1D1C,4DB1,1BR1,4QBD,1JWY,1MMN,3MNQ,2JHR,2XEL,1YV3,2AKA,1D0X,1W9L,1W9J,1MMA,3MYH,1W9K,1W9I,1FMV,2Y0R,2Y9E,2XO8,1MMD,1G8X,1LVK,1MMG,1B7T,1DFK,1DFL,1KK7,1QVI,2EC6,2OS8,3I5G,2W4A,2MYS,1M8Q


Conserved Protein Domain Family
MYSc_class_II

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cd01377: MYSc_class_II 
Click on image for an interactive view with Cn3D
class II myosins, motor domain
Myosin motor domain in class II myosins. Class II myosins, also called conventional myosins, are the myosin type responsible for producing actomyosin contraction in metazoan muscle and non-muscle cells. Myosin II contains two heavy chains made up of the head (N-terminal) and tail (C-terminal) domains with a coiled-coil morphology that holds the two heavy chains together. Thus, myosin II has two heads. The intermediate neck domain is the region creating the angle between the head and tail. It also contains 4 light chains which bind the heavy chains in the "neck" region between the head and tail. The head domain is a molecular motor, which utilizes ATP hydrolysis to generate directed movement toward the plus end along actin filaments. Class-II myosins are regulated by phosphorylation of the myosin light chain or by binding of Ca2+. A cyclical interaction between myosin and actin provides the driving force. Upon ATP binding, the myosin head dissociates from an actin filament. ATP hydrolysis causes the head to pivot and associate with a new actin subunit. The release of Pi causes the head to pivot and move the filament (power stroke). Release of ADP completes the cycle. CyMoBase classifications were used to confirm and identify the myosins in this hierarchy.
Statistics
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PSSM-Id: 276951
Aligned: 165 rows
Threshold Bit Score: 879.104
Threshold Setting Gi: 568288659
Created: 1-Nov-2000
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 34 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Comment:the residues of the P-loop wrap around the triphosphate region of the nucleotide and the N-terminal region forms the binding pocket for the nucleotide
  • Structure:1D1C: Dictyostelium discoideum myosin S1DC (motor domain fragment) complexed with MgADP-BeFx, contacts at 4A
    View structure with Cn3D
  • Structure:1MMN: Dictyostelium discoideum myosin II binds AMPPNP, contacts at 4A
    View structure with Cn3D
  • Citation:PMID 9305951
  • Structure:1JWY: Dictyostelium discoideum myosin II binds ADP, contacts at 4A
    View structure with Cn3D
  • Structure:1QVI: Argopecten irradians myosin S1 binds MgADP.VO4, contacts at 4A
    View structure with Cn3D
  • Structure:1B7T: Argopecten irradians myosin S1 binds MgADP contacts at 4A
    View structure with Cn3D
  • Structure:1BR1: Gallus gallus smooth muscle myosin binds MgADP.Alf4, contacts at 4A
    View structure with Cn3D
  • Citation:PMID 9741621
  • Structure:4DB1: Human Myosin S1DC (beta isoform) binds Mn-AMP-PNP, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                 #########                                            
1D1C_A     100 PAVFHNLRVRynqdliYTYSGLFLVAVNPFKRIPIYTQEMVDIFkgrrrn------------------evaphIFAISDV 161  Dictyostelium di...
P05659     103 PAVLHNLKKRydadlfHTYSGLFLVVVNPYKRLPVYTPEIIDIYrgrqrd------------------kvaphIFAISDA 164  Acanthamoeba cas...
AAB48065   102 ATVLYNLRIRymediiHTYSGLFCVVINPYKFFPIYTPFIVSKYqgrrre------------------dvaphVYAIADG 163  Entamoeba histol...
AAD33718   101 ATVLHNLRLRysahviYTYSGLFLVAINPYKRFPIYTEEVANFYngrkke------------------lapphVFAVADY 162  Amoeba proteus
AAK17202    96 AGVLHNLRLRynkdviYTYSGLFLVAINPYKRFPIYSDTIIDIYkgrrrn------------------evaphIFAIADV 157  Physarum polycep...
NP_501620   97 ATVLNALKIRytsnviHTFCGLFCVVINPWRNIPIYSDEVKQLYqlqnd--------------------lpphVYSVAQN 156  roundworm
EFC39236   124 PSVLFNLKKRyesdkiYTYSGLFLVAVNPYKNLPIYTDEIIKKHngkrre------------------daephVFTVADV 185  Naegleria gruberi
EFC48085   103 PSVFYNLKYRyerdqiYTYSGLFLVAVNPYKNLPIYTDEVIKRHdgkrre------------------dvephIYTVSDV 164  Naegleria gruberi
ELP90006   112 ATVLYNLRLRymkdiiHTYSGLFCVVINPYQFFPIYTPFMINKYtgrrke------------------evaphVYAIAEG 173  Entamoeba invade...
ETN76983    30 ATVLDALKRRylqnviHTNCGLFCVVLNPWRALPIYTTDLMSTYrtgvgdmyphiytvaqsaydgilrgrcgfVVQIPGV 109  Necator americanus
Feature 1                          ########                                                    
1D1C_A     162 AYRSMLddrqnq---sllitGESGAGKTENTKKVIQYLASVagrnqa------------------ngsgvlEQQILqANP 220  Dictyostelium di...
P05659     165 AYRAMLntrqnq---smlitGESGAGKTENTKKVIQYLTAIagrae---------------------ggllEQQLLeFNP 220  Acanthamoeba cas...
AAB48065   164 AYRAMLdpaskfrnqsilitGESGAGKTENTKRVIQFLAMTagqskka-----------------gqlgelEEQLIdLNP 226  Entamoeba histol...
AAD33718   163 AYRSLRfnnedq---silitGESGAGKTENTKKVIQYLAIVageshkigq-------------agvaqgslDDRLLrANP 226  Amoeba proteus
AAK17202   158 AYRSMLgdklnq---silitGESGAGKTENTKKVIQYLTSVagrvsnd-----------------pnqvslEAQILqANP 217  Physarum polycep...
NP_501620  157 AFHGILkggrnq---silitGESGAGKTENTKKIIDFILSSsvsn-----------------------ntiGDCVVtSGV 210  roundworm
EFC39236   186 AYRQMLqnqlnq---smlvtGESGAGKTENTKKIIQYLTATagash--------------------gagklENQLLqTNP 242  Naegleria gruberi
EFC48085   165 AYRQMLqngenq---smlitGESGAGKTVNTKRVIQYLTHVagkgg--------------------sggqiEQQLImCNP 221  Naegleria gruberi
ELP90006   174 ALTQMRdlsaqfrnqsilitGESGAGKTENTKRVIQYIATIagrssa-------------------tmgdiENQLIqLNP 234  Entamoeba invade...
ETN76983   110 ILNLIVtggrdq---silitGESGAGKTENTKRIIEYILECcgsapsdsrhhhhlnnnnttngytdygssiGSDVVsSGV 186  Necator americanus
Feature 1             ###########                                                              
1D1C_A     221 ILEAFGNAKTTRNNNSSRFGKFIEIQfnn-agfISGASIQSYLLEKSRvvfqseTERNYHIFYQLLag--atAEEKKAlh 297  Dictyostelium di...
P05659     221 ILEAFGNAKTTKNNNSSRFGKFIELQfna-ggqITGANTFIYLLEKSRvtaqgaGERNFHIFYQILsk--amPEELKQkl 297  Acanthamoeba cas...
AAB48065   227 MLEAFGNAKTVKNNNSSRFGKFIEIQfsn-ggkIVGCIMSSYLLEKSRvvyqarEDRNYHIFYQLLag--atAQQKSEmh 303  Entamoeba histol...
AAD33718   227 LLEALGNATTIKNDNSSRFGKFIKITftqrdglISGGCIVSYLLEKSRvvsrgdSKRNFHIFYQILsg--mdVHQKATfg 304  Amoeba proteus
AAK17202   218 ILESFGNAKTTRNNNSSRFGKFIEVQfns-agyISGAKIQSYLLEKSRvvfqaeRERTFHIFYQLLag--atPEERKSmf 294  Physarum polycep...
NP_501620  211 LLEAMGNARTTHNSNSSRFGKFIRIEfde-nskLIGAKIECYLLEKSRvvfqsdGDRNFHIFYQMLsnyfdnPHKSFLkl 289  roundworm
EFC39236   243 LLEAFGNAKTLRNNNSSRFGKFIEVDfnv-sgyIAGCKIQHYLLETTRvtaqagDERNFHFFYQILsd--aqARNKYHlt 319  Naegleria gruberi
EFC48085   222 LLESFGNAKTLRNDNSSRFGKFIEIQfdk-qgyIGGCRIQHYLLETTRvirqalNERSFHIFYQIFsl--dsDKKKELyl 298  Naegleria gruberi
ELP90006   235 MLEAFGNAKTVKNNNSSRFGKFIEIQfqa--nkVIGCKMTMYLLEKSRivsqakEDRNYHIFYQLLag--atPQQRSEwr 310  Entamoeba invade...
ETN76983   187 LLEAFGNARTTHNNNSSRFGKFIRIEfne-hgkLQSAQIECYLLEKSRvvnqnqGNRNFHVFYQLLsna-fsDETRQQll 264  Necator americanus
Feature 1                                                                                      
1D1C_A     298 lagpesfnyln----qsgcvdikgvsdsEEFKITRQAMDIVGfsqEEQMSIFKIIAGILHLGNikfekgageg----avl 369  Dictyostelium di...
P05659     298 kltkpedyffln---qnacytvddmddaKEFDHMLKAFDILNineEERLAIFQTISAILHLGNlpfidvnset----agl 370  Acanthamoeba cas...
AAB48065   304 ldrpesykliyqnkkspqelnveymddaEEFKNTIKAMRTMNitdDEQNQIFKIVAGMLHLGNveysqgrdg-----ael 378  Entamoeba histol...
AAD33718   305 lssadqyeylr-----kgklvsrtiddvKDYNESLKAMEVLGfndEERDVIFSVVAAILHLGNvtfagedha-----iit 374  Amoeba proteus
AAK17202   295 lgppdtyhyln----qsgcfdvpgindaNDFQDTKNACKIMNiteEEQEAIFRVIAGILHLGNvnftqsygda----svi 366  Physarum polycep...
NP_501620  290 skkvneyky--------lrnddsmiddaETAKMTDEAFTRIGlseEEKIWIFQILSAVLWIGDikfgersgld---vsfv 358  roundworm
EFC39236   320 nvnsyayvsq------sgctsvpgindaMEFQETLKAMRIIGiseDEIDATCRIVASILHLGNckfvkddedl----sml 389  Naegleria gruberi
EFC48085   299 tepsdfeyinq-----sncyvvpgvddtNDLKLTLQSMKVMKmsdSEIDMIFRIVSFILHIGNvqfkdneeehaqivsnv 373  Naegleria gruberi
ELP90006   311 leapdkyrliyqnktspneimvdgmndlEEFKNTVAAMRTLGlsdNQQEEVFKIVAGMLHMGNvcyvdgkdm-----aei 385  Entamoeba invade...
ETN76983   265 ltksanqykfln---qgnvsidrdiddaADGVLTNRAMDRLGitsSEKMDVYRMLAACLLIGEikfgersgld---msyv 338  Necator americanus
Feature 1                                                                                      
1D1C_A     370 kdktalNAASTVfgvnpSVLEKALMEPRILAGRDlvaq--------hlnVEKSSSSRDALVKALYGRLFLWLVKKINNVL 441  Dictyostelium di...
P05659     371 kdevelNIAAELlgvsaAGLKAGLLSPRIKAGNEwvtr--------alnKPKAMASRDALCKALFGRLFLWIVQKINRIL 442  Acanthamoeba cas...
AAB48065   379 anpetfRKAAELfgvdaESLRKAIIQPKIKAGLEiier--------hlsKEEAEFNTTATLKAIYHRLFVFIVNKVNEVM 450  Entamoeba histol...
AAD33718   375 npeiinDKVGSLlgvdgRALCDALLHPHILAGKAttqgklperikkdstAIQAAASRDALVKALYGRTFLWVVEKINSSL 454  Amoeba proteus
AAK17202   367 qdktslNYAPSLfnitaSQLEKGLIEPRIQTGKElvst--------qltPAKAKSGRDALTKAIYHRLFLWIVKKINLVL 438  Physarum polycep...
NP_501620  359 esmqevDNIAELlemksSKLVDALTQPTIKVHDKlirk--------nqnLAKTLSSASAMAKILYERLFGWIVKRCNDAF 430  roundworm
EFC39236   390 persplNTASELlcvdpEGLAKGFMKPNIITPTDiiet--------hvnVAQAGFNRSALVKSMYNRLFDWLVRSINQSL 461  Naegleria gruberi
EFC48085   374 ksgsplDFACDIlsvpsDQLTKGFCKPRIILPGEiiem--------avdSRKANFNRNALVMSTYLRMFDWIVQKINQSM 445  Naegleria gruberi
ELP90006   386 snldelSKACELwgcepEALRKALIQPSFKTGKEviek--------kynKTEAEASTQAMLKFIYHRLFVFIVEQVNRVM 457  Entamoeba invade...
ETN76983   339 dgnaeiDAVTKLlsvksSRLVEALTQPSIKIGDKvirk--------nqnMQKSVFSAAALAKVLYERIFRWLLEKCNEAI 410  Necator americanus
Feature 1                                                               ######                 
1D1C_A     442 cq---------------------------------------------erKAYFIGVLDISGFEIFKvnSFEQLCINYTNE 476  Dictyostelium di...
P05659     443 shk--------------------------------------------dkTALWIGVLDISGFEIFQhnSFEQLCINYTNE 478  Acanthamoeba cas...
AAB48065   451 gk----------------------------------------------aGAHSIGILDIAGFEIFRenSFEQLCINLTNE 484  Entamoeba histol...
AAD33718   455 rsk--------------------------------------------klTHKFIGILDIAGFEIFKynSFEQLCINFTNE 490  Amoeba proteus
AAK17202   439 sq---------------------------------------------qnRVSFIGVLDIAGFEIFKnnSFEQLCINFTNE 473  Physarum polycep...
NP_501620  431 svddt---------------------------------------estcrLSRFIAVLDIAGFEIIEknSFEQFCINYTNE 471  roundworm
EFC39236   462 tsk--------------------------------------------ekIKNFIGVLDIAGFEIFElnSFEQLCINYTNE 497  Naegleria gruberi
EFC48085   446 nak--------------------------------------------ceIKNFIGVLDIAGFEIFElnSFEQMCINFTNE 481  Naegleria gruberi
ELP90006   458 rk----------------------------------------------dGDTSIGILDIAGFEIFRenSFEQLCINLTNE 491  Entamoeba invade...
ETN76983   411 sestnmrkrifvlelgwslhvylkrnesaaanptlwalsqkqravtgsvLSRFIGVLDIAGFEIVQknSFEQLCINYTNE 490  Necator americanus
Feature 1                                                                                      
1D1C_A     477 KLQQFFNHHMFKLEQEEYLKEKINWTFIDFglDSQATIDLIDGrqppGILALLDEQSVFPNATDNTLITKLhshfskkna 556  Dictyostelium di...
P05659     479 KLQQFFNHHMFTLEQQEYEREKIDWTFVDYgmDSQDCIDLIEKkp-mGILPLLDEQTVFPDADDTSFTKKLfqthenhrn 557  Acanthamoeba cas...
AAB48065   485 KLQQFFNNHMFNLEQLEYQNEGIPWTHQNFgdDLQPVIDLIESkipaGILAMLDEQSFVASGSDENFHANIcntfgrghp 564  Entamoeba histol...
AAD33718   491 RLQQFFNYHMFELELAEYQREGITWTITNFgiDGKATIDLIGSrp-kGILVLLDEDNNRGAEDDVAFNRKLidahknnpk 569  Amoeba proteus
AAK17202   474 KLQQFFNHHMFTLEQEEYKKERIDWTFIDFgmDSQATIELIESktppGILALLDEQSVFPNATDQTLITKLhthfgggqg 553  Physarum polycep...
NP_501620  472 KLQQFFNHFMFAKEQSDYLEEGIKWTQVNFanHLQPTIDLIEKp--mGILSFLEEECVVPNGSEKSLLEKLcsnlsndss 549  roundworm
EFC39236   498 KLQQFFNHHMFKKEQEEYLKEKIEWKFIDFglDLQPTIDLIEKp--lGILAILDQQTFMAQQSEEGLVREInknhgtkkq 575  Naegleria gruberi
EFC48085   482 KLQQFFNHHMFKKEQEEYLREDIAWNFIDFglDLQPTIDLIEKp--qGVLDILHQKCVVQNQDEESFVRDLlsknqksek 559  Naegleria gruberi
ELP90006   492 KLQQFFNNHMFNLEQEEYLREGIEWTRQNFgnDLQPVIDLIESkqkaSILSHLESQCFTNAGNDKQFYDLIcqafgghpk 571  Entamoeba invade...
ETN76983   491 KLQAFFNHFMFVREQSEYLDEGILWIQNDFahDLQPTIDIIEKp--lGLLSLLEEECVVPNGSDQSLLQKLctnlekypg 568  Necator americanus
Feature 1                                                                                      
1D1C_A     557 k------------yeeprfsktEFGVTHYAGQVMYEiqDWLEKNKdPLQQDLELCFKDssdnvvtklfnd---------- 614  Dictyostelium di...
P05659     558 -------------frrprfdanNFKIVHYAGEVEYQtsAWLEKNRdPLEDDLSNLCKKssvrfvtglfdedlmpsfkaap 624  Acanthamoeba cas...
AAB48065   565 rf---------rkpriggdgvyEFDILHYAGTVTYNakDWVKKNRdPLERDIQQVFIGkdssslisrlfritegia---- 631  Entamoeba histol...
AAD33718   570 f------------pvnpiaqttEFTIRHYAGDVTYDtsLWLQKNKdPLENDLEEVVSGsrkkilgtlfs----------- 626  Amoeba proteus
AAK17202   554 aqggkakkhpkyeeprfadkspNFGIYHYAGTVSYDvtNWLEKNKdPLQPDLEATMRDskdsfvrrlftesfedl----- 628  Physarum polycep...
NP_501620  550 fkk--------skqtqkcstirHFYVQHYAGEVHYNidGWLDKNRdNVETSVLDILSQsthpllkllfppv--------- 612  roundworm
EFC39236   576 -------------fktsrfnekEFILSHYAGDVPYNvvQWFTKNVdPLNEDCKSTMIAsgntlikslfdvpge------- 635  Naegleria gruberi
EFC48085   560 -------------lrkdkfdqkSFIVTHYAGEVKYNvrDWYSKNVdPLNDDCKMAMQKsqlpfikklfvd---------- 616  Naegleria gruberi
ELP90006   572 fr---------kprvastaghfEFDIIHYAGDVTYDgiDWVRKNKdPLEPQIKETFLAaqgkfiselfslhvaggd---- 638  Entamoeba invade...
ETN76983   569 fkk--------akqsqrclavrHFTIKHYAGCVDYNidSWVEKNRdVVENAVTGRTSPirrvdkfmthvppa-------- 632  Necator americanus
Feature 1                                                                                      
1D1C_A     615 ------------pniasrakkganFITVAAQYKEQLASLMATLEttNPHFVRCIIPNnkqlpakleDKVVLDQLRCnGVL 682  Dictyostelium di...
P05659     625 aeeekaaaggsrnrstgrgkggaqFITVAFQYKEQLAHLMSMLSstAPHFIRCIIPNlgkkpgvvsDQLVLDQLKCnGVL 704  Acanthamoeba cas...
AAB48065   632 ------gssgddmgtgkgsskaakFLTVGSNHKAQLEELMRKLKmtNPHFIRCILPNtqkkqryfvDSTVLKQLRCnGVL 705  Entamoeba histol...
AAD33718   627 -------------efalnskkgaqFITVASQYRTQLDDLLKTLQktTPHFIRCIIPNhdqtgeyltDHIVLDQLRCnGVL 693  Amoeba proteus
AAK17202   629 --------ptslaeyqrkgtrgaaFVTVAAQYKSQLSNLMSTLQatHPHFVRCILPNhqqkpgyleDACVLDQLRCnGVL 700  Physarum polycep...
NP_501620  613 -----------pvnnlktrrgtitNSTVSFLYKNQLQCLLDTLNssSAHFIRCVVSNyeklpgkidAPLVLAQLKCnGVL 681  roundworm
EFC39236   636 ----------rtkanekrsvgsarFKTVATNYKNQLKDLMGLLEstEPHFIRCIKPNnlqkpaiidDRLVLHQLKCnGVL 705  Naegleria gruberi
EFC48085   617 ------------qqssggsgagvrFQTVGNKYKKQLSDLIQLLSstEPHFIRCIKPNnlqkpgiiqAPSVLEQLKCnGVL 684  Naegleria gruberi
ELP90006   639 -------dsgvsaggankgqrgakFLTVGSNHREQLDDLMKKLRmtTPHFIRCILPNtqkrrhyfiDSTVLKQLRCnGVL 711  Entamoeba invade...
ETN76983   633 -----------ltrrdlltgkrqpPGCWLVICENQLTSLLETLSssSAHFIRCVVPNyeripnkidGPLVLNQLRCnGVF 701  Necator americanus
Feature 1                                                                                 
1D1C_A     683 EGIRITRkGFPNRIIYADFVKRYYLLapnvprdaedsqka----------tdavlkhlnidpeQYRFGITKIFFR 747  Dictyostelium discoideum
P05659     705 EGIRIARkGWPNRLKYDEFLKRYFLLkpgatptspstkdavkd----liehliakeptkvnkdEVRFGVTKIFFR 775  Acanthamoeba castellanii
AAB48065   706 EGIRITRmGYPNRMIYSEFLKRYYFLgdkinrrasdekatastlmrqiiekykkdgqprakegDYRFCKTKIFFR 780  Entamoeba histolytica
AAD33718   694 EGIKISRkGYPNRQKFAEFLKMYYLLgegvlkksadpktacdkimktlvkdgvvqekdadgnpTFQLGISKIFFR 768  Amoeba proteus
AAK17202   701 EGIRITRlGFPNRTIYSEFVKRYYLLvpdvprnpqdpkpa----------tatilkglkipesEYRFGLTKVFFR 765  Physarum polycephalum
NP_501620  682 EGIRICReGYPSRLSHSEFIERYSLLmknkeqskgaseke---------kctlicqdaqvrkeRYAVGKTKLFCK 747  roundworm
EFC39236   706 EGIRIARkGYPGRIPYAEFVKRYDLLvedkrsleqqpnlrg-------kaqvildsikfeettQYKLGATKVFLK 773  Naegleria gruberi
EFC48085   685 EGIRISRkGYPGRIKFNEFVKRYELLakdkksldgmklere-------kchaiiskitslddsKYKLGKTKIFLK 752  Naegleria gruberi
ELP90006   712 EGIRITRlGYPNRIIYNEFLKRYFFLgtninkktsdampvvkelmkqicekykkdgkprvnteEFRFGKTKIFFK 786  Entamoeba invadens IP1
ETN76983   702 EGIRICReGYPSRLPFAEFVTRYQILnknaslngrgae--------------eicktagidstRYQIGNTKIFCK 762  Necator americanus

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