Conserved Protein Domain Family
ArfGap_ARAP1

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cd17901: ArfGap_ARAP1 
ArfGap with Rho-Gap domain, ANK repeat and PH domain-containing protein 1
The ARAP subfamily includes three members, ARAP1-3, and belongs to the ADP-ribosylation factor GTPase-activating proteins (Arf GAPs) family of proteins that promotes the hydrolysis of GTP bound to Arf, thereby inactivating Arf signaling. The function of Arfs is dependent on GAPs and guanine nucleotide exchange factors (GEFs), which allow Arfs to cycle between the GDP-bound and GTP-bound forms. In addition to the Arf GAP domain, ARAPs contain the SAM (sterile-alpha motif) domain, 5 pleckstrin homology (PH) domains, the Rho-GAP domain, the Ras-association domain, and ANK repeats. ARAPs show phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3)-dependent GAP activity toward Arf6. ARAPs play important roles in endocytic trafficking, cytoskeleton reorganization in response to growth factors stimulation, and focal adhesion dynamics. ARAP1 localizes to the plasma membrane, the Golgi complex, and endosomal compartments. It displays PI(3,4,5)P3-dependent ArfGAP activity that regulates Arf-, RhoA-, and Cdc42-dependent cellular events. For example, ARAP1 inhibits the trafficking of epidermal growth factor receptor (EGFR) to the early endosome.
Statistics
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PSSM-Id: 350088
Aligned: 6 rows
Threshold Bit Score: 229.699
Created: 13-May-2012
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Zn binding sitearginine finger
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Comment:the coordination of the Zn2+ ion by 4 cysteines would indicate that this ion plays a structure stabilization rather than catalytical role.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                     #  #                #  #                                         
Q96P48     535 SEVAERIWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDrkVWTETLIELFLq-LGNGAG 613  human
CAF92925    65 CVVAEQIWAEPSNSFCADCGIPKPEWAAINLCVVICNQCAGEHRGLGPSISKVRSLKMDrkVWTEELVKVFLc-IGNERA 143  spotted green pu...
EMP27295   173 SEVAEKIWSVEANQFCADCGSPKPDWASVNLCVVICKRCAGEHRGLGPSITKVRSLKMDrkVWTEELIELFCk-VGNCVA 251  green seaturtle
OCT93425   731 LEVAQKIWSVEGNRRCSDCNGSNPDWASINLCVVMCKRCAGEHRSLGPNISKVRSLKMDhkVWTPELIELFQk-VGNAIS 809  African clawed frog
KTF93382   261 DGLCESIWRVPSNRMCADCSSSMPEWASVNLCVLLCEKCAGAHRSLGQNVSKVRSLKLDerVWTDDLIRVFLl-LGNGKA 339  common carp
CDQ62144   210 NAVAQKIWSEEANQRCADCGAPHPDWASVNLCVVLCKCCAGVHMGLGQSVSKVRSLKMDekAWTENQVGTLFllLGNDKV 289  rainbow trout
Feature 1                                           
Q96P48     614 NRFWAANVPPSEALqpsSSPSTRRCHLEAKYREGKYR 650  human
CAF92925   144 NSFWAANVPPSEALspsSCREERHHFISNKYHQGKYR 180  spotted green pufferfish
EMP27295   252 NQFWAANVPPSEAIhtaSRSQERRHFLVAKYKEGKYR 288  green seaturtle
OCT93425   810 NRFWAANVPPSEAIdvtSSTQERKKFILAKYREGKYR 846  African clawed frog
KTF93382   340 NQFWGANIPPSESLcssAHSEQRLEHITAKYKDGKYR 376  common carp
CDQ62144   290 NLFWAANIPPSEMLcpsSDREERQRFVTAKYCQGKYR 326  rainbow trout

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