Conserved Protein Domain Family
M48B_HtpX_like

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cd07327: M48B_HtpX_like 
HtpX-like membrane-bound metallopeptidase
This family contains peptidase M48 subfamily B, also known as HtpX, which consists of proteins smaller than Ste24p, with homology restricted to the C-terminal half of Ste24p. HtpX, an integral membrane (IM) metallopeptidase, is widespread in bacteria and archaea, and plays a central role in protein quality control by preventing the accumulation of misfolded proteins in the membrane. Its expression is controlled by the Cpx stress response system, which senses abnormal membrane proteins. HtpX participates in the proteolytic quality control of these misfolded proteins by undergoing self-degradation and eliminating them by collaborating with FtsH, a membrane-bound and ATP-dependent protease. HtpX contains the zinc binding motif (HEXXH), has an FtsH-like topology, and is capable of introducing endoproteolytic cleavages into SecY (also an FtsH substrate). However, HtpX does not have an ATPase activity and will only act against cytoplasmic regions of a target membrane protein. Thus, HtpX and FtsH have overlapping and/or complementary functions, which are especially important at high temperature; in E. coli and Xylella fastidiosa, HtpX is heat-inducible, while in Streptococcus gordonii it is not. Mutation studies of HtpX-like M48 metalloprotease from Leptospira interrogans (LA4131) has been shown to result in altered expression of a subset of metal toxicity and stress response genes.
Statistics
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PSSM-Id: 320686
View PSSM: cd07327
Aligned: 30 rows
Threshold Bit Score: 138.926
Threshold Setting Gi: 805374630
Created: 14-Jul-2008
Updated: 18-Aug-2016
Structure
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Aligned Rows:
 
Zn binding siteputative active
Feature 1:Zn binding site [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
O58997        47 SYWFSDSIVLnwynari------------------------vseeeapELHYIVEKLARQAGIP---KPRVAIVPTm-vP 98  Pyrococcus hori...
Q1RAV8        50 SLLMSKWMALrsvggevie---------------------qprnererWLVNTVATQARQAGIA---MPQVAIYHAp-dI 104 Escherichia col...
AKB71000      61 IYVVSLVIAIspfgewilrlhsgc-----------rkikrkdqidyiePIFKEVLKQARELDPHiseDVKLYIRNTq-dV 128 Methanosarcina ...
Q9YD67        48 VQWLFSPYIInmvyrtr------------------------eplpgeeWIVAEVEQLARRSGLK---PPKVVVSEMn-mP 99  Aeropyrum perni...
ETX01369      44 LYRWSERVVLrlheare------------------------iteaeapDCLAITRALALRAGIP---APRLYAVDTl-tP 95  Candidatus Ento...
WP_018997468  44 VFWKADTLVLrankaqevd--------------------enhpspmvrAFVKECRDLSSSAGMI---CPRLFIMDAh-qP 99  Hirschia maritima
ERI39911      50 GYWNSDKIAIramharp------------------------vseleqpAMYRIVRELSTEARQP---MPRLYVSPTq-aP 101 Bacillus sp. EG...
EFH27528      53 AYWCSSKLAIiamrate------------------------vseeeepVLYAMVRDLSARIGKP---MPRVMVSPYe-sP 104 Gardnerella vag...
CRH67233      54 SYWNSATIALrsmnayq------------------------vtreerpDLYRIVEELAARAKQP---VPSIWIAPTa-tP 105 Chlamydia trach...
KIE13353      55 IGLPSLYWVVtttyryfsavptyinqrnirvinpflldekglyskeeqWLCSTIRDLAIAEKIPk--PPVVGIELCddnA 132 Tolypothrix bou...
Feature 1                                          #   #                                         
O58997        99 NAFATGrsprnAVVAVTEGLLqllnkDELEGVIAHEISHIKNrDTLIQTIAAvlagaimilvdfarwslwfgaydder-- 176 Pyrococcus hori...
Q1RAV8       105 NAFATGarrdaSLVAVSTGLLqnmspDEAEAVIAHEISHIANgDMVTMTLIQgvvntfvifisrilaqlaagfmggnrde 184 Escherichia col...
AKB71000     129 NAFAIGr----KTICITDGLLka-pvEQIKATIAHEIGHIAHrDTDWILVISvgnfivtfvyiivrvfgkmmstffellp 203 Methanosarcina ...
Q9YD67       100 NAFAYGspiagSYVAVTRGLLrllpkDEVRAVLAHEVGHLKHrDVTVILALSlipiaafligrtlvwagilgggggerr- 178 Aeropyrum perni...
ETX01369      96 NAFAIGrssqhASLVITEGLLqrldqDEWTSVIAHEVAHIKHrDILVMTMTAalagilsrpfasvipqwpfladpipag- 174 Candidatus Ento...
WP_018997468 100 NAFAVGrdalhASIAVTNGLLmtlnrAETRAVIAHELAHVKRgDSLSMGIASavsgllnrltwiffaplg---------- 169 Hirschia maritima
ERI39911     102 NAFATGrnpknAAVCCTEGILrlldeRELRGVLGHELMHVYNrDILTSSVAGalagvitslaqfllifggdrdrg----- 176 Bacillus sp. EG...
EFH27528     105 NAFATGrserhASICCTRGLInilneRELRSVIGHELTHIYNhDILASSLATatatvltyvgswlvyvgdtyrrdir--- 181 Gardnerella vag...
CRH67233     106 NAFATGrdpnhAAVCCTEGILeilgeRELRGVLGHELMHVYNrDILTSSVAAgiagligsvaqfllffgggsrrdgeg-- 183 Chlamydia trach...
KIE13353     133 NAYAVGatrsySMVVATTGLMkhlnrEQVAAVMAHEIGHIANlDTMAKTLLSaswsgvmmvlfapmllvvwylgrfvall 212 Tolypothrix bou...
Feature 1                                                            #                           
O58997       177 ----------------------dsgsviglilaiilaplaatliqlaISRSREYLADETGARISgkphaLASALMKIeea 234 Pyrococcus hori...
Q1RAV8       185 ge---------------esngnpliyfavatvlelvfgilasiitmwFSRHREFHADAGSAKLVgr-ekMIAALQRLkts 248 Escherichia col...
AKB71000     204 ssdnltd-----ggvkvagmvqsyitdaiiialmwawtkigillvmkSNRDNEFEADKFAFNLS-----YGNALCELlds 273 Methanosarcina ...
Q9YD67       179 ---------------------gnpmalvavgaallaagmvfqlivshFNRLREYYADAHSALVTgsprsLQRALARIhaa 237 Aeropyrum perni...
ETX01369     175 --------------------skdglrtaagalasvivtplaawsrlfHSRLREFEADEASAWLTgdplaLVEALRKLdyw 234 Candidatus Ento...
WP_018997468 170 ------------------------------lsaissrfisrtvaqifASKAREHAADRDGAKICgkpldLASALEKIern 219 Hirschia maritima
ERI39911     177 ------------------------gnplaaiamvvlapiaamlvqlaISRTREYDADEDGAKLTgdplaLASALRKLdag 232 Bacillus sp. EG...
EFH27528     182 ----------------------kyckfigkflnilfapiasviinaiIPGSREFDADKGSSEITgdfaaLASALNKItyg 239 Gardnerella vag...
CRH67233     184 ----------------------ggvgvigsiliallapfaatliqfsLSRTREFDADEDGSKLTgdplaLASALRKLeqv 241 Chlamydia trach...
KIE13353     213 mlgagliyglfgswqeatgfftgviiwivlmllpsmtqflvtlltfwHSRWREYHADAVAARLTsv-daMVSALATLdrl 291 Tolypothrix bou...
Feature 1                                                                  #           
O58997       235 vryrplrrgnpat-------------------------ahmfiinpfrgidfaELFSTHPpteKRIERLR 279 Pyrococcus horikoshii OT3
Q1RAV8       249 yepqeatsmm-------------------------------afcingkskslsELFMTHPpldKRIEALR 287 Escherichia coli UTI89
AKB71000     274 stes------------------------------------------stkglfaNLMSSHPdknDRIAALM 301 Methanosarcina mazei C16
Q9YD67       238 yehnphlveearsnemasmlfivapltsltasplvdvdylverlkeqetnplvELFSTHPpvsKRLRFLD 307 Aeropyrum pernix K1
ETX01369     235 nqvtpmqvgspvm-------------------------ahlytvnpfawgkwaQGFDTHPstvDRILLLE 279 Candidatus Entotheonella ...
WP_018997468 220 tvsllnpvaeknp------------------------atahiyvvdplhaskrTHRSSHPateKRIARLR 265 Hirschia maritima
ERI39911     233 tqraplpqnrdlvd-----------------------vshlmianpfrgagvgKMFATHPpmaERIKRLE 279 Bacillus sp. EGD-AK10
EFH27528     240 mqlhemkstaglqav---------------------aslmiicpwnnnapalvRLCTARPtvqDRIGRLM 288 Gardnerella vaginalis AMD
CRH67233     242 tahrpmeqtpanq-------------------------svaammianpfgkarNLFSTHPsmdERCARLE 286 Chlamydia trachomatis
KIE13353     292 pfqppkkdreqms------------------------tlwvrvpmkeetgtfhWLKHTHPpihKRMEALR 337 Tolypothrix bouteillei Iicb1

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