4FYP


Conserved Protein Domain Family
HAD_VSP_like

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cd01624: HAD_VSP_like 
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vegetative storage proteins and related proteins, similar to soybean VSPalpha and VSPbeta proteins; belongs to the haloacid dehalogenase-like superfamily
Soybean [Glycine max (L.) Merr.] vegetative storage protein VSPalpha and VSPbeta levels were identified as storage proteins due to their abundance and pattern of expression in plant tissues, they accumulate to almost one-half the amount of soluble leaf protein when soybean plants are continually depodded. They possess acid phosphatase activity which appears to be low compared to several other plant acid phosphatases; it increases in the leaves of depodded soybean plants, but to no more than 0.1% of the total acid phosphatase activity in these leaves. This acid phosphatase activity has maximal activity at pH 5.0 - 5.5, and can liberate Pi from different substrates such as napthyl acid phosphate, carboxyphenyl phosphate, sugar-phosphates, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, phosphoenolpyruvate, ATP, ADP, PPi, and short chain polyphosphates; they cleave phosphoenolpyruvate, ATP, ADP, PPI, and polyphosphates most efficiently. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Soybean VSPalpha and VSPbeta lack this active site aspartate, other members of this family have this aspartate and may be more active. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319765
Aligned: 5 rows
Threshold Bit Score: 188.908
Created: 12-Dec-2003
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                              #####                                                     
4FYP_A        87 KEAYFYAkglalkndtVNVWIFDLDDTLLSSipyyakygy---gtentapgAYWSWLESgest----pglPETLHLYENL 159 thale cress
NP_627579     98 KERIAGSap-----geKQAVVLDIDNTSLETd------------------fGFSYPQPAnr-------pvLEVAEYAQEH 147 Streptomyces co...
YP_003766796  34 AAPAAVSaaa----paYATWIADVTAVTTPAadylagrldvpggrtaivldIDNTSLETyysggittpavKPVLALAELA 109 Amycolatopsis m...
NP_626145     88 DRAARTAhhk----htRPAVVFDIDDTLLLSldyekkn------nygydgaTWAAYVDRadr-----pavPGSPELARYA 152 Streptomyces co...
YP_169222     26 AKKIIQAqqs----lqGNAIVLDIDETALNHyyslklag----fpqgenhtIWNELLSRtda-----ypiKATLDFYLYC 92  Francisella tul...
Feature 1                  ##                                                       #            
4FYP_A       160 lelGIEPIIISDRwkKLSEVTVENLKAVGVTk---wKHLILKpngsk--------------ltqvVYKSKVRNSLVkkg- 221 thale cress
NP_627579    148 ---GVALFFVTARpgIIEAPTEWNLAHAGYEs----SGLYVRgfldl-------------fkdvaEYKTEQRAEIEskg- 206 Streptomyces co...
YP_003766796 110 kskGAAVFFVSDRteLLRWPTEGNLKAVGYPv----DGLYLRplfnf--------------dpvqANKTKARAAIEqag- 170 Amycolatopsis m...
NP_626145    153 dkkGVEVFYNSGLgeAQRAAAVENLKKAGADvnldaSHMFLKdkanppsylahcatpgswtcttvQYKAGTRRHIErdlg 232 Streptomyces co...
YP_169222     93 ltsGLKVFFISARfaQYLESTKQALRNAGYVn---fEDVFVFpenieqy----------nskdfkNFKAERRAYIEslg- 158 Francisella tul...
Feature 1               ##  ##                 
4FYP_A       222 ynIVGNIGDQWADLVEDTPGRVFKLPNPLY 251 thale cress
NP_627579    207 ytIIANIGNSASDLSGGHAEKTFKLPDYDG 236 Streptomyces coelicolor A3(2)
YP_003766796 171 ytIVANVGNNRTDLDGGHAERTFKLPDYDG 200 Amycolatopsis mediterranei U32
NP_626145    233 ydVVANFGDQYSDLQGGYADRTYKLPNPTY 262 Streptomyces coelicolor A3(2)
YP_169222    159 ykILISIGDQSSDLLGGYTLYTLQLPNYLY 188 Francisella tularensis subsp. tularensis SCHU S4

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