3UG9


Conserved Protein Domain Family
7tm_ChRs

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cd15241: 7tm_ChRs 
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channelrhodopsins, member of the seven-transmembrane GPCR superfamily
Channelrhodopsins (ChRs) are light-gated ion channels acting as sensory photoreceptors in unicellular green algae, controlling phototaxis (directional movement toward or away from light). ChRs are large seven-transmembrane proteins with large C-terminal extensions, which have been implicated in localizing the channel to the algal eyespot, a single layer of pigmented granules, overlaying part of the plasma membrane but are not required for ion channel function. ChRs are belongs to the microbial rhodopsin family, also known as type I rhodopsins, comprising the light-driven inward chloride pump halorhodopsin (HR), the outward proton pump bacteriorhodopsin (BR), the light-sensor activating transmembrane transducer protein sensory rhodopsin II (SRII), the light-sensor activating soluble transducer protein Anabaena sensory rhodopsin (ASR), and the other light-driven proton pumps such as blue-light absorbing and green-light absorbing proteorhodopsins, among others. Microbial rhodopsins have been found in various single-celled microorganisms from all three domains of life, including halophile archaea, gamma-proteobacteria, cyanobacteria, fungi, and green algae. While microbial (type 1) and animal (type 2) rhodopsins have no sequence similarity with each other, they share a common architecture consisting of seven-transmembrane alpha-helices (TM) connected by extracellular loops and intracellular loops. Both types of rhodopsins consist of opsin and a covalently attached retinal (the aldehyde of vitamin A), a photoreactive chromophore, via a protonated Schiff base linkage to an amino group of lysine in the middle of the seventh transmembrane helix (TM7). Upon the absorption of light, microbial rhodopsins undergo light-induced photoisomerization of all-trans retinal into the 13-cis isomer, whereas the photoisomerization of 11-cis retinal to all-trans isomer occurs in the animal rhodopsins. While animal visual rhodopsins are activated by light to catalyze GDP/GTP exchange in the alpha subunit of the retinal G protein transducin (Gt), microbial rhodopsins do not activate G proteins, but instead can function as light-dependent ion pumps, cation channels, and sensors.
Statistics
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PSSM-Id: 320369
Aligned: 15 rows
Threshold Bit Score: 385.521
Created: 16-Feb-2014
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:putative ligand binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                            #  #  ## 
3UG9_A     66 LAANILQWITFALSALCLMFYGYQTWKSTCgWEEIYVATIEMIKFIIEYFHEFDEPAVIYssngnktvWLRYAEWLLTCP 145 Chlamydomonas rein...
AGF84748  105 DAAMVWAYIVFAICIIQLVYFMFAAWKATVgWEEVYVNVIELVHIALVIWIEFDKPAMLYlndgqmvpWLRYSAWLLSCP 184 Dunaliella salina
AER58217   97 TFAHVCQWSIFAVCVLSLLWYAYQYWKATCgWEEVYVCCIELVFICFELYHEFDSPCSLYlstsnvvnWLRYSEWLLCCP 176 Chlamydomonas yell...
AER58219   88 LGAIVAMFIVFGSCVGALIFYGVAAWRTTCgWEEVYVCVIELAHVLIAIFHEIESPSTLYlstgnqvlWLRYAEWLLSCP 167 Haematococcus pluv...
AHH02104   63 VAAFAIAWVVFSLSVLQLLYYAYAQWRSTCgWEEVYVGIIELTHICIAIFREFDSPAMLYlstgnfvvWARYASWLLSCP 142 synthetic construct
AER58218   94 AASIAFQWLAFATAVIGLMFYAWDTWKATTgWEEVYVCTIELIKVLIEIFKEFEIPCSLYlptgnwvlWLRYAEWLLTCP 173 Chlamydomonas raud...
AHH02120   50 SLSNACDWFAFAISVIFLVYYAWAAFNSSVgWEEIYVCTVELIKVSIDQFLSSNSPCTLYlstgnrvlWIRYGEWLLTCP 129 synthetic construct
AHH02119   50 LGANVCMWMAFAACLLCLMFYAYSTWRATCgWEEVYVCLVEMVKVMIEVFHENDSPATLYlstgnfimWIRYGEWLLSCP 129 synthetic construct
AHH02148  128 KVLRGFQWVAFAFSLGVLAYYAYNSWKATTgWEEVYVCIVELIKVCLEIWHEPDHPATLYlsagnfilWLRYGEWLLTCP 207 synthetic construct
AHH02106   67 KIAVGLQWFTVIVAIVQLIFYGWHSFKATTgWEEVYVCVIELVKCFIELFHEVDSPATVYqtnggaviWLRYSMWLLTCP 146 synthetic construct
Feature 1      #                           #   #               #  ##  #                       
3UG9_A    146 VILIHLSNLTGLandYNKRTMGLLVSDIGTIVWGTTAALskg-yvRVIFFLMGLCYGIYTFFNAAKVYIEAYhtvPKGRC 224 Chlamydomonas rein...
AGF84748  185 VILIHLSNLTGLkgdYSKRTMGLLVSDIGTIVFGTSAALsppnhvKVILFIIGLLYGLYTFFTAAKVYIEAYhtvPKGHC 264 Dunaliella salina
AER58217  177 VILIHLSNVTGLsddYGRRTMGLLVSDIATIVFGVTAAMlvn-wpKIIFYLIGFTMCCYTFFLAAKVLIESFhqvPKGIC 255 Chlamydomonas yell...
AER58219  168 VILIHLSNLTGMkddYSKRTMGLLISDIGTIVFGTTAAMsppnylKVIFWFCGLSYGVTTFYLAAKVYIEAYhtvPKGIC 247 Haematococcus pluv...
AHH02104  143 VILIHLSNLTGMkgnYSKRTMALLVSDIGTIVWGSTSAMsphnhvKIIFFFLGLVFGLFTFYAAAKVYLEAYhtvPKGKC 222 synthetic construct
AER58218  174 VILIHLSNITGLkddYNKRTMRLLVSDIGCIVWGVTSAMtvg-ylKWIFFAIGLLYGSNTYFHSAKVYIEAYhtvPKGRC 252 Chlamydomonas raud...
AHH02120  130 VILIHLSNVTGLkdnYSKRTMALLVSDIGTIVFGVTSAMctg-ypKVIFFILGCCYGANTFFNAAKVYLEAHhtlPKGSC 208 synthetic construct
AHH02119  130 VILIHLSNITGLqdqYSKRTMQLLVSDLGTITMGVTAALcsn-yvKWIFFIAGLCYGVNTYFHAAKVYIESYhivPKGIC 208 synthetic construct
AHH02148  208 VILIHLSNLTGLkdnYSKRTMMLLISDIGCIVWGVTSALttltwlKWFFFFIGLCYGLSTFFHAGRVYIESYmmlPKGQC 287 synthetic construct
AHH02106  147 VILIHLSNLTGLheeYSKRTMTILVTDIGNIVWGITAAFtkg-plKILFFMIGLFYGVTCFFQIAKVYIESYhtlPKGVC 225 synthetic construct
Feature 1                   #  ##  #                      #  ##           
3UG9_A    225 RQVVTGMAWLFFVSWGMFPILFILGPEGfgvlsvYGSTVGHTIIDLMSKNCWGLLGHYLR 284 Chlamydomonas reinhardtii
AGF84748  265 RNLVRAMAWTYFVSWAMFPILFILGREGfghityYGSSIGHYILEIFSKNLWSLLGHGLR 324 Dunaliella salina
AER58217  256 RHLVKAMAITYFVGWSFFPLIFLFGQSGfkkispYADVIASSFGDLISKNAFGMLGHFLR 315 Chlamydomonas yellowstonensis
AER58219  248 RKIVRFMAWDYFGSWCMFPILFVLGPEGfghisaYGSVIAHQVLDITSKNIWSMAGHFLR 307 Haematococcus pluvialis
AHH02104  223 RNIVRFMAWTYYVTWALFPILFILGPEGfghityYGSSIGHYVLEIFSKNLWSGTGHYLR 282 synthetic construct
AER58218  253 RVIVRLMAYCFYLAWTMFPILFALGPEGmgqmsaYMSTILTTIADVLSKQIWGLLGHHLR 312 Chlamydomonas raudensis
AHH02120  209 RTLIRLMAYTYYASWGMFPILFVLGPESfghmnmYQSNIAHTVIDLMSKNIWGMLGHFLR 268 synthetic construct
AHH02119  209 RICVRVMAWCFFAAWTCYPLLFVFGPEGlgvlsyNASAIGHTIIDIFSKQVWGFVGHYLR 268 synthetic construct
AHH02148  288 RKILRIMAGVYYFSWSAFPILFILGPEGfghisaYASTIGHTINDMLSKQLWTLLGHHLR 347 synthetic construct
AHH02106  226 RKICKIMAYVFFCSWLMFPVMFIAGHEGlglitpYTSGIGHLILDLISKNTWGFLGHHLR 285 synthetic construct

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