NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|237902000|gb|EEP76401|]
View 

conserved hypothetical protein [Uncinocarpus reesii 1704]

Protein Classification

LSB5 family protein( domain architecture ID 13015572)

LSB5 family protein similar to Saccharomyces cerevisiae LAS seventeen-binding protein 5, an essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with YSC84

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
GAT_LSB5 cd14232
canonical GAT domain found in yeast LAS seventeen-binding protein 5 (Lsb5p) and similar ...
 283-360 8.26e-40

canonical GAT domain found in yeast LAS seventeen-binding protein 5 (Lsb5p) and similar proteins; Lsb5p, also called LAS17-binding protein 5, is a Golgi-localized gamma ear-containing Arf-binding protein (GGA)-like protein located to the plasma membrane in an actin-independent manner. It plays important roles in membrane-trafficking events through association with the actin regulators, the yeast Wiskott-Aldrich syndrome protein (WASP) homologue Las17p and the cortical protein Sla1p, the yeast Arf3p (orthologous with mammalian Arf6), and ubiquitin. Lsb5p contains an N-terminal VHS (Vps27p/Hrs/STAM)-domain and a GAT (GGA and TOM1) domain. In contrast to GGA proteins, Lsb5p harbors a C-terminal NPF (Asn-Pro-Phe) motif, but does not have either a GAE (gamma-adaptin ear homology) domain or a clathrin-binding motif. The canonical GAT domain is a monomeric three-helix bundle that binds ubiquitin.


:

Pssm-ID: 410580  Cd Length: 78  Bit Score: 137.69  E-value: 8.26e-40
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 237902000 283 IASASVASTNLTNALKLVNREAQRVSENSDVMEKFERCKSLRRQILRYIQVVESDDFLGSLIHANEELVNALMAFEVL 360
Cdd:cd14232    1 IADASQAATNLVNALQLVNRETERVSDDAEVQERFEKCKQLRRKILRYIQLVEDEEYLGSLLHANEELVAALEAYDEL 78
VHS_Lsb5 cd16980
VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein ...
 95-199 3.24e-32

VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein 5 (LAS17-binding protein 5, Lsb5, or Lsb5p) localizes to the plasma membrane and plays a role in endocytosis in yeast. It interacts with actin regulators Sla1p and Las17p, ubiquitin, and Arf3p, coupling actin dynamics to membrane trafficking processes. Lsb5p contains an N-terminal VHS domain and a GAT (GGA and TOM) domain. The VHS domain has a superhelical structure similar to the structure of ARM (Armadillo) repeats. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting.


:

Pssm-ID: 340777  Cd Length: 132  Bit Score: 119.24  E-value: 3.24e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000  95 VTVQIDRLTSEQYDVDDSSGIVDLIEVIRLQSSGPQEASRALRKKL-------------------------FLKTFA-DE 148
Cdd:cd16980    1 ITDTIERLTSSEYEEEDWSLVLELCELINLIESGAKEAARALRKKLkygnphqqlraltlldllvenggkkFQAQFAsDK 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 237902000 149 PLLERLRVAATDSVSDPDVREKCKQLFPQWAVTYKSTPGMERVALLYKQLP 199
Cdd:cd16980   81 KLEDLLRLIASDPSTDPKVRKKLLELLGGWAYEFKGDPGLSGIASLYKKVK 131
 
Name Accession Description Interval E-value
GAT_LSB5 cd14232
canonical GAT domain found in yeast LAS seventeen-binding protein 5 (Lsb5p) and similar ...
 283-360 8.26e-40

canonical GAT domain found in yeast LAS seventeen-binding protein 5 (Lsb5p) and similar proteins; Lsb5p, also called LAS17-binding protein 5, is a Golgi-localized gamma ear-containing Arf-binding protein (GGA)-like protein located to the plasma membrane in an actin-independent manner. It plays important roles in membrane-trafficking events through association with the actin regulators, the yeast Wiskott-Aldrich syndrome protein (WASP) homologue Las17p and the cortical protein Sla1p, the yeast Arf3p (orthologous with mammalian Arf6), and ubiquitin. Lsb5p contains an N-terminal VHS (Vps27p/Hrs/STAM)-domain and a GAT (GGA and TOM1) domain. In contrast to GGA proteins, Lsb5p harbors a C-terminal NPF (Asn-Pro-Phe) motif, but does not have either a GAE (gamma-adaptin ear homology) domain or a clathrin-binding motif. The canonical GAT domain is a monomeric three-helix bundle that binds ubiquitin.


Pssm-ID: 410580  Cd Length: 78  Bit Score: 137.69  E-value: 8.26e-40
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 237902000 283 IASASVASTNLTNALKLVNREAQRVSENSDVMEKFERCKSLRRQILRYIQVVESDDFLGSLIHANEELVNALMAFEVL 360
Cdd:cd14232    1 IADASQAATNLVNALQLVNRETERVSDDAEVQERFEKCKQLRRKILRYIQLVEDEEYLGSLLHANEELVAALEAYDEL 78
VHS_Lsb5 cd16980
VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein ...
 95-199 3.24e-32

VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein 5 (LAS17-binding protein 5, Lsb5, or Lsb5p) localizes to the plasma membrane and plays a role in endocytosis in yeast. It interacts with actin regulators Sla1p and Las17p, ubiquitin, and Arf3p, coupling actin dynamics to membrane trafficking processes. Lsb5p contains an N-terminal VHS domain and a GAT (GGA and TOM) domain. The VHS domain has a superhelical structure similar to the structure of ARM (Armadillo) repeats. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting.


Pssm-ID: 340777  Cd Length: 132  Bit Score: 119.24  E-value: 3.24e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000  95 VTVQIDRLTSEQYDVDDSSGIVDLIEVIRLQSSGPQEASRALRKKL-------------------------FLKTFA-DE 148
Cdd:cd16980    1 ITDTIERLTSSEYEEEDWSLVLELCELINLIESGAKEAARALRKKLkygnphqqlraltlldllvenggkkFQAQFAsDK 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 237902000 149 PLLERLRVAATDSVSDPDVREKCKQLFPQWAVTYKSTPGMERVALLYKQLP 199
Cdd:cd16980   81 KLEDLLRLIASDPSTDPKVRKKLLELLGGWAYEFKGDPGLSGIASLYKKVK 131
VHS pfam00790
VHS domain; Domain present in VPS-27, Hrs and STAM.
 99-201 2.29e-11

VHS domain; Domain present in VPS-27, Hrs and STAM.


Pssm-ID: 395638  Cd Length: 136  Bit Score: 61.16  E-value: 2.29e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000   99 IDRLTSEQYDVDDSSGIVDLIEVIRLQSSGPQEASRALRKKL-------------------------FLKTFADEPLLER 153
Cdd:pfam00790   6 IDKATDPSLREEDWSLILDICDLINETETGPKEAVRLIKKRInspnphvallaltlldacvkncgskFHLEIASKEFLNE 85

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 237902000  154 L--RVAATDSVSDPDVREKCKQLFPQWAVTYKSTPGMERVALLYKQLPTR 201
Cdd:pfam00790  86 LvkLIPNKPYGKPAKVKKKILELIQEWADAFKNDPDLKYIRDMYKLLKRK 135
VHS smart00288
Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.
 99-202 1.05e-09

Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.


Pssm-ID: 197630 [Multi-domain]  Cd Length: 133  Bit Score: 56.54  E-value: 1.05e-09
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000    99 IDRLTSEQYDVDDSSGIVDLIEVIRLQSSGPQEASRALRKKL-------------------------FLKTFADEPLLER 153
Cdd:smart00288   5 IDKATSPSLLEEDWELILEICDLINSTPDGPKDAVRLLKKRLnnknphvallaltlldacvkncgskFHLEVASKEFLNE 84

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*....
gi 237902000   154 LRVAATDSVSDPDVREKCKQLFPQWAVTYKSTPGMERVALLYKQLPTRK 202
Cdd:smart00288  85 LVKLIKPKYPLPLVKKRILELIQEWADAFKNDPDLSQIVDVYDLLKKKG 133
GAT pfam03127
GAT domain; The GAT domain is responsible for binding of GGA proteins to several members of ...
 312-354 3.79e-05

GAT domain; The GAT domain is responsible for binding of GGA proteins to several members of the ARF family including ARF1 and ARF3. The GAT domain stabilizes membrane bound ARF1 in its GTP bound state, by interfering with GAP proteins.


Pssm-ID: 460818  Cd Length: 76  Bit Score: 41.76  E-value: 3.79e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 237902000  312 DVMEKF-ERCKSLRRQILRYI-QVVESDDFLGSLIHANEELVNAL 354
Cdd:pfam03127  23 ELIKELyERCKSAQPKIQKLIeETSDEDDLLAELLQLNDDLNNVL 67
 
Name Accession Description Interval E-value
GAT_LSB5 cd14232
canonical GAT domain found in yeast LAS seventeen-binding protein 5 (Lsb5p) and similar ...
 283-360 8.26e-40

canonical GAT domain found in yeast LAS seventeen-binding protein 5 (Lsb5p) and similar proteins; Lsb5p, also called LAS17-binding protein 5, is a Golgi-localized gamma ear-containing Arf-binding protein (GGA)-like protein located to the plasma membrane in an actin-independent manner. It plays important roles in membrane-trafficking events through association with the actin regulators, the yeast Wiskott-Aldrich syndrome protein (WASP) homologue Las17p and the cortical protein Sla1p, the yeast Arf3p (orthologous with mammalian Arf6), and ubiquitin. Lsb5p contains an N-terminal VHS (Vps27p/Hrs/STAM)-domain and a GAT (GGA and TOM1) domain. In contrast to GGA proteins, Lsb5p harbors a C-terminal NPF (Asn-Pro-Phe) motif, but does not have either a GAE (gamma-adaptin ear homology) domain or a clathrin-binding motif. The canonical GAT domain is a monomeric three-helix bundle that binds ubiquitin.


Pssm-ID: 410580  Cd Length: 78  Bit Score: 137.69  E-value: 8.26e-40
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 237902000 283 IASASVASTNLTNALKLVNREAQRVSENSDVMEKFERCKSLRRQILRYIQVVESDDFLGSLIHANEELVNALMAFEVL 360
Cdd:cd14232    1 IADASQAATNLVNALQLVNRETERVSDDAEVQERFEKCKQLRRKILRYIQLVEDEEYLGSLLHANEELVAALEAYDEL 78
VHS_Lsb5 cd16980
VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein ...
 95-199 3.24e-32

VHS (Vps27/Hrs/STAM) domain of LAS seventeen-binding protein 5; LAS seventeen-binding protein 5 (LAS17-binding protein 5, Lsb5, or Lsb5p) localizes to the plasma membrane and plays a role in endocytosis in yeast. It interacts with actin regulators Sla1p and Las17p, ubiquitin, and Arf3p, coupling actin dynamics to membrane trafficking processes. Lsb5p contains an N-terminal VHS domain and a GAT (GGA and TOM) domain. The VHS domain has a superhelical structure similar to the structure of ARM (Armadillo) repeats. It is a right-handed superhelix of eight alpha helices. The VHS domain has been found in a number of proteins, some of which have been implicated in intracellular trafficking and sorting.


Pssm-ID: 340777  Cd Length: 132  Bit Score: 119.24  E-value: 3.24e-32
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000  95 VTVQIDRLTSEQYDVDDSSGIVDLIEVIRLQSSGPQEASRALRKKL-------------------------FLKTFA-DE 148
Cdd:cd16980    1 ITDTIERLTSSEYEEEDWSLVLELCELINLIESGAKEAARALRKKLkygnphqqlraltlldllvenggkkFQAQFAsDK 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 237902000 149 PLLERLRVAATDSVSDPDVREKCKQLFPQWAVTYKSTPGMERVALLYKQLP 199
Cdd:cd16980   81 KLEDLLRLIASDPSTDPKVRKKLLELLGGWAYEFKGDPGLSGIASLYKKVK 131
VHS pfam00790
VHS domain; Domain present in VPS-27, Hrs and STAM.
 99-201 2.29e-11

VHS domain; Domain present in VPS-27, Hrs and STAM.


Pssm-ID: 395638  Cd Length: 136  Bit Score: 61.16  E-value: 2.29e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000   99 IDRLTSEQYDVDDSSGIVDLIEVIRLQSSGPQEASRALRKKL-------------------------FLKTFADEPLLER 153
Cdd:pfam00790   6 IDKATDPSLREEDWSLILDICDLINETETGPKEAVRLIKKRInspnphvallaltlldacvkncgskFHLEIASKEFLNE 85

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 237902000  154 L--RVAATDSVSDPDVREKCKQLFPQWAVTYKSTPGMERVALLYKQLPTR 201
Cdd:pfam00790  86 LvkLIPNKPYGKPAKVKKKILELIQEWADAFKNDPDLKYIRDMYKLLKRK 135
VHS smart00288
Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.
 99-202 1.05e-09

Domain present in VPS-27, Hrs and STAM; Unpublished observations. Domain of unknown function.


Pssm-ID: 197630 [Multi-domain]  Cd Length: 133  Bit Score: 56.54  E-value: 1.05e-09
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000    99 IDRLTSEQYDVDDSSGIVDLIEVIRLQSSGPQEASRALRKKL-------------------------FLKTFADEPLLER 153
Cdd:smart00288   5 IDKATSPSLLEEDWELILEICDLINSTPDGPKDAVRLLKKRLnnknphvallaltlldacvkncgskFHLEVASKEFLNE 84

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*....
gi 237902000   154 LRVAATDSVSDPDVREKCKQLFPQWAVTYKSTPGMERVALLYKQLPTRK 202
Cdd:smart00288  85 LVKLIKPKYPLPLVKKRILELIQEWADAFKNDPDLSQIVDVYDLLKKKG 133
VHS_Vps27 cd16979
VHS (Vps27/Hrs/STAM) domain of Vacuolar protein sorting-associated protein 27; Vacuolar ...
 98-198 3.16e-06

VHS (Vps27/Hrs/STAM) domain of Vacuolar protein sorting-associated protein 27; Vacuolar protein sorting-associated protein 27 (Vps27 or Vps27p) is also called Golgi retention defective protein 11, and is the yeast homolog of Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate). Together with class E vacuolar protein-sorting machinery protein HSE1, it comprises the ESCRT-0 complex, the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB). The complex directly binds to ubiquitinated transmembrane proteins and recruits both ubiquitin ligases and deubiquitinating enzymes. It is also required the efficient recycling of late Golgi proteins including the carboxypeptidase Y (CPY) sorting receptor, Vps10. Vps27 contain similar domains and motifs to Hrs; it contains an N-terminal VHS domain, which has a superhelical structure similar to the structure of ARM (Armadillo) repeats, a FYVE (Fab1p, YOTB, Vac1p, and EEA1) zinc finger domain, two Ubiquitin-Interacting Motifs (UIMs), a GAT (GGA and TOM) domain, two a P(S/T)XP motifs that recruit ESCRT-I, and a short peptide motif near the C-terminus that recruits clathrin.


Pssm-ID: 340776  Cd Length: 141  Bit Score: 46.42  E-value: 3.16e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000  98 QIDRLTSE-----QYDVDDSSGIVDLIeviRLQSSGPQEASRALRKKL-------------------------FLKTFAD 147
Cdd:cd16979    6 LIEKATSEsipngEEDLALALEISDLI---RSKSVPPKDAMRSLKKRIlhknpnvqllalkltdvcvknggdhFLVEIAS 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 237902000 148 EPLLERLRVAATDSVSDPDVREKCKQLFPQWAVTYKSTPGMERVALLYKQL 198
Cdd:cd16979   83 REFMDNLVSLIRSTGVNPEVKEKILRLIQEWALAFKSKSQLSYVVEVYKKL 133
VHS cd03561
VHS (Vps27/Hrs/STAM) domain family; The VHS domain is present in Vps27 (Vacuolar Protein ...
 99-198 1.48e-05

VHS (Vps27/Hrs/STAM) domain family; The VHS domain is present in Vps27 (Vacuolar Protein Sorting), Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and STAM (Signal Transducing Adaptor Molecule). It has a superhelical structure similar to that of the ARM (Armadillo) repeats and is present at the N-termini of proteins involved in intracellular membrane trafficking. There are four general groups of VHS domain containing proteins based on their association with other domains. The first group consists of proteins of the STAM/EAST/Hbp family, which has the domain composition of VHS-SH3-ITAM. The second consists of proteins with a FYVE domain C-terminal to VHS. The third consists of GGA proteins with a domain composition of VHS-GAT (GGA and TOM)-GAE (Gamma-Adaptin Ear) domain. The fourth consists of proteins with a VHS domain alone or with domains other than those mentioned above. In GGA proteins, VHS domains are involved in cargo recognition in trans-Golgi, thereby having a general membrane targeting/cargo recognition role in vesicular trafficking.


Pssm-ID: 340765  Cd Length: 131  Bit Score: 44.56  E-value: 1.48e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000  99 IDRLTSEQYDVDDSSGIVDLIEVIRLQSSGPQEASRALRKKL-------------------------FLKTFADEPLLER 153
Cdd:cd03561    5 VEKATSESLTEPDWALNLEICDLVNSDPAQAKDAVRALRKRLqsknpkvqllaltlletlvkncgapFHSEVASRDFLQE 84

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*
gi 237902000 154 LRVAATDSVSDPDVREKCKQLFPQWAVTYKSTPGMERVALLYKQL 198
Cdd:cd03561   85 LVKLVKKKKTSPEVREKALALIQEWAEAFQGDGRLPLFYDTYQQL 129
GAT pfam03127
GAT domain; The GAT domain is responsible for binding of GGA proteins to several members of ...
 312-354 3.79e-05

GAT domain; The GAT domain is responsible for binding of GGA proteins to several members of the ARF family including ARF1 and ARF3. The GAT domain stabilizes membrane bound ARF1 in its GTP bound state, by interfering with GAP proteins.


Pssm-ID: 460818  Cd Length: 76  Bit Score: 41.76  E-value: 3.79e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 237902000  312 DVMEKF-ERCKSLRRQILRYI-QVVESDDFLGSLIHANEELVNAL 354
Cdd:pfam03127  23 ELIKELyERCKSAQPKIQKLIeETSDEDDLLAELLQLNDDLNNVL 67
GAT_GGA_Tom1-like cd21383
canonical GAT domain found in eukaryotic ADP-ribosylation factor (Arf)-binding proteins (GGAs), ...
 299-360 1.50e-04

canonical GAT domain found in eukaryotic ADP-ribosylation factor (Arf)-binding proteins (GGAs), metazoan myb protein 1 (Tom1)-like proteins, and similar proteins; This model represents the canonical GAT (GGA and Tom1) domain found in GGAs from eukaryotes, Tom1-like proteins from metazoa, and LAS seventeen-binding protein 5 (Lsb5p)-like proteins from fungi. The canonical GAT domain is a monomeric three-helix bundle that binds ubiquitin. GGAs, also called Golgi-localized gamma-ear-containing Arf-binding proteins, belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. GGAs play important roles in ubiquitin-dependent sorting of cargo proteins both in biosynthetic and endocytic pathways. Tom1 and its related proteins, Tom1L1 and Tom1L2, form a protein family sharing an N-terminal VHS-domain followed by a GAT domain. Tom1 family proteins bind to ubiquitin, ubiquitinated proteins, and Toll-interacting protein (Tollip) through its GAT domain. They do not associate with either Arf GTPases through its GAT domain nor with acidic cluster-dileucine sequences through its VHS domain. In addition, Tom1 family proteins recruit clathrin onto endosomes through their C-terminal region. The C-terminal clathrin-binding region of Tom1 and Tom1L2 are similar to each other, but distinguishable from Tom1L1.


Pssm-ID: 410588  Cd Length: 80  Bit Score: 40.32  E-value: 1.50e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 237902000 299 LVNREAQRVSENSDVMEKFERCKSLRRQILRYIQVVE---SDDFLGSLIHANEELVNALMAFEVL 360
Cdd:cd21383   16 LSAASPEELLDNELLQELVEFCRACQPRIVELIEATTdglDEDTLEELLELNDELNEALQRYDDL 80
VHS_STAM cd03568
VHS (Vps27/Hrs/STAM) domain of the STAM (Signal Transducing Adaptor Molecule) subfamily; STAM ...
 99-198 7.17e-04

VHS (Vps27/Hrs/STAM) domain of the STAM (Signal Transducing Adaptor Molecule) subfamily; STAM (Signal Transducing Adaptor Molecule) subfamily members have at their N-termini a VHS domain, which is involved in cytokine-mediated intracellular signal transduction and has a superhelical structure similar to the structure of ARM (Armadillo) repeats, followed by a Ubiquitin-Interacting Motif (UIM) and a SH3 (Src Homology 3) domain, which is a well-established protein-protein interaction domain, and a GAT (GGA and TOM) domain. At the C-termini of most vertebrate STAMs, an Immunoreceptor Tyrosine-based Activation Motif (ITAM) is present, which mediates the binding of HRS (hepatocyte growth factor-regulated tyrosine kinase substrate) in endocytic and exocytic machineries. STAM is a component of the ESCRT (Endosomal Sorting Complex Required for Transport)-0 machinery and together with Hrs, functions to bind and sequester cargoes for downstream sorting into intralumenal vesicles. Jawed vertebrates have two STAM subfamily members, STAM1 and STAM2.


Pssm-ID: 340770  Cd Length: 132  Bit Score: 39.58  E-value: 7.17e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 237902000  99 IDRLTSEQYDVDDSSGIVDLIEVIRLQSSGPQEASRALRKKLFLKtfadEP--------LLE----------RLRVAATD 160
Cdd:cd03568    5 VEKATSEKNTSENWSLILDICDKVGRSPNGAKDCLKSIMKRLNHK----DPhvalqaltLLDacvkncgkifHLEVCSRD 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 237902000 161 SVSD---------PDVREKCKQLFPQWAVTYKST-PGMERVALLYKQL 198
Cdd:cd03568   81 FESElrklltkshPKVAEKLKELLKKWAEEEFKDdPQLSLIPSLYKKL 128
GAT_TM1L1 cd14237
canonical GAT domain found in target of Myb-like protein 1 (Tom1L1); Tom1L1, also called ...
 308-358 2.45e-03

canonical GAT domain found in target of Myb-like protein 1 (Tom1L1); Tom1L1, also called Src-activating and signaling molecule protein (Srcasm), was identified as a substrate of the Src family of protein kinases. It is tyrosine-phosphorylated by Src family kinases and modulates growth factor and Src-mediated signaling pathways. It also plays a potential role in endosomal sorting and ligand-stimulated endocytosis of EGF receptors (EGFR). Tom1L1 is predominantly present in the cytosol and can interact with Toll-interacting protein (Tollip), Hrs or TSG101, clathrin, and ubiquitinated proteins. It contains an N-terminal VHS (Vps27p/Hrs/STAM)-domain, a GAT (GGA and TOM1) domain, and a C-terminal clathrin-binding region, both of which are conserved in Golgi-localized gamma ear-containing Arf-binding proteins (GGAs). It interacts with Tollip through their GAT domain and recuits clathrin onto endosomes through their C-terminal region. However, in the C-terminal clathrin-binding region, Tom1 and Tom1L2 are similar to each other, but distinguishable from Tom1L1. The canonical GAT domain is a monomeric three-helix bundle that binds ubiquitin.


Pssm-ID: 410584  Cd Length: 92  Bit Score: 37.16  E-value: 2.45e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 237902000 308 SENSDVMEKFER----CKSLRRQILRYIQVVESDDFLGSLIHANEELVNALMAFE 358
Cdd:cd14237   32 AENPEDMELLEKlyktCREMQERIMKLLETVENEDVIIELIQVNDDLNNVFLRHE 86
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH