Conserved Protein Domain Family
RAM

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pfam15320: RAM 
mRNA cap methylation, RNMT-activating mini protein
This family of proteins is found in eukaryotes. Proteins in this family are typically between 102 and 154 amino acids in length. There is a single completely conserved residue D that may be functionally important. RAM is a family of eukaryotic proteins that are an obligate component of the mammalian cap methyltransferase, RNMT (RNA guanine-7 methyltransferase). RAM consists of an N-terminal RNMT-activating domain and a C-terminal RNA-binding domain. Either RAM or RNMT independently have rather weak binding affinity for RNA, but together their RNA affinity is significantly increased. RAM is necessary for efficient cap methylation, maintaining mRNA expression levels, for mRNA translation and for cell viability.
Statistics
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PSSM-Id: 317693
View PSSM: pfam15320
Aligned: 28 rows
Threshold Bit Score: 43.1022
Threshold Setting Gi: 74959882
Created: 6-Apr-2017
Updated: 23-May-2017
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 123893237   10 LYEKMFEQRFTANDKEYQEYLKREQDQPPIVEDW-------------K-------------MGNqr--ntD---RYR--D 56
gi 74959882     6 SLDELFKDRYSQNDERYKKMSETGFEPVIVVHPW-------------EprrnnnyrnrgnfGGG----------RGGgyQ 62
gi 801382643   16 ECEEEFKDRYTEKDDDFMKIKTQEPKKPPIVDPW-------------Y-------------NKPrr--hdWs--RQN--Q 63
gi 642922973   15 ECALEFSDRFTDADFEYKKIFDLGIPKPPIMSPW-------------Ysk-----------------------aRFN--R 56
gi 1036739438  21 ECELEFSGRFTDDDPEFMAHCSKPFPDPPIVENWiggggggggggggGgr----------dGGHpy--rnHn--RFN--G 84
gi 268568576    6 SLDELFKDRYTEKDERYKKMAETGFEKVIVVHPW-------------Dprr--------------------nnfRGRggS 52
gi 541042555   76 ELDAMFANRFTVDDDLYAEVSN-GFPKPIVIYPW-------------AsrpkrnydyvrrrGG-----------AQEdgE 130
gi 645011389   14 ECEKEFKDRFTEKDEDFMKVKLGESLKPPIVDPW-------------Nearq-----rnrtGQRniqsprItssRQN--R 73
gi 645002378   14 ECQEEFQDRFTEKDQDFMKVKSGEPKKPPIVDPW-------------Yntqq---------------ssrNnysRQN--- 62
gi 357624323   16 KCEAEFKDRFTERDEEYMKVVNRDPPTPPILENW-------------LssnssgrrhdrryNN-----------RHNtyD 71
                          90       100       110
                  ....*....|....*....|....*....|..
gi 123893237   57 NRHH--RGWDGRqnwsSN------SYNqsyGR 80
gi 74959882    63 HRDNsnRGGWVPrgggQRg-----GYRggwQD 89
gi 801382643   64 NQNHdrRNHHWDrrnnERn-----EKLerhGG 90
gi 642922973   57 DRGEnrYNERRDyrsdNHr-----DRSsyyKD 83
gi 1036739438  85 HQQRg---gDRGwqrrGGg-----GYHnhhND 108
gi 268568576   53 GYNNqnRGGWVPrgegGGgrgqygGYRggwQD 84
gi 541042555  131 STGKrwRGREDSrrsgNSw-----------RG 151
gi 645011389   74 KRTKrkRDRTSDrgrgDRe-----RDRsrsRR 100
gi 645002378   63 -HGRdrRNRDRDrgrgDRd-----RYGdrgRG 88
gi 357624323   72 RYGNrdRRARGDynrhGRsq---------yDN 94
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