4TXA


Conserved Protein Domain Family
mRING-HC-C3HC3D_Roquin1

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cd16781: mRING-HC-C3HC3D_Roquin1 
Click on image for an interactive view with Cn3D
Modified RING finger, HC subclass (C3HC3D-type), found in Roquin-1
Roquin-1, also known as RING finger and C3H zinc finger protein 1 (RC3H1), or RING finger protein 198 (RNF198), is a ubiquitously expressed RNA-binding protein essential for degradation of inflammation-related mRNAs and maintenance of immune homeostasis. It is localized in cytoplasmic granules and binds to the 3' untranslated region (3'UTR) of inducible costimulator (Icos) mRNA to post-transcriptionally repress its expression. Roquin-1 interacts with the 3'UTR of tumor necrosis factor receptor superfamily member 4 (TNFRSF4) and tumor-necrosis factor-alpha (TNFalpha), and post-transcriptionally regulates A20 mRNA and modulates the activity of the IKK/NF-kappaB pathway. Moreover, Roquin-1 shares functions with its paralog Roquin-2 in the repression of mRNAs controlling T follicular helper cells and systemic inflammation. Roquin-1 contains an N-terminal modified C3HC3D-type RING-HC finger with a potential E3 ubiquitin-ligase function, a highly conserved ROQ domain required for RNA binding and localization to stress granules, and a CCCH-type zinc finger that is involved in RNA recognition, typically contacting AU-rich elements. In addition, both N- and C-terminal to the ROQ domain are combined to form a HEPN (higher eukaryotes and prokaryotes nucleotide-binding) domain that is highly likely to function as an RNA-binding domain.
Statistics
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PSSM-Id: 438436
Aligned: 6 rows
Threshold Bit Score: 117.418
Created: 19-Mar-2015
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
 
Zn binding site
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1: Zn binding site [ion binding site], 8 residue positions
Conserved feature residue pattern:C C C H C C C [DE]Click to see conserved feature residue pattern help
Evidence:
  • Structure:4TXA; Mus musculus Roquin binds two Zn2+ ions, contacts at 4A.
    View structure with Cn3D
  • Comment:modified RING-HC finger (C3HC3D-type)
  • Comment:consensus of the typical C3HC4-type RING-HC finger: C-X2-C-X(9-39)-C-X(1-3)-H-X(2-3)-C-X2-C-X(4-48)-C-X2-C, X is any amino acid and the number of X residues varies in different fingers.
  • Comment:A RING finger typically binds two zinc atoms, with its Cys and/or His side chains in a unique "cross-brace" arrangement.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 #  #               # #  #  #        #  # 
4TXA_A          6 PQWTDFLSCPICTQTFDETiRKPISLGCGHTVCKMCLNKLHRKACPFDQ 54   house mouse
Q5TC82          6 PQWTDFLSCPICTQTFDETiRKPISLGCGHTVCKMCLNKLHRKACPFDQ 54   human
NP_001108155    6 PQWTEFLLCPICTQTFEETvRRPISLGCGHTVCKMCLNKLHRKACPFDQ 54   zebrafish
Q6NUC6          6 PQWTDFLSCPICTQTFDETiRKPISLGCGHTVCKMCLNKLHRKACPFDQ 54   African clawed frog
XP_007901124    6 PQWTDFLSCPICCRTFDENvRKPISLGCGHTLCSICLNNLHRKACPFDH 54   elephant shark
XP_014342758    6 PQWTDFLSCPICTQTFDETiRKPISLGCGHTVCKMCLNKLHRKACPFDQ 54   coelacanth

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