2COD


Conserved Protein Domain Family
PH1_ARAP

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cd13253: PH1_ARAP 
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ArfGAP with RhoGAP domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain, repeat 1
ARAP proteins (also called centaurin delta) are phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating proteins that modulate actin cytoskeleton remodeling by regulating ARF and RHO family members. They bind phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding. There are 3 mammalian ARAP proteins: ARAP1, ARAP2, and ARAP3. All ARAP proteins contain a N-terminal SAM (sterile alpha motif) domain, 5 PH domains, an ArfGAP domain, 2 ankyrin domain, A RhoGap domain, and a Ras-associating domain. This hierarchy contains the first PH domain in ARAP. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.
Statistics
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PSSM-Id: 270073
View PSSM: cd13253
Aligned: 10 rows
Threshold Bit Score: 144.067
Threshold Setting Gi: 340382704
Created: 20-Apr-2012
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
2COD_A          9 VKSGWLDKLSPQGk-RMFQKRWVKFDGLSISYYNNEKEm---ySKGIIPLSA--ISTVRVQGD-----NKFEVVTTQRTF 77   human
BAD92710      364 IKAGWLDKNPPQGs-YIYQKRWVRLDTDHLRYFDSNKDa---ySKRFISVAC--ISHVAAIGD-----QKFEVITNNRTF 432  human
XP_003228417   14 IKAGWLDKNPPQGs-YIYQKRWVKLDPDYLRYFDGEKDi---ySKRFIPMSS--VTRVTSIGD-----QKFEVITNNRTF 82   green anole
XP_687312     437 ILSGWLDKLSPQGn-YVFQKRYVKFDGKNLMYFGSEKDa---yPKGVIPLAA--IQMARLAKD-----NKFEIVTSHRTF 505  zebrafish
CAC83946      289 LLSGWLDKLSPQGn-YVFQRRFVQFNGRSLMYFGSDKDp---fPKGVIPLTA--IEMTRSSKD-----NKFQVITGQRVF 357  human
XP_002598301  541 ERCGYLEKQGGKQsnKGWKKRWVVFDGSELIYFESKEKaqtslSKRIIPAFTmtGVTYPPKGDtg-kpNRFNLETRARTY 619  Florida lancelet
XP_003389858   47 TKEGYLFKKGGKRndKPYQRRWVVFDGEDLKYFKAKGEke-sdARNNVKLSE--MIDIKRVSDpd-hnSRFDLVVINRVF 122  Amphimedon qu...
XP_001626773  763 STSGYLYKQGGRQnnKGWKKRWFVFDGTSLKYYTNKDSq---eSLHIIPVTQm-KDVLVEPGEtr--kARFNLITGNRVF 836  starlet sea a...
EGW09603      356 DAVTTKNSISTKSrkRMFQKRWVKFDGLSISYYNNEREm---ySKGIIPLTA--ISTVRAQGD-----NKFEIVTTQRTF 425  Chinese hamster
EKC41657      642 EKSGYLFKQGGVKanKGWRKRWVVFTGNSLRYYDSRKSq---vSKRIVPLSCm-EKVETLVKDsdkdkFKFNLHTTNRVF 717  Pacific oyster
2COD_A         78 VFRVEKEEERNDWISILLNALKSQ 101  human
BAD92710      433 AFRAESDVERKEWMQALQQAMAEQ 456  human
XP_003228417   83 AFRAESDADRNDWIRALQKTVEDR 106  green anole
XP_687312     506 VFRADNDVQRSKWCSTLQERVKEQ 529  zebrafish
CAC83946      358 VFRTESEAQRDMWCSTLQSCLKEQ 381  human
XP_002598301  620 QFAAATNDEMNLWASSLMQAIIEM 643  Florida lancelet
XP_003389858  123 QFYADNDDEAQLWVSVLHAAISKY 146  Amphimedon queenslandica
XP_001626773  837 MLASDTMTDMNLWAQTLMAAILQY 860  starlet sea anemone
EGW09603      426 VFRVEKEGQHRSLGPKDSKVRSLK 449  Chinese hamster
EKC41657      718 QFAADSLQECTLWCSTLMECILKY 741  Pacific oyster
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