2L2I


Conserved Protein Domain Family
EKLF_TAD2

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pfam16833: EKLF_TAD2 
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Erythroid krueppel-like transcription factor, transactivation 2
This family is the second part of the minimal transactivation domain of erythroid-specific transcription factor EKFL in craniates. EKLF plays an important role in red blood cell development; it is post-translationally modified by ubiquitin on several lysine residues, and its turnover in the cell is regulated by ubiquitin-mediated degradation. In the first 90 residues at the N-terminus EKLF carries a minimal transactivation or TAD domain that is highly acidic. This minimal TAD of EKLF can be further subdivided into two independent domains EKLF_TAD1 (residues 1-40), pfam16832, and EKLF_TAD2 (residues 51-90) that are both capable of independently activating transcription. Both TAD1 and TAD2 are highly acidic and carry a PEST (sequence rich in proline, glutamic acid, serine, and threonine) region. Deletion of either PEST domain significantly slows down degradation of EKLF by ubiquitin. The minimal TAD has an overlapping activation/degradation function that is critical for the role of EKLF in red blood cell development.
Statistics
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PSSM-Id: 318932
View PSSM: pfam16833
Aligned: 12 rows
Threshold Bit Score: 35.9021
Threshold Setting Gi: 821466716
Created: 31-Jul-2016
Updated: 4-Aug-2016
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
                          10        20        30
                  ....*....|....*....|....*....|
2L2I_B         11 PgEEEDDERGADAT---WDLDLLLTNFSGP 37
gi 821466716   65 P-EQVEGDRGAEANdpcWDLDFFLTNFPGP 93
gi 562865751   61 PgEDEDDDKDAAAA---WDLDLFLNNFPSP 87
gi 984106500   61 PrENEDNEKDAAAD---WDLSLLLTNFPGP 87
gi 395850802   59 PrEDED-HRDADAT---WDLDLLLTNFPGP 84
gi 345787752   61 PgEDEDDERDSATA---WDLDLLLTNFPCP 87
gi 759185238   61 HgEEEDEERDTATA---WDLDLFLTNLPCS 87
gi 558199155   61 PgEREDDERDPATA---WDLDLFFTNLSGP 87
gi 1032945885  79 PgEDEDDERDVAYA---WDPDPYLTNFPGP 105
gi 344283261   82 PeEDEDEERDETVA---WDLDLLLTNFPGP 108
gi 513022412   61 PgEDEDGDRDADAA---WDLDLFLSSFPPP 87
gi 1040142184  68 PaEDEDDDQTCTAA---WDLDLSLSNFSSP 94
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