1WGM,2QIZ,2QJ0,3M62,3M63


Conserved Protein Domain Family
RING-Ubox_UBE4A

?
cd16657: RING-Ubox_UBE4A (this model, PSSM-Id:319571 is obsolete and has been replaced by 438319)
Click on image for an interactive view with Cn3D
U-box domain, a modified RING finger, found in ubiquitin conjugation factor E4 A (UBE4A) and similar proteins
The family includes yeast ubiquitin fusion degradation protein 2 (UFD2p) and its mammalian homolog, UBE4A. Yeast UFD2p, also known as ubiquitin conjugation factor E4 or UB fusion protein 2, is a polyubiquitin chain conjugation factor (E4) in the ubiquitin fusion degradation (UFD) pathway which catalyzes elongation of the ubiquitin chain through Lys48 linkage. It binds to substrates conjugated with one to three ubiquitin molecules and catalyzes the addition of further ubiquitin moieties in the presence of ubiquitin-activating enzyme (E1), ubiquitin-conjugating enzyme (E2) and ubiquitin ligase (E3), yielding multiubiquitylated substrates that are targets for the 26S proteasome. UFD2p is implicated in cell survival under stress conditions and is essential for homoeostasis of unsaturated fatty acids. It interacts with UBL-UBA proteins Rad23 and Dsk2, which are involved in the endoplasmic reticulum-associated degradation, ubiquitin fusion degradation, and OLE-1 gene induction pathway. UBE4A is a U-box-type ubiquitin-protein ligase that is located in common neuroblastoma deletion regions and may be subject to mutations in tumors. It may have a specific role in different biochemical processes other than ubiquitination, including growth or differentiation. Members in this family contain an N-terminal ubiquitin elongating factor core and a RING-like U-box domain at the C-terminus.
Statistics
?
PSSM-Id: 319571
Aligned: 36 rows
Threshold Bit Score: 120.022
Created: 2-May-2016
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
U-box domain, a
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:U-box domain, a modified RING finger [structural motif]
Evidence:
  • Comment:The U-box is a modified form of the RING finger domain that lacks metal chelating cysteines and histidines. Like the RING finger, the U-box is thought to form a structure consisting of three beta-sheets and a single alpha-helix, which would be stabilized by salt bridges instead of chelated metal ions.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1             #  #            # #  #  #          #  #                           
1WGM_A         21 DEFLDPIMSTLMcDPVVLPSSRVTVDRSTIARHLLSDqTDPFNRSPLTmdQIRPNtELKEKIQRWLAERK 90   human
2QIZ_A        904 DEFLDPLMYTIMkDPVILPASKMNIDRSTIKAHLLSDsTDPFNRMPLKleDVTPNeELRQKILCFKKQKK 973  baker's yeast
2QJ0_A        904 DEFLDPLXYTIXkDPVILPASKXNIDRSTIKAHLLSDsTDPFNRXPLKleDVTPNeELRQKILCFKKQKK 973  baker's yeast
3M62_A        890 DEFLDPLMYTIMkDPVILPASKMNIDRSTIKAHLLSDsTDPFNRMPLKleDVTPNeELRQKILCFKKQKK 959  baker's yeast
3M63_A        890 DEFLDPLMYTIMkDPVILPASKMNIDRSTIKAHLLSDsTDPFNRMPLKleDVTPNeELRQKILCFKKQKK 959  baker's yeast
Q9HE05        933 DYFLDPLMFTIMkDPVVLPRSGISIDRSTIKAHLLSDaTDPFNRTPLTldDVTPNdTLREEINTFLKSKR 1002 Schizosaccharomyces pom...
P54860        883 DEFLDPLMYTIMkDPVILPASKMNIDRSTIKAHLLSDsTDPFNRMPLKleDVTPNeELRQKILCFKKQKK 952  Saccharomyces cerevisia...
CCD80427     1103 DEFIDPIMGCLMeDPVKLPTSGHIVDRKTIYRHLLNDsTDPFSRQPLTmsQVEPQeNLRSAVRKWIDERR 1172 Schistosoma mansoni
XP_001186638   49 DEFIDPLTFNIMeDPVSLPTSDMNIDRSTIARHLLSDqIDPFNRKPLTmeEVRSNpDLKLQIETWKQEQK 118  purple sea urchin
KFG28527      738 AEYLDPIMADVMtDPVKLPTSNNIMDRKHIERHLMSDpSDPFNRMPLTkdELIPLpELRKEIMDFIATQQ 807  Toxoplasma gondii p89

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap