4QJ3,4GNK,2FJU,3QR0


Conserved Protein Domain Family
EFh_PI-PLCbeta

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cd16200: EFh_PI-PLCbeta 
Click on image for an interactive view with Cn3D
EF-hand motif found in metazoan phosphoinositide-specific phospholipase C (PI-PLC)-beta isozymes
PI-PLC-beta isozymes represent a class of metazoan PI-PLCs that hydrolyze the membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2) to propagate diverse intracellular responses that underlie the physiological action of many hormones, neurotransmitters, and growth factors (EC 3.1.4.11). They have been implicated in numerous processes relevant to central nervous system (CNS), including chemotaxis, cardiovascular function, neuronal signaling, and opioid sensitivity. Like other PI-PLC isozymes, PI-PLC-beta isozymes contain a core set of domains, including an N-terminal pleckstrin homology (PH) domain, four atypical EF-hand motifs, a PLC catalytic core, and a single C2 domain. Besides, they have a unique C-terminal coiled-coil (CT) domain necessary for homodimerization. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence. There are four PI-PLC-beta isozymes (1-4). PI-PLC-beta1 and PI-PLC-beta3 are expressed in a wide range of tissues and cell types, whereas PI-PLC-beta2 and PI-PLC-beta4 have been found only in hematopoietic and neuronal tissues, respectively. All PI-PLC-beta isozymes are activated by the heterotrimeric G protein alpha subunits of the Gq class through their C2 domain and long C-terminal extension. They are GTPase-activating proteins (GAPs) for these G alpha(q) proteins. PI-PLC-beta2 and PI-PLC-beta3 can also be activated by beta-gamma subunits of the G alpha(i/o) family of heterotrimeric G proteins and the small GTPases such as Rac and Cdc42. This family also includes two invertebrate homologs of PI-PLC-beta, PLC21 from cephalopod retina and No receptor potential A protein (NorpA) from Drosophila melanogaster.
Statistics
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PSSM-Id: 320030
Aligned: 21 rows
Threshold Bit Score: 146.622
Created: 17-Nov-2014
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 30 residues -Click on image for an interactive view with Cn3D
Feature 1:EF-hand motif [structural motif]
Evidence:
  • None

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         ##########   ####################                                               
4QJ3_B        155 RKAYTKLKLQ---VNQDGRIPVKNILKMFSADKkr--vETALESCGLk----fNRSESIRPDEFSLEIFERFlnkl--cl 223  human
XP_009858419  171 EKAYTRLKLE---TNQFGEIPVKKLIKVLGGKIkr--vNHALASVGLvp---gRTDVSIKPDDFHLSGFKKLlqlc--cl 240  vase tunicate
ERG82323      147 QKLFAPIRYAsckELITIDDIVKIIVPCESNISdstriHKALMASELf-----FGKNGIPRTSFTDEHIFDIymai--td 219  pig roundworm
BAA76277      152 YKQYVLSKSM---VNASGKISMKNIFKCLNAVRf---eQTTIEGIQTal---sLPVGEIDPEMFSFEQFYKLcclv--cp 220  Hydra vulgaris
BAA76274      153 KRNYQRILLS---CNGYGQIPVKSICRALLLRVrdktdMELFGIFDAqkkkmdDGTEYIQDTDFTWDKYEQLvnqltkgr 229  Ephydatia flu...
XP_003386377  152 RKHYVRMCLE---SNGHGQLPVKVVRKLMMVPKtskdiLNYFESAKTnvkekdDGTPYIDVSEFTEEIYMNLidtllqnr 228  Amphimedon qu...
KHN82931      146 QKLLAPLRYAnckESITVDDIVKAIVPCEGNTTdsahvHKALIADELf-----FKKKYMLRSELTDERIFEIymai--td 218  dog roundworm
KHN74602      167 QRLFTKISHSsgeDFITVNDIVDALIPSKNKEDrr-evERLLKLIPTf-----KDKKSIPSDAITDDFIFTCyrti--td 238  dog roundworm
AFK83791       74 RKMHKRILLS---SKDDKNISIKEIIKMTAKDEkk-rvQEALKAAGIka--ddRKNDLFKKDKFTFEKFHKFykeml-ny 146  sea walnut
XP_009861182  140 RKQWKRIYLP---INNKGLIPVRRLVKSFTSDRnekvvLQKLKTCGLi----yGKKDEISQSEFSFEFYQKLynkm--cs 210  vase tunicate
Feature 1                                                                                         
4QJ3_B        224 rpDIDKILLEIga-----kGKPYLTLEQLMDFINQKQRDPRLNEVLYPplrpsQARLLIEKYepnqqFLERDQMSMEGFS 298  human
XP_009858419  241 rpELDSVFSKLgt----gaRRMYVSHQKFKDFINNEQRDPKLNDLLYPfhtddQAQDAITNFepnemFATKGQLTLEGLD 316  vase tunicate
ERG82323      220 rrEIYHIFARRtrdrktriLPGRMELKKVKRFLNHVQRDPRQNEVIFPeecnqSVKKIIDKF-----NGNRKFLTEKAFA 294  pig roundworm
BAA76277      221 rpDIDELFISL--------FGTRLTAEKLMKFLNEDQRDPRLNEQMHPhctisNAVDLIMDYesneaCRTKELLTIQGFI 292  Hydra vulgaris
BAA76274      230 tsDMECVIKDLgk----skKHQQLTLQEFHNFLTKCQRDPRLNTILYPpptkqQAKELAEKYe---gKAVEDRLTPRALL 302  Ephydatia flu...
XP_003386377  229 gpDLDVLKKECki----kaRKQYIDAKEFATVLNTQQRDPRLNDILHPrltneQAMALMDKY-----GGEKDRLTMKDLL 299  Amphimedon qu...
KHN82931      219 rrDIRHVFARRtrdrrtrtLPGKMELKKVRRFINHVQRDPRQNELIFPeesleSIKKTVYKF-----AGNRKFLTEKGFA 293  dog roundworm
KHN74602      239 rtDVQSVFNERf------qNERRVTSDLFAKYLKKEHYDPRLNELLYPspnedSAQMLIDEF-----DKTDCCLNVDAFH 307  dog roundworm
AFK83791      147 lpGVSKVFDEIkg----dnRRNYLTIDQMVDFLNKFQRDPRLNEILFPhhnkeGTKKLIQSYeidksKQKIGQLTLDGFT 222  sea walnut
XP_009861182  211 rgDVENIFHEIs-------HNDIIDIDALVEWMNDNQRDPRHNEILVPafdrkQAANIIQKFepsqrNIKNNCLSLEGFT 283  vase tunicate
Feature 1                
4QJ3_B        299 RYLGGEE 305  human
XP_009858419  317 AYLQSDD 323  vase tunicate
ERG82323      295 KYLLSDE 301  pig roundworm
BAA76277      293 QLLLSKE 299  Hydra vulgaris
BAA76274      303 NFLMSED 309  Ephydatia fluviatilis
XP_003386377  300 QYLMSDD 306  Amphimedon queenslandica
KHN82931      294 KYLLSNE 300  dog roundworm
KHN74602      308 RFLMSSY 314  dog roundworm
AFK83791      223 NFLMSPD 229  sea walnut
XP_009861182  284 LYLASDE 290  vase tunicate

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