2EO5,1BJO,1H0C,1WYU,1BJ4,1JS6,1JG8,1LW4,2TPL,1ORD,2E7J,1MDZ,1FC4,1N8P,1AJS,1OHV


Conserved Protein Domain Family
AAT_I
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cd01494: AAT_I 
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Aspartate aminotransferase (AAT) superfamily (fold type I) of pyridoxal phosphate (PLP)-dependent enzymes. PLP combines with an alpha-amino acid to form a compound called a Schiff base or aldimine intermediate, which depending on the reaction, is the substrate in four kinds of reactions (1) transamination (movement of amino groups), (2) racemization (redistribution of enantiomers), (3) decarboxylation (removing COOH groups), and (4) various side-chain reactions depending on the enzyme involved. Pyridoxal phosphate (PLP) dependent enzymes were previously classified into alpha, beta and gamma classes, based on the chemical characteristics (carbon atom involved) of the reaction they catalyzed. The availability of several structures allowed a comprehensive analysis of the evolutionary classification of PLP dependent enzymes, and it was found that the functional classification did not always agree with the evolutionary history of these enzymes. Structure and sequence analysis has revealed that the PLP dependent enzymes can be classified into four major groups of different evolutionary origin: aspartate aminotransferase superfamily (fold type I), tryptophan synthase beta superfamily (fold type II), alanine racemase superfamily (fold type III), and D-amino acid superfamily (fold type IV) and Glycogen phophorylase family (fold type V).
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Statistics
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PSSM-Id: 99742
Aligned: 20 rows
Threshold Bit Score: 88.9787
Created: 6-Mar-2002
Updated: 17-Jan-2013
Structure
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Program:
Drawing:
Aligned Rows:
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catalyticpyridoxal
Conserved site include 1 residue -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:pyridoxal 5'-phosphate (PLP) forms an internal aldimine bond (Schiff base linkage) with the catalytic residue lysine
  • Comment:the catalytic lysine in most cases is part of a flexible region
  • Structure:2EO5_A; 4-aminobutyrate-aminotransferase, catalytic residue (lys) and cofactor (PLP)
    - View structure with Cn3D
  • Structure:1BJ4_A; serine hydroxymethyltransferase, catalytic residue (lys) and cofactor (PLP)
    - View structure with Cn3D
  • Citation:PMID 7748903
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
2EO5_A     87 PQLELAKKLvtysp--------gnfqkKVFFSn-sGTEAIEASIKVVknt------------------grkYIIAFlggf 139 Sulfolobus tokodai...
1H0C_A     56 IMDEIKEGIqyvfq---------trnpLTLVIsgsGHCALEAALVNVlep-------------------gdSFLVGangi 107 human
1WYU_B    108 LMWELGEYLkalt-----------gmdAITLEpaaGAHGELTGILIIrayhedr----------gegrtrrVVLVPdsah 166 Thermus thermophilus
1BJ4_A     82 LETLCQKRAlqaykl-------dpqcwGVNVQpysGSPANFAVYTALvep-------------------hgRIMGLdlpd 135 human
1JS6_A    114 LETVMMDWLgkmlqlpeaflageagegGGVIQg-sASEATLVALLAArtkvvrrlqaaspgltqgavleklVAYASdqah 192 pig
2TPL_B     76 NFYHLERTVqelf-----------gfkHIVPTh-qGRGAENLLSQLAikp-------------------gqYVAGNmyft 124 Citrobacter freundii
NP_354805  70 AYEAAREKVrrfln--------agsvdEIVFTk-sSTEAINTVAYGYgmpvi---------------gegdEIVISimeh 125 Agrobacterium tume...
2E7J_A     54 PIHDFIHNQlpkf----------lgcdVARVTn-gAREAKFAVMHSLakk-------------------daWVVMDench 103 Archaeoglobus fulg...
1N8P_A     56 NRENLERAVaale-----------naqYGLAFs-sGSATTATILQSLpq--------------------gsHAVSIgdvy 103 Saccharomyces cere...
1AJS_A     75 GLAEFRTCAsrlalgdds-palqekrvGGVQSl-gGTGALRIGAEFLarwyng------------tnnkdtPVYVSsptw 140 pig

Feature 1                                                                                     
2EO5_A    140 hgrtfgsisltaskavqrsivgpfmpGVIHVpypnpyrnpwhingyenpselvnrviefiedyifvnlvppeeVAGIFFE 219 Sulfolobus tokodai...
1H0C_A    108 wgqra------------vdigerigaRVHPMtkdpgghytlq---------------------eveeglaqhkPVLLFLT 154 human
1WYU_B    167 gsnp--------------atasmagyQVREIpsgpegevdl----------------------ealkrelgphVAALMLT 210 Thermus thermophilus
1BJ4_A    136 gghlthgfm----tdkkkisatsiffESMPYkvnpdtgyiny--------------------dqleenarlfhPKLIIAG 191 human
1JS6_A    193 ssve--------------ragliggvKLKAIpsdgkfamrasal----------------qealerdkaagliPFFVVAT 242 pig
2TPL_B    125 ttry---------------hqekngaVFVDIvrdeahdaglniafkgd--------idlkklqklidekgaenIAYICLA 181 Citrobacter freundii
NP_354805 126 hsnivp----------whfirerqgaKLVWVpvdddgafhie---------------------afeksltertKLVAITH 174 Agrobacterium tume...
2E7J_A    104 yssy--------------vaaeraglNIALVpktdypdyaitpen---------------faqtieetkkrgeVVLALIT 154 Archaeoglobus fulg...
1N8P_A    104 ggthry----------ftkvanahgvETSFTndllndl----------------------------pqlikenTKLVWIE 145 Saccharomyces cere...
1AJS_A    141 enhng-------------vfttagfkDIRSYrywdtekrgldl------------------qgflsdlenapeFSIFVLH 189 pig

Feature 1                                                                                #    
2EO5_A    220 piqgeggyvippknffaelqklakkygILLVDDEVQmglgrtgklf------------aienfntvpDVITLA--KAlgg 285 Sulfolobus tokodai...
1H0C_A    155 hgesstgvlqpl----dgfgelchrykCLLLVDSVAslggtpl-----------------ymdrqgiDILYSGsqKAlna 213 human
1WYU_B    211 npntlglferri----leisrlckeagVQLYYDGANlnaimgwa----------------rpgdmgfDVVHLNlhKTftv 270 Thermus thermophilus
1BJ4_A    192 tscysrnleya------rlrkiadengAYLMADMAHisglvaagv--------------vpspfehcHVVTTTthKTlrg 251 human
1JS6_A    243 lgttsccsfdnl----levgpicheedIWLHVDAAYagsaficpef-----------rhllngvefaDSFNFNphKWllv 307 pig
2TPL_B    182 vtvnlaggqpvsmanmravreltaahgIKVFYDATRcvenayfikeqeqgfenksiaeivhemfsyaDGCTMSgkXDclv 261 Citrobacter freundii
NP_354805 175 msnalgtilpv-----keicriahergIPVLVDGSQgavhmpv-----------------dvrdidcDWYVMTghKLygp 232 Agrobacterium tume...
2E7J_A    155 ypdgnygnlpdv----kkiakvcseydVPLLVNGAYaigrmpv-----------------slkeigaDFIVGSghKSmaa 213 Archaeoglobus fulg...
1N8P_A    146 tptnptlkvtdiqkvadlikkhaagqdVILVVDNTFlspyisn------------------plnfgaDIVVHSatKYing 207 Saccharomyces cere...
1AJS_A    190 acahnptgtdptpeqwkqiasvmkrrfLFPFFDSAYqgfasgnlekda-------wairyfvsegfeLFCAQSfsKNfgl 262 pig

Feature 1                    
2EO5_A    286 g----impIGATIFR 296 Sulfolobus tokodaii str. 7
1H0C_A    214 -----ppgTSLISFS 223 human
1WYU_B    271 phggggpgSGPVGVK 285 Thermus thermophilus
1BJ4_A    252 ------crAGMIFYR 260 human
1JS6_A    308 -----nfdCSAMWVK 317 pig
2TPL_B    262 ------niGGFLCMN 270 Citrobacter freundii
NP_354805 233 ------sgIGVLYGK 241 Agrobacterium tumefaciens str. C58
2E7J_A    214 -----sgpIGVMGMK 223 Archaeoglobus fulgidus
1N8P_A    208 h---sdvvLGVLATN 219 Saccharomyces cerevisiae
1AJS_A    263 y----nerVGNLTVV 273 pig

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