Conserved Protein Domain Family
CNDH2_C

?
pfam16858: CNDH2_C 
Condensin II complex subunit CAP-H2 or CNDH2, C-term
CNDH2_C is the C-terminal domain of the H2 subunit of the condensin II complex, found in eukaryotes but not fungi. Eukaryotes carry at least two condensin complexes, I and II, each made up of five subunits. The functions of the two complexes are collaborative but non-overlapping. CI appears to be functional in G2 phase in the cytoplasm beginning the process of chromosomal lateral compaction while the CII are concentrated in the nucleus, possibly to counteract the activity of cohesion at this stage. In prophase, CII contributes to axial shortening of chromatids while CI continues to bring about lateral chromatid compaction, during which time the sister chromatids are joined centrally by cohesins. There appears to be just one condensin complex in fungi. CI and CII each contain SMC2 and SMC4 (structural maintenance of chromosomes) subunits, then CI has non-SMC CAP-D2 (CND1), CAP-G (CND3), and CAP-H (CND2). CII has, in addition to the two SMCs, CAP-D3, CAPG2 and CAP-H2. All four of the CAP-D and CAP-G subunits have degenerate HEAT repeats, whereas the CAP-H are kleisins or SMC-interacting proteins (ie they bind directly to the SMC subunits in the complex). The SMC molecules are each long with a small hinge-like knob at the free end of a longish strand, articulating with each other at the hinge. Each strand ends in a knob-like head that binds to one or other end of the CAP-H subunit. The HEAT-repeat containing D and G subunits bind side-by-side between the ends of the H subunit. Activity of the various parts of the complex seem to be triggered by extensive phosphorylations, eg, entry of the complex, in Sch.pombe, into the nucleus during mitosis is promoted by Cdk1 phosphorylation of SMC4/Cut3; and it has been shown that Cdk1 phosphorylates CAP-D3 at Thr1415 in He-La cells thus promoting early stage chromosomal condensation by CII.
Statistics
?
PSSM-Id: 318951
View PSSM: pfam16858
Aligned: 24 rows
Threshold Bit Score: 222.58
Threshold Setting Gi: 312089064
Created: 29-Jul-2016
Updated: 4-Aug-2016
Structure
?
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 82077898   291 PWKLHDPY----ATFgEDK--PLKAGKC-YK---------------VP------AGL-DE-------SGKRKRKGP---- 330
gi 219130131  318 PWKLLDPHe---PDSvKSR--PLCIGKT-IR---------------LPagvdepPSAcVTgartrhvAPQRRLRGVidka 376
gi 699252999  228 sQPMLDPHtpcpESLwQPK--PFKKGNC-SR---------------LPsgv--------------stGKKRKKAGC---- 271
gi 312089064  303 dgadedepvmdlfrlqKCKhaPYRACKPsF----------------VPeqv--------------ekRRLKKREKL---- 348
gi 170580982  329 dgadedepvmdlfrlqKCKhgPYRACKPtF----------------VPeqv--------------ekRRLKKREKL---- 374
gi 296083331  902 pWKPLNPHe---AGNlKVK--PFKKVKA-FRrhgvcstkhnilttqFP---------------------LAKMHGP---- 950
gi 223544047  298 PWKPLNPHe---PGTlKVK--PFRKVKP-YRrnvvksarqtsittmFP---------------------LARMRGT---- 346
gi 769815671  420 PWKPLNPHe---PGTlKVK--PFKKGNF-VRarrvshteeysislqFP---------------------VAKLHGT---- 468
gi 301116279  286 PWTPLDAYd---ASSsVAR--PFKKGRSyPR---------------VP-------------------AYKKKKRGS---- 322
gi 195997841  277 aWQVLDPHd---KDAiPER--AFKASRN-WR---------------IP-----------KniaidtqAIRKRKRGQisit 324
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 82077898   331 -----SKLQDFGTWFNKAF------------ETADRKLKNGp------TFPD---------------------------- 359
gi 219130131  377 vgypsLATQTFRSLRKRNHdd---------tREEDAAPERPvlplgglAFGDe--------------------------- 420
gi 699252999  272 -------HDNDASMLGEFMedmmkkrqrfvsKTEEIEKKRR--------------------------------------- 305
gi 312089064  349 -------GLKTFSTLNYFLqr---------hVYHSLPKKTGh------ILDDd--------------------------- 379
gi 170580982  375 ---------KTFSTLNEFLqr---------hVYHSLPKGITh------TLDDd--------------------------- 403
gi 296083331  951 -----ISp----------------------------------------ELTEi--------------------------- 958
gi 223544047  347 -----ISp----------------------------------------ELTEi--------------------------- 354
gi 769815671  469 -----ISp----------------------------------------EFAEa--------------------------- 476
gi 301116279  323 ------------VALSNKEga---------vGMEDEAEQDGls---dpAFQDrffmnangpqwaswvwtetsrenplekt 378
gi 195997841  325 snqpgISIIEFCSRAACTRqn---------qKLLKHAMN---------VICEd--------------------------- 359
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 82077898   360 --------------LNYIFVSKMDQRLKVQrqmlrkrgvfvsDEELKKTYLEPEnveDQVEVIRhpdadgddysdeehdn 425
gi 219130131  421 --------------FAYIAKANAKRKAAER------------REKRKLALLRQK---DEGHVDRyddddeggfafgvddd 471
gi 699252999  306 ----------------MLCWRGLREKQIQNkrk------------hskEVSLVDevfATSFHDDhndddgchgnndvtdd 357
gi 312089064  380 --------------FSIIFGKLIRNLERQRmni-------irERALQNVGLYQQsgdIQSDESEidedeyeaannghsdd 438
gi 170580982  404 --------------FSIIFGKLIRNLERHRmna-------ikERALQHSGLQQQsgdIQSDEDElevdeyeainsgysdd 462
gi 296083331  959 --------------WEK-HRACERQQEPQTpp---------lYEKLRNSLIHGDsqtFSAYSNVednkedggydsgvndf 1014
gi 223544047  355 --------------WEARFSRSEKTESADSpp---------lYEKLRQSLSNGGynnSDTFGNAenddegnehdsgvpdf 411
gi 769815671  477 --------------LEALTNALKNTPASHSpp---------pFEKLRQTLVSASngnCGIHGDPvnenedfkfengfddf 533
gi 301116279  379 ckrnfcktpllvktCDMLWQREAKWRSMMRrce-------arEKQSAQAMLLREqamEEEEVDGqdqmqaltlstata-- 449
gi 195997841  360 --------------LEYISYEFAENQHILSre---------iNKLFKFMTSNGQdsdDHDKHDDqennidggydddysdq 416
                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 82077898   426 laddl------------------------epaehlddqeqifqdlhmsrmSYEDLVKKSVDLFLVNSQKYAQETALSRRV 481
gi 219130131  472 dsdhddhgdfq-------------snggvegntgmssmddlyrshelesqTFEELCRAHLKAFANGAEKYASETQLSKRV 538
gi 699252999  358 vindalgidg--------------lmldveenrggdpeierlqsfvgtleNYEDLVRHHVEKFYASAKSYRNESALSQRV 423
gi 312089064  439 eiedapdvtgdgnltfanmpnddledrvhissmymnfdspdlhlkppeqmTYQDLLKVHLQKYWSSAEETT--SKLCKRV 516
gi 170580982  463 eiegasgvtgdgnltfadipnddledrvhvssmymnfddpdlllkpaeqmTYQDLLKVHLQKYWSSAEETT--SKLCKRV 540
gi 296083331 1015 gdemphdmymdddlpl---hqeqvrlpvlmcqeqnimhhddgaacfdsidNLEDLCRSHLDALLANIAETEKQTELAARV 1091
gi 223544047  412 gqpdddipenmymd-------edlhhekvgssthfdineafgnedpssqaSLEDLCRSHLDALLANIAETEKQTELATRV 484
gi 769815671  534 edadidlpndny----------keneaplqpeqesvggdafvdkdlnaraSLEDLCRAHLDALLASIAESEKQTELAARV 603
gi 301116279  450 -------------------------------------rldmavdnsggplTYEEICRQHLATFMSGTEKYVRETDLSKKV 492
gi 195997841  417 ddfgiennynpd----------qeivnnehnmsidpiaieeqnlenqipqTYEELVKEHLNVYAKVTRQYIEDSALRSRV 486
                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 82077898   482 KDWEDSINPHLAAQEISPAFDIHEYGDRIVHALS-------NV----------------------GVKKSFAFVVR---- 528
gi 219130131  539 NIWQEKLAPVLEAEAQRPVFDIHEYGRNVIDSLEavirrkkTS----------------------GEKTSFARVVDfasv 596
gi 699252999  424 VEWEKSIAPSLSEEDKHPEFDIHTEGDEMVKQFNi-----------------------------vGQTKYFCDVIK---- 470
gi 312089064  517 QQWEEKVQPLLEEEETRREFNIHQYGTELLEQYEg-----------------------------iGQKKTFAELMEn--- 564
gi 170580982  541 QQWEEKIQLLLEKEETRREFNIHQYGTELLEQYEd-----------------------------iGQTKTFTELMEh--- 588
gi 296083331 1092 STWKQKIEHDLEEQDLRPPFDIHDYGDRILEKLShka--------------------------enGNVMSFSEVVS---- 1141
gi 223544047  485 SSWKQKIEHNLEEQDSRPSFDIHAYGERILDKLSlea--------------------------ngENVMSFADVVK---- 534
gi 769815671  604 STWKQKIEHNLEEQDSHPPFDIHAYGERLIEKLSldp--------------------------krNSGLPFTDIVG---- 653
gi 301116279  493 SDWQDKLSPLLKQQDTRPPFDIHLYGREIIGRLEeeqknssLArhskttqrdknkrirlatddeeSETVPFETLVD---- 568
gi 195997841  487 NEWQDRIKPALENEEKHPPFDIHTYGQSVLDSLKek----------------------------nDDAVSFEDVVSqkvl 538
                         410       420       430       440       450       460
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 82077898   529 --G--------------KENTEACRYMLAALQLANDYTVE-VDKVDGl-----dCSVDTMELTLLTT 573
gi 219130131  597 arN--------------RPQYEVCRLFLASLSLSNSGNILpVQSDDD-------NSGNHFKLQLLNT 642
gi 699252999  471 --R--------------EPVWSICRRFSATLQLANDLNFL-LQSEGDpi---mmSSVNTLSLKLLTQ 517
gi 312089064  565 ----------------vSAQYDISRYCLALLMLANTENIE-VDFNYEte---ctNDGRDLALKLLKK 611
gi 170580982  589 ----------------vSTRYDISRYYLASLMLANTGNIE-MDFGCEse---ymKKGKGLALKLLKN 635
gi 296083331 1142 --G--------------QEKHDVARSFSALLQLVNNEDVN-LDRGGTdgesvcyTDANPFYIKLLNR 1191
gi 223544047  535 --G--------------QEKHDVARTFSALLQLVNNGDVN-LERSGVggmsvcyTAMNPFHIQLLNH 584
gi 769815671  654 --G--------------QEKHEVARSFSALLQLVNNGNVV-LDKVSSngaafcfSAANPFYVRLLSS 703
gi 301116279  569 --G--------------KSQFEVCRLFLASLQLANSGNVAlFHARTAa-----eHGQVPFKMELLST 614
gi 195997841  539 trNtyvalyeyysfyfrQEPYEICRLFAATLQLANNGNIE-ISHGGKne----kAPVNTMTVKMLST 600
| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap