Conserved Protein Domain Family
Peptidase_S66_mccF_like

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cd07062: Peptidase_S66_mccF_like 
Microcin C7 self-immunity protein determines resistance to exogenous microcin C7.
Microcin C7 self-immunity protein (mccF): MccF, a homolog of the LD-carboxypeptidase family, mediates resistance against exogenously added microcin C7 (MccC7), a ribosomally-encoded peptide antibiotic that contains a phosphoramidate linkage to adenosine monophosphate at its C-terminus. The plasmid-encoded mccF gene is transcribed in the opposite direction to the other five genes (mccA-E) and is required for the full expression of immunity but not for production. The catalytic triad residues (Ser, His, Glu) of LD-carboxypeptidase are also conserved in MccF, strongly suggesting that MccF shares the hydrolytic activity with LD-carboxypeptidases. Substrates of MccF have not been deduced, but could likely be microcin C7 precursors. The possible role of MccF is to defend producer cells against exogenous microcin from re-entering after having been exported. It is suggested that MccF is involved in microcin degradation or sequestration in the periplasm.
Statistics
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PSSM-Id: 132883
View PSSM: cd07062
Aligned: 74 rows
Threshold Bit Score: 230.535
Threshold Setting Gi: 104774095
Created: 13-Jan-2009
Updated: 17-Jan-2013
Structure
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Aligned Rows:
 
catalytic triadputative dimer
Feature 1:catalytic triad [active site]
Evidence:
  • Comment:The role of the catalytic triad residues is based on mutational studies for Pseudomonas aeruginosa LD-carboxypeptidase

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
ZP_01859199   11 DTIGIITPSTPAPvlf-seRYERGLAQLRKmGFNVIEGKCsklsqg--yrsasiRDRAEEINDFIRNpeiKAIISTIGGT 87  Bacillus sp. SG-1
YP_076246     15 DLVGVVAPSGPVDar--reAVEAGIRRLEAyGFRVRTGEAlwareg--vragarETQLADLHAMWADpevKAIFCATGGV 90  Symbiobacterium...
ZP_01078558   11 SVIAITAFSSGISegk-kaRYQHVITGLKNqGYVIIEGDClignsq--hvsapaRQRATELMSFLLDdriDAIAAPWGGE 87  Marinomonas sp....
ZP_01899332   11 STIAITAFSSGIEkkh-daRFRVVRENLESrGFNVVVGKClygqrk--hvsapaQKRADELMSFLMDdeiDAIYPPWGGQ 87  Moritella sp. PE36
ZP_02027566   11 DTIGVTATSSGIVnelkqkRIKNAIKNFENrGYNVKVTDNvytsdwr-gcsstgKERGEQFNELVKNkdvSCIFSVAGGD 89  Eubacterium ven...
YP_001557759  11 ATIGVTACSDGKPdvldavRLDHAKVQLDKfGYKVLETKNtrtsfk--nrsssaMERKEQLEELLENdevKAIIMVSGGD 88  Clostridium phy...
ZP_02205675   11 DTIGFVAPSFGCDtepyktAFGRAQENFRNmGFGIQIGPNcyvgegi-gisnkpELCGQEINDYYTSeknDALISCGGGE 89  Coprococcus eut...
YP_001821968  13 DRVGVTSPSSGVPael-reRLAVAVRDVEArGYEVVTGRCmdgagh---vsapaADRAAELTAMLTDpgiRAVVPPWGGE 88  Streptomyces gr...
CAQ64705      14 DLVVVTALSGQLEpge-epLLARGVAILERmGFRVRVSPLvdparprwwaagtpAEQAAELNALLRDpevRAVVAHTGGQ 92  Streptomyces la...
ZP_03487583   12 NTIGVCAMSSGVGhk--ldSFDESMDYIQSfGFHIVESSSvrndse---pscdaLTRVDELNALVKDssiGAVWLAAGGD 86  Eubacterium bif...
Feature 1                                 #                                                      
ZP_01859199   88 NSNSLLLYIDYETLr-kNPKIIMGYSDVTALLLGIYsrtglTTFYGPaivpsfge---ypemlpkGREYFENTVMQTHec 163 Bacillus sp. SG-1
YP_076246     91 TAITLVDGLDYDLIa-qNPKPFIGMSDITLLQAAMLrrtglVSLHASgladgl-------gspdaELEAPYLLRLLTNpd 162 Symbiobacterium...
ZP_01078558   88 LAMEILPLLDFDLLkkaKPKWLFGFSDVSTLTVVISsrlnwASAHSPnlmdlaase-tealtanfLKNMATDTGGRFSqc 166 Marinomonas sp....
ZP_01899332   88 LAIELLPLIDFAKLqtvRPKWILGFSDVSTIAAVFTsklswATGHCSnlmdltsea-ndpltantLMHLSTSIGGSFSqt 166 Moritella sp. PE36
ZP_02027566   90 YLMEMLEYVDFESIk-nNPKWFQGFSDNTTLIYPIVtkcdvAAVYGChisdfgm----kpwqkpvENALGVLEGTVKKqe 164 Eubacterium ven...
YP_001557759  89 YLLEMLPLLDYDKFv-kNPKWLQGYSDPTGLLYTVTvgcdiATVYGCnfsdfgm----gewhhslENNIKLLQGEAVKqd 163 Clostridium phy...
ZP_02205675   90 LMCEILNYIDFDRLraaDPKWFMGYSDNTNLTFLLTtlcdtASIYGPcaaafgq----epwhrsvADAMAVLQGQKLSfa 165 Coprococcus eut...
YP_001821968  89 TAIDLLPLLDWERLrtaEPTWVVGYSDMSTVMTPLTlltgaATVHGNnlmdtpyr--vpeglvswLDVVAAPSGHRFTqi 166 Streptomyces gr...
CAQ64705      93 ATIGYLDLIDLDAIr-aDPKPIIGYSDISLLHMALHaqtglVGFHADiathgfgsdwytlvdearRSELVDLYTRVLTra 171 Streptomyces la...
ZP_03487583   87 FQFETLPYIDFEWIe-kNPKWYLGASDPTNLLFPVTckcdlATVYGFnagsfda----ygvneysNLCLDFLKGKNIElh 161 Eubacterium bif...
Feature 1                                                                                        
ZP_01859199  164 phtlei-----psewteemLDWRTQDrakklvpnsgwnslrKGKTRGKLIGGNLNTMSGIIASeyfpd--------lkga 230 Bacillus sp. SG-1
YP_076246    163 ppgpl-------pdwagevRPVRPGRaesaghdrggasgrqDSGVRGRLVGGCCNCVLHLLGTsywpd--------tega 227 Symbiobacterium...
ZP_01078558  167 aslqhtdk--wpdgtrhpaAVLAPYLktnwcw---lvaprnGSRIEGRLIGGCWDTLIHLFDTpyldlkry-aeqfdegi 240 Marinomonas sp....
ZP_01899332  167 esklyass--wpdivteptSGIMPDTptnwkw---lvkpefGSSIEGRLIGGCWDTLSHLFETeyldlkgl-sqrysegi 240 Moritella sp. PE36
ZP_02027566  165 sfdfyes---drheyvtgfEGYCNDEkvnwvn----grgeeKIEISGRLVGGCLDVLAFLIGTkydgteqfiekykddgi 237 Eubacterium ven...
YP_001557759 164 sfdfymd---gfkkkgtgyEGYDSDKevcwrn----argedEVLIEGRFLGGCLDVLLNLVGTkydkttqfiekykedgi 236 Clostridium phy...
ZP_02205675  166 gydgwe-----keslrdeeHPFLPYNiteprv---ikgypeKFQVSGRMIGGCMDCLVNLIGTkfdhvgeflerykddgf 237 Coprococcus eut...
YP_001821968 167 ppgrhratgyddyaahpgvRTFTLDTpgrwtr----ldgdgDVDVEGRLIGGCVEMLSNLTGTpyldvpafagahapegl 242 Streptomyces gr...
CAQ64705     172 ------------------eAPGALPAngpw-------etwrPGRAQGRLFGGLLNRLVLLQATpfappa-----elfdga 221 Streptomyces la...
ZP_03487583  162 ssslhqd----vdfyndgaPILNTKTey-----------dgSCSVSGRLLGGCFESINDMAGTpydfveefneryksdgv 226 Eubacterium bif...
Feature 1            #                                                                          #
ZP_01859199  231 ILFIEDsfkdmAIQERSLSMLKLaGVLDDi----aGLIVGKHEhfndc---hapftFEDLLMEVmgdrqiPIMTNVDIGH 303 Bacillus sp. SG-1
YP_076246    228 ILFLEGvnlltSAVLRGLAQLRLaGALDGvkailfGHMEGCFRdrps-----plegMALAVTEAlgdldlPVYQTEAFGH 302 Symbiobacterium...
ZP_01078558  241 ILYLENvemapADLTRSILSMKFrGVFTHi----tALLLGRSAypasnn--kedltYHEVLQRHladlgiPVLIDMDIGH 314 Marinomonas sp....
ZP_01899332  241 VLYLENaemspTELARTILNMKFrGVFSCi----sGLLLGRSAaavpks--dqslsYYEVLEKYlmdigiPVMIDLDIGH 314 Moritella sp. PE36
ZP_02027566  238 VWVLESfnmedVVLITHLWQMKEkGYFKNa----tGFVFGRPLmyntw----ieqpYKDAVMSVlgdlnvPIIFDSDIGH 309 Eubacterium ven...
YP_001557759 237 VWYLESfsrdsEELFTGLWNLREaGWFQYa----kGFVFGRPCffeth----tdttYEEAILSVleelnvPVIFDVDFGH 308 Clostridium phy...
ZP_02205675  238 IWFLEScdlnvMSIRRAMWQMDNgGWFRYc----kGFMIGRPLqfgqe---mmgldQYQAVLGIigkynvPVVMDVDLGH 310 Coprococcus eut...
YP_001821968 243 LVYVEAgghdaFTICRNLHGMRLaGFFDAa----nAVLVARTSapds-----gsftQRQAVLDAlgplnvPIVADVECGH 313 Streptomyces gr...
CAQ64705     222 VLFWEElqrpvSRIWNDLHTLRLsGVLDRi----aGMVVGIPTevtphdgggrhvdLRDVVLDVlgqrdiPVLAQVDFGH 297 Streptomyces la...
ZP_03487583  227 IWFFDIfsmnsCDVYRALLKMKYmGYFKYt----kAILVGRVLfknvs----dligYEEAFKRAlp--diDVVFKMDIGH 296 Eubacterium bif...
Feature 1                       
ZP_01859199  304 TFPshvFPIGIEAEL 318 Bacillus sp. SG-1
YP_076246    303 CVPnvtLPIGAVACI 317 Symbiobacterium thermophilum IAM 14863
ZP_01078558  315 VPPnltLINGALAKV 329 Marinomonas sp. MED121
ZP_01899332  315 VPPnltLINGAIVKV 329 Moritella sp. PE36
ZP_02027566  310 KGPqfsVIMGAKAKV 324 Eubacterium ventriosum ATCC 27560
YP_001557759 309 KPPrmaIINGAKGKV 323 Clostridium phytofermentans ISDg
ZP_02205675  311 LSPmmpVVCGSMGTL 325 Coprococcus eutactus ATCC 27759
YP_001821968 314 VQPylpLVNGARGRV 328 Streptomyces griseus subsp. griseus NBRC 13350
CAQ64705     298 TSPnlpLPIGVRADV 312 Streptomyces lasaliensis
ZP_03487583  297 TYPhmyVVNGAMARV 311 Eubacterium biforme DSM 3989

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