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Conserved domains on  [gi|2293759164|ref|NP_001398710|]
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calcium and integrin-binding family member 3 isoform 1 [Mus musculus]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 12144783)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

CATH:  1.10.238.10
Gene Ontology:  GO:0005509
PubMed:  2479149|10191494

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
107-174 1.06e-08

EF-hand domain pair;


:

Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 49.56  E-value: 1.06e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2293759164 107 KAYYAFKIYDFNNDNYICAWDLEQTVTRLTRG-ELSAEEVtlvcEKVLDEADGDQDGRLSLEDFQNMIL 174
Cdd:pfam13499   3 KLKEAFKLLDSDGDGYLDVEELKKLLRKLEEGePLSDEEV----EELFKEFDLDKDGRISFEEFLELYS 67
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
107-174 1.06e-08

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 49.56  E-value: 1.06e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2293759164 107 KAYYAFKIYDFNNDNYICAWDLEQTVTRLTRG-ELSAEEVtlvcEKVLDEADGDQDGRLSLEDFQNMIL 174
Cdd:pfam13499   3 KLKEAFKLLDSDGDGYLDVEELKKLLRKLEEGePLSDEEV----EELFKEFDLDKDGRISFEEFLELYS 67
PTZ00184 PTZ00184
calmodulin; Provisional
 57-175 1.68e-07

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 48.60  E-value: 1.68e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2293759164  57 ELIGSMPELKDNPFRQRIAQVFSQ---DGDGHMTLENFLDMFSvmSEMAPRDLKAYY--AFKIYDFNNDNYICAWDLEQT 131
Cdd:PTZ00184   32 ELGTVMRSLGQNPTEAELQDMINEvdaDGNGTIDFPEFLTLMA--RKMKDTDSEEEIkeAFKVFDRDGNGFISAAELRHV 109

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 2293759164 132 VTRLtrGE-LSAEEVtlvcEKVLDEADGDQDGRLSLEDFQNMILR 175
Cdd:PTZ00184  110 MTNL--GEkLTDEEV----DEMIREADVDGDGQINYEEFVKMMMS 148
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 111-173 4.39e-07

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 45.23  E-value: 4.39e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2293759164 111 AFKIYDFNNDNYICAWDLEQTVTRLTRGELSAEevtlvCEKVLDEADGDQDGRLSLEDFQNMI 173
Cdd:cd00051     5 AFRLFDKDGDGTISADELKAALKSLGEGLSEEE-----IDEMIREVDKDGDGKIDFEEFLELM 62
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
81-169 1.07e-05

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 43.24  E-value: 1.07e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2293759164  81 DGDGHMTLENFL-DMFSVMSEMAPRDLKAyyAFKIYDFNNDNYIcawDLEQTVTRLTRGELSAEEVtlvcEKVLDEADGD 159
Cdd:COG5126    45 DGDGRISREEFVaGMESLFEATVEPFARA--AFDLLDTDGDGKI---SADEFRRLLTALGVSEEEA----DELFARLDTD 115

                          90
                  ....*....|
gi 2293759164 160 QDGRLSLEDF 169
Cdd:COG5126   116 GDGKISFEEF 125
 
Name Accession Description Interval E-value
EF-hand_7 pfam13499
EF-hand domain pair;
107-174 1.06e-08

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 49.56  E-value: 1.06e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2293759164 107 KAYYAFKIYDFNNDNYICAWDLEQTVTRLTRG-ELSAEEVtlvcEKVLDEADGDQDGRLSLEDFQNMIL 174
Cdd:pfam13499   3 KLKEAFKLLDSDGDGYLDVEELKKLLRKLEEGePLSDEEV----EELFKEFDLDKDGRISFEEFLELYS 67
PTZ00184 PTZ00184
calmodulin; Provisional
 57-175 1.68e-07

calmodulin; Provisional


Pssm-ID: 185504 [Multi-domain]  Cd Length: 149  Bit Score: 48.60  E-value: 1.68e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2293759164  57 ELIGSMPELKDNPFRQRIAQVFSQ---DGDGHMTLENFLDMFSvmSEMAPRDLKAYY--AFKIYDFNNDNYICAWDLEQT 131
Cdd:PTZ00184   32 ELGTVMRSLGQNPTEAELQDMINEvdaDGNGTIDFPEFLTLMA--RKMKDTDSEEEIkeAFKVFDRDGNGFISAAELRHV 109

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 2293759164 132 VTRLtrGE-LSAEEVtlvcEKVLDEADGDQDGRLSLEDFQNMILR 175
Cdd:PTZ00184  110 MTNL--GEkLTDEEV----DEMIREADVDGDGQINYEEFVKMMMS 148
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 111-173 4.39e-07

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 45.23  E-value: 4.39e-07
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2293759164 111 AFKIYDFNNDNYICAWDLEQTVTRLTRGELSAEevtlvCEKVLDEADGDQDGRLSLEDFQNMI 173
Cdd:cd00051     5 AFRLFDKDGDGTISADELKAALKSLGEGLSEEE-----IDEMIREVDKDGDGKIDFEEFLELM 62
PTZ00183 PTZ00183
centrin; Provisional
 55-177 1.06e-05

centrin; Provisional


Pssm-ID: 185503 [Multi-domain]  Cd Length: 158  Bit Score: 43.52  E-value: 1.06e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2293759164  55 PYELIGSMPELKDNPFRQRIAQVFSQ---DGDGHMTLENFLDMFSV-MSEMAPRD--LKAyyaFKIYDFNNDNYICAWDL 128
Cdd:PTZ00183   36 PKELKVAMRSLGFEPKKEEIKQMIADvdkDGSGKIDFEEFLDIMTKkLGERDPREeiLKA---FRLFDDDKTGKISLKNL 112

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 2293759164 129 EQTVTRLtrGE-LSAEEVtlvcEKVLDEADGDQDGRLSLEDFQNMILRAP 177
Cdd:PTZ00183  113 KRVAKEL--GEtITDEEL----QEMIDEADRNGDGEISEEEFYRIMKKTN 156
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
81-169 1.07e-05

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 43.24  E-value: 1.07e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2293759164  81 DGDGHMTLENFL-DMFSVMSEMAPRDLKAyyAFKIYDFNNDNYIcawDLEQTVTRLTRGELSAEEVtlvcEKVLDEADGD 159
Cdd:COG5126    45 DGDGRISREEFVaGMESLFEATVEPFARA--AFDLLDTDGDGKI---SADEFRRLLTALGVSEEEA----DELFARLDTD 115

                          90
                  ....*....|
gi 2293759164 160 QDGRLSLEDF 169
Cdd:COG5126   116 GDGKISFEEF 125
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
71-173 7.12e-05

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 40.93  E-value: 7.12e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2293759164  71 RQRIAQVFS---QDGDGHMTLENFLDMFSVMSEMAprdlkayyaFKIYDFNNDNYIcawDLEQTVTRLTRGELSAEEVTL 147
Cdd:COG5126     4 RRKLDRRFDlldADGDGVLERDDFEALFRRLWATL---------FSEADTDGDGRI---SREEFVAGMESLFEATVEPFA 71

                          90       100
                  ....*....|....*....|....*.
gi 2293759164 148 vcEKVLDEADGDQDGRLSLEDFQNMI 173
Cdd:COG5126    72 --RAAFDLLDTDGDGKISADEFRRLL 95
EFh_PI-PLCeta cd16205
EF-hand motif found in phosphoinositide phospholipase C eta (PI-PLC-eta); PI-PLC-eta isozymes ...
 57-178 2.10e-03

EF-hand motif found in phosphoinositide phospholipase C eta (PI-PLC-eta); PI-PLC-eta isozymes represent a class of neuron-specific metazoan PI-PLCs that are most abundant in the brain, particularly in the hippocampus, habenula, olfactory bulb, cerebellum, and throughout the cerebral cortex. They are phosphatidylinositol 4,5-bisphosphate-hydrolyzing enzymes that are more sensitive to Ca2+ than other PI-PLC isozymes. They function as calcium sensors activated by small increases in intracellular calcium concentrations. They are also activated through G-protein-coupled receptor (GPCR) stimulation, and further mediate GPCR signalling pathways. PI-PLC-eta isozymes contain an N-terminal pleckstrin homology (PH) domain, four atypical EF-hand motifs, a PLC catalytic core domain, a C2 domain, and a unique C-terminal tail that terminates with a PDZ-binding motif, a potential interaction site for other signaling proteins. The PLC catalytic core domain is a TIM barrel with two highly conserved regions (X and Y) split by a highly degenerate linker sequence. The C-terminal tail harbors a number of proline-rich motifs which may interact with SH3 (Src homology 3) domain-containing proteins, as well as many serine/threonine residues, suggesting possible regulation of interactions by protein kinases/phosphatases. There are two PI-PLC-eta isozymes (1-2). Aside from the PI-PLC-eta isozymes identified in mammals, their eukaryotic homologs are also present in this family.


Pssm-ID: 320035 [Multi-domain]  Cd Length: 141  Bit Score: 36.98  E-value: 2.10e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2293759164  57 ELIGSMPELKDNPFRQRIAQVF----SQDGDGHMTLENFLDMFSVMSemAPRDLkaYYAFKIYDfNNDNYICAWDLeqtv 132
Cdd:cd16205    21 EILQLMHKLNVNLPRRKVRQMFkeadTDDNQGTLDFEEFCAFYKMMS--TRREL--YLLLLSYS-NKKDYLTLEDL---- 91

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 2293759164 133 TRLTRGELSAEEVTL-----VCEKVLDEADGDQDGRLSLEDFQNMiLRAPD 178
Cdd:cd16205    92 ARFLEVEQKMTNVTLeycldIIEKFEPSEENKKNGLLGIDGFTNY-MRSPA 141
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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