Conserved Protein Domain Family
7tmB3_Methuselah-like

?
cd15039: 7tmB3_Methuselah-like 
Methuselah-like subfamily B3, member of the class B family of seven-transmembrane G protein-coupled receptors
The subfamily B3 of class B GPCRs consists of Methuselah (Mth) and its closely related proteins found in bilateria. Mth was originally identified in Drosophila as a GPCR affecting stress resistance and aging. In addition to the seven transmembrane helices, Mth contains an N-terminal extracellular domain involved in ligand binding, and a third intracellular loop (IC3) required for the specificity of G-protein coupling. Drosophila Mth mutants showed an increase in average lifespan by 35% and greater resistance to a variety of stress factors, including starvation, high temperature, and paraquat-induced oxidative toxicity. Moreover, mutations in two endogenous peptide ligands of Methuselah, Stunted A and B, showed an increased in lifespan and resistance to oxidative stress induced by dietary paraquat. These results strongly suggest that the Stunted-Methuselah system plays important roles in stress response and aging.
Statistics
?
PSSM-Id: 410632
View PSSM: cd15039
Aligned: 72 rows
Threshold Bit Score: 143.905
Threshold Setting Gi: 19922988
Created: 11-Nov-2013
Updated: 25-Oct-2021
Structure
?
Aligned Rows:
  next features
Feature 1:putative polypeptide ligand binding pocket [polypeptide binding site]
Evidence:
  • Comment:based on mutagenesis of human glucagon receptor (GCGR), and modeling studies of GCGR and other related class B GPCRs
  • Comment:Residues in the globular N-terminal extracellular domain and the extracellular loops of the 7TM domain may also be involved in ligand binding.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1            #   #                                         #  ##  #         #      #  
EFN65885  304 TNHQLTSKPVGLIISLPFLLATFVVYSILPElGNMHGYTLRGYVGSLFVSYTVLVVLQSISpndikksiCITLAFFIHFS 383 Camponotus floridanus
ELU09139    3 SVIQTAVSVIGQILSIIGLVIQLTVYSILPKlRNLAGLNLMSLSASLLVAQLLFLLGSSASsvy---elCATMAAVTHFA 79  Capitella sp. I Gr...
EFX73784   31 STGQHYMSDICLAISVVCLAFHIIVHMILPKlRNLPGKNLLSLSCALFMAQLLFLTGIGLRdtvg-yrlCATLGVLTHWF 109 common water flea
ELT99274  443 SSTQTIVSVIGQVISIVCLSLHLLVYSMFRKlRNLPGLNIMSLSACLLIAQLLFLFAGSVSvsy---swCFTIGVLVHYF 519 Capitella sp. I Gr...
ELU00377  337 DVGLGYLTLVGSALSIFGLLLLLLAYTCLPGlRNTPGKSLMSLAVSLLLAQALYASGLNLMqpr---slCVSMAMFVHFF 413 Capitella sp. I Gr...
ESO83924   84 DDVQAILSVIGITLSIIGCVLTICVYLSFKSlRNIPGKIVISLCISLILAYLTMLIGPAMRenf---agCKSFAIMMHYF 160 Lottia gigantea
EEB14446  452 GQYMGYITAVGLGVSIVFLVLHLLAFLVVPDlQNLSGKNLASLCVALLLANISFISGQRITtgt---rsCAASGIVTYYG 528 human body louse
EEC16509  422 AGIQDVLSTVVLLISVICLVLHVAAYALAPKlRTGPSRLLLCLAVSVLVAQASFVAGGMLFmpn--tklCAALGIVSHGG 499 black-legged tick
EFX79407  532 LIVESYLTLCLMSVSILAMMATIVTYLIFPElRNLAGLAVMNLCLMTSGFQLCIMIGMSLSvhqk-selCVASAILVHYE 610 common water flea
EEC15198  331 KDIGGYFTLAGSVVSLSCLLIKMCLYFFTSRvKKLPDHLILMLSVSIFAAQLLFLFILIVEnyq---tlCVAVGLLVHYF 407 black-legged tick
Feature 1                                                                                     
EFN65885  384 FLASFFWLNVMCFDIWWTFGgfrslqg-svkqrekKKFIIYSIYAWGSASILTIVCAIMDfvpnvp-kefirPEFGeatC 461 Camponotus floridanus
ELU09139   80 FLASFSWMHVMAFDIWKTFAkssqpn---sldnstKKYFRYSLYGWGVPLLITTTAITVNfsvae--daayrPAYGemiC 154 Capitella sp. I Gr...
EFX73784  110 YLAAFFWMNVMGFDICRTFTgslirhrgmqgrgqrCTFIFYSLYAWGFPTLVVSLGIMLDlthlm---ddyaPEYGtrlC 186 common water flea
ELT99274  520 FLASFLWMNVMAFDICRTFVkfskps---lrddysSKFIRYCAYCILVALVIVLSAIVANftlgd---slyrPGYGegiC 593 Capitella sp. I Gr...
ELU00377  414 FLSQFCWMNVLAFDVWRSFRmhniglh-srlrtrsRHFVFYCLYAWLLPAVVVLISFLLDlfqa-----sirPAYGqsfC 487 Capitella sp. I Gr...
ESO83924  161 FLVAFFWMNVMAIDVWYTFAyhqagg----ssersTRFRFYVLYVFIMASIPVILSTILNeteia---ddfsPHYGegiC 233 Lottia gigantea
EEB14446  529 YLASFFWMLTMAFDIWRTLKiatfelr-vssgkqwKKFIVYSIWSWMAPLILMIAAVVTDispsgtvnvyirPFFGvhsC 607 human body louse
EEC16509  500 HLAAFFWMNAMAVDIQRTFRagirgs---sragswRSFMGYSLYAWLGPVLLVASASLLDhllpq---splsPAYGrpsC 573 black-legged tick
EFX79407  611 GLASIFWTNVMAIDLFLTLGrwsaa-----prkpsKILPRYSLYAYGLPLAIVSVAVAINfcdct---gefeVEYGklfC 682 common water flea
EEC15198  408 FLASFFWTNALAFDLWRSFVsvvrtr----ktnrgKTLCAFNAYCWTMPFLVVCAAGGADfllpr---hsfsPRYGqgiC 480 black-legged tick
Feature 1            ## # #                                                              #  # 
EFN65885  462 WFntDGAKALYFYGPMSITVICNICLFISTALKILRHKKdtdhhlkg--sesrrhdDNKQWFNLYLKLFIVMGINWSMEI 539 Camponotus floridanus
ELU09139  155 WIrsRIGLAIFFATPVAAVLVFNIVFYTLTVVSIYKITKmargv--------tkpkSDQRQLWLYVKLTCIMGLTWIFGF 226 Capitella sp. I Gr...
EFX73784  187 WIsnKTGLGFLFMVPVAVLLLENMILFSLTVFSILKQRRaarfavdkkqsyrkageSKSIRFILYIKLGLIMGMGWIFGF 266 common water flea
ELT99274  594 WIknRKALLAFFAAPVAVIVTSNLVYFFITLWSIQKSMEaskma-------agnkkGERDRFWLYVKLSTIMGLTWVFGF 666 Capitella sp. I Gr...
ELU00377  488 WLgsRRGLLVLFASPISIMLIFNCGLFSLTARSLCIAGEdsdll-------nlsrrEHRRRLLLYVKLAVVMGLAWVFGF 560 Capitella sp. I Gr...
ESO83924  234 WFtrRNALLVFFGLPSLLVLIANVILFLIAANTIYSAQKnsvr---------llgkEDKSMLSIYVRLTIVMGVAYLFGF 304 Lottia gigantea
EEB14446  608 WFghRQALLIFFAAPFAVIMIANIGFFASSAHMIFSTTSttry---------tasgSTQRDFRLYIRLALVMGLTWLTGL 678 human body louse
EEC16509  574 WLnrPLSLAAFFGAPVLLLLLTNLVLFALTAHSIQRTEQqtkvah-----rqsrrlGDQVRFVLYVKLAVMFGLTWVFGF 648 black-legged tick
EFX79407  683 WIsnPTANMVFFGLPLALALVANIVLFVRTVAIIYSSSAcqgkq-------csrrqRALCQLKLYARMSTVMGFAWIFAF 755 common water flea
EEC15198  481 WIsnRMALLVFFAAPIASIIFSNMALFAMSVVRISGTMSrtdq---------arhrASKGRLLIYFKLALVMGMTWTFGF 551 black-legged tick
Feature 1                    #   #                              
EFN65885  540 VSWLFkn---ePKYLWYLTDLTNTLQGLIIFIIFVCKK-KVKRLLLKRFG 585 Camponotus floridanus
ELU09139  227 VAAAFd-----LEVLWYLFIVVNSLQGAFICVAFVCTR-NVVKLLREKFS 270 Capitella sp. I Grassle & Grassle, 1976
EFX73784  267 IAALAk-----IPALWYPFIFFNALQGAFIFVAFCCKR-KIYFMLYQWAT 310 common water flea
ELT99274  667 VAAIFd-----VEVLWYLFIIFNSLQGAMICFAFVMTK-QVLSLLRGKFS 710 Capitella sp. I Grassle & Grassle, 1976
ELU00377  561 LATAFd-----SKILWYIFTALNTFQGLFICISFVFTQ-KVLRLLRKKFT 604 Capitella sp. I Grassle & Grassle, 1976
ESO83924  305 LVPLYn-----HPAILYIFIILNTLQGLFIFVSFVCTR-KVLGLIRERAN 348 Lottia gigantea
EEB14446  679 FAGYLd-----IDALWYSFIALNTLQGLFIFLAFTCND-KVIRTIKELIC 722 human body louse
EEC16509  649 AAAISg-----ISFLWFPFIVLCGLHGAFIFLAFTFKK-SVANMVLETLK 692 black-legged tick
EFX79407  756 IAACFdpsstaGMIFTFVYVILNSMGGLFIFVAFTCNR-RVYLLYRGWWA 804 common water flea
EEC15198  552 VAPFAn-----IQALWYVFVILNSLQGAFVLFAFTKLEvRANLETFATWL 596 black-legged tick

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap