klPFILKGIMTPDEAELAVEAGvdAIVVSNHGGRVLDQTPASCEVLPEIAARVKgkVKILVDGGVrtGVDILKMIALGAD 284 Clostridium perf...
NP_711669   617 klPFIVKGIMTPQDAQLAIDAGadCIVVSNHGGRVLDDMPGTARVLSGIRNVIGdkIQIVADGGVrsGMDVFKMIALGAD 696 Leptospira inter...
YP_011996   207 gaRFILKGIMCADDALRAAEVGvdAIVVSNHGGRVLDHTPGTAEVLPAIADAVKgrLAVLVDGGVrdGVDVFKMLALGAD 286 Desulfovibrio vu...
CAG35572    221 gmKFVLKGVMTADEAELALEAGceAIVVSDHGGRVLDHTPGTAEVLPEIAERVKgrMTIFVDGGVrtGGDVLKMLALGAD 300 Desulfotalea psy...
ABB15754    206 rlPFILKGIMTPDEAELAVQAGakAIVVSNHGGRTLDETPGAADVLPEIAARVKgkITILADGGVrsGVDVLKLLALGAD 285 Carboxydothermus...
ZP_01353240 210 nrPFIVKGVMTVKGAEKAFEAGasGILVSNHGGRVLDQCPSTAEVLEEIAKEFKgkMTIFVDGGIrsGADLFKSLALGAD 289 Clostridium phyt...
ZP_01360601 206 klPVILKGVMTVEGAKKALKAGayGIVVSNHGGRVLDHTPATIEVLPAIADAVKgrMKIFVDGGFrtGLDIFKAIALGAD 285 Clostridium sp. ...
ZP_01594551 276 kmPFIIKGIMTVDEAKQAVDVGaaGIVVSNHGGRVMDHTPGTAQVLAAIADKVKgdIVILADGGVryGADVLKMLALGAD 355 Geobacter lovley...
Feature 1            #                                          
1HUV_A      303 AVLLGRATLYGLAarGETGVDEVLTLLKADIDRTLAQIGCpdITSLSP 350 Pseudomonas putida
2J6X_A      315 VVALGRPVLFGLAlgGWQGAYSVLDYFQKDLTRVMQLTGSqnVEDLKG 362 Aerococcus viridans
BAB81584    285 CVLIGRPFITATFahGAKGVEEYVNSLKGELKSAMVLTGCnsIENIDN 332 Clostridium perfringens str. 13
NP_711669   697 TVLVGRPMAIFAVggGVAGVRFLISQYTDNLLQSMNVTGTetLKDIGM 744 Leptospira interrogans serovar Lai str. 56601
YP_011996   287 AVMIGRPFSIAAVggLAEGVASYVDTLKAQLVQAMILTGSadVAGISR 334 Desulfovibrio vulgaris subsp. vulgaris str. Hild...
CAG35572    301 AVMIGRPFSVAAVggLQAGVEKYIDQLSTELKQAMTLTGTaaAASVSP 348 Desulfotalea psychrophila LSv54
ABB15754    286 GVLIGRPIIVAAFggGAEGVKIYLEKIKKELREAMLLTGVarVTEVPG 333 Carboxydothermus hydrogenoformans Z-2901
ZP_01353240 290 AAIIARPFVTAVFggGYEGVRSYIQKIGAELIDVMEMCGVssLDEITS 337 Clostridium phytofermentans ISDg
ZP_01360601 286 AVLIGRPYAVAAYggGAEGVKVYTEKIGNELKETMIMAGChnLADIKR 333 Clostridium sp. OhILAs
ZP_01594551 356 AVLVGRPLVRGSVggGPEGVALMLKKMQGELVVAMTLTGTadVKKVSR 403 Geobacter lovleyi SZ

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap