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Conserved domains on  [gi|1099848601|emb|SFH25179|]
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Acetyltransferase (GNAT) domain-containing protein [Lachnospiraceae bacterium NLAE-zl-G231]

Protein Classification

GNAT family N-acetyltransferase( domain architecture ID 12153134)

GNAT family N-acetyltransferase catalyzes the transfer of an acetyl group from acetyl-CoA to a substrate; similar to Bacillus subtilis acetyltransferase BSU40680

CATH:  3.40.630.30
EC:  2.3.1.-
Gene Ontology:  GO:0008080
PubMed:  10940244|9175471|15581578
SCOP:  3000403

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 27-153 9.98e-36

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


:

Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 120.07  E-value: 9.98e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601  27 VAPDYTQEGIDNFYqEYVHGAGFRNKFTDKREVMYGAFVGNNLAGVLSFSDKNTVSCVFVRGKYHKMGIGRCLFEKAdEE 106
Cdd:pfam13673   1 EAPDYSEEGIETFY-EFISPEALRERIDQGEYFFFVAFEGGQIVGVIALRDRGHISLLFVDPDYQGQGIGKALLEAV-ED 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1099848601 107 MCSR---GADKIKLNASPYAVPFYHHLGFQDTGAQAVYKGIIYTPMELLL 153
Cdd:pfam13673  79 YAEKdgiKLSELTVNASPYAVPFYEKLGFRATGPEQEFNGIRFVPMEKEL 128
 
Name Accession Description Interval E-value
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 27-153 9.98e-36

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 120.07  E-value: 9.98e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601  27 VAPDYTQEGIDNFYqEYVHGAGFRNKFTDKREVMYGAFVGNNLAGVLSFSDKNTVSCVFVRGKYHKMGIGRCLFEKAdEE 106
Cdd:pfam13673   1 EAPDYSEEGIETFY-EFISPEALRERIDQGEYFFFVAFEGGQIVGVIALRDRGHISLLFVDPDYQGQGIGKALLEAV-ED 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1099848601 107 MCSR---GADKIKLNASPYAVPFYHHLGFQDTGAQAVYKGIIYTPMELLL 153
Cdd:pfam13673  79 YAEKdgiKLSELTVNASPYAVPFYEKLGFRATGPEQEFNGIRFVPMEKEL 128
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
61-153 4.24e-13

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 62.12  E-value: 4.24e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601  61 YGAFVGNNLAGVLSF--SDKNTVSC--VFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLNASPYAVPFYHHLGFQDTG 136
Cdd:COG2153    37 LLAYDDGELVATARLlpPGDGEAKIgrVAVLPEYRGQGLGRALMEAAIEEARERGARRIVLSAQAHAVGFYEKLGFVPVG 116

                          90
                  ....*....|....*..
gi 1099848601 137 AQAVYKGIIYTPMELLL 153
Cdd:COG2153   117 EEFLEAGIPHIDMRKPL 133
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 60-118 1.35e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.03  E-value: 1.35e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1099848601  60 MYGAFVGNNLAGVLSFSDKNT------VSCVFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLN 118
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGSggdtayIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
Spm_actase_Thplmales NF041158
spermidine N(1)-acetyltransferase;
1-107 5.94e-03

spermidine N(1)-acetyltransferase;


Pssm-ID: 469070  Cd Length: 115  Bit Score: 34.90  E-value: 5.94e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601   1 MIEIRLLDDKQTEEAFSLIWEVFLEFVAPDYTQEGIDNFYQEYVHGAGFRNKFTDKRE----VMYGAFVGNNLAGVLSF- 75
Cdd:NF041158    1 MITIRKLSAEDVDALIEVARESWKWTYRDIYSNEFIESWISEKYSKEKLLNEIIRSQSnldiIFLGAFVNSALIGFIELk 80

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1099848601  76 --SDKNTVSCVFVRGKYHKMGIGRCLFEKADEEM 107
Cdd:NF041158   81 iiADKAELLRLYLKPEYTHRGIGKLLLSEAEKIM 114
 
Name Accession Description Interval E-value
Acetyltransf_10 pfam13673
Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase ...
 27-153 9.98e-36

Acetyltransferase (GNAT) domain; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 463953 [Multi-domain]  Cd Length: 128  Bit Score: 120.07  E-value: 9.98e-36
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601  27 VAPDYTQEGIDNFYqEYVHGAGFRNKFTDKREVMYGAFVGNNLAGVLSFSDKNTVSCVFVRGKYHKMGIGRCLFEKAdEE 106
Cdd:pfam13673   1 EAPDYSEEGIETFY-EFISPEALRERIDQGEYFFFVAFEGGQIVGVIALRDRGHISLLFVDPDYQGQGIGKALLEAV-ED 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 1099848601 107 MCSR---GADKIKLNASPYAVPFYHHLGFQDTGAQAVYKGIIYTPMELLL 153
Cdd:pfam13673  79 YAEKdgiKLSELTVNASPYAVPFYEKLGFRATGPEQEFNGIRFVPMEKEL 128
ElaA COG2153
Predicted N-acyltransferase, GNAT family [General function prediction only];
61-153 4.24e-13

Predicted N-acyltransferase, GNAT family [General function prediction only];


Pssm-ID: 441756 [Multi-domain]  Cd Length: 134  Bit Score: 62.12  E-value: 4.24e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601  61 YGAFVGNNLAGVLSF--SDKNTVSC--VFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLNASPYAVPFYHHLGFQDTG 136
Cdd:COG2153    37 LLAYDDGELVATARLlpPGDGEAKIgrVAVLPEYRGQGLGRALMEAAIEEARERGARRIVLSAQAHAVGFYEKLGFVPVG 116

                          90
                  ....*....|....*..
gi 1099848601 137 AQAVYKGIIYTPMELLL 153
Cdd:COG2153   117 EEFLEAGIPHIDMRKPL 133
ArgA COG1246
N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and ...
 55-155 2.59e-09

N-acetylglutamate synthase or related acetyltransferase, GNAT family [Amino acid transport and metabolism]; N-acetylglutamate synthase or related acetyltransferase, GNAT family is part of the Pathway/BioSystem: Arginine biosynthesis


Pssm-ID: 440859 [Multi-domain]  Cd Length: 132  Bit Score: 52.30  E-value: 2.59e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601  55 DKREVMYGAFVGNNLAGV--LSFSDKNT--VSCVFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLNASPYAVPFYHHL 130
Cdd:COG1246    25 EEIGEFWVAEEDGEIVGCaaLHPLDEDLaeLRSLAVHPDYRGRGIGRRLLEALLAEARELGLKRLFLLTTSAAIHFYEKL 104

                          90       100
                  ....*....|....*....|....*
gi 1099848601 131 GFQDTGAQAVYKGIIYTPMELLLKK 155
Cdd:COG1246   105 GFEEIDKEDLPYAKVWQRDSVVMEK 129
yhbS COG3153
Predicted N-acetyltransferase YhbS [General function prediction only];
4-153 5.73e-09

Predicted N-acetyltransferase YhbS [General function prediction only];


Pssm-ID: 442387 [Multi-domain]  Cd Length: 142  Bit Score: 51.62  E-value: 5.73e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601   4 IRLLDDKQTEEAFSLIWEVFlefvAPDYTQEGIDNFYQEYVHGAGFrnkftdkrevmyGAFVGNNLAGVLSFSDKN---- 79
Cdd:COG3153     1 IRPATPEDAEAIAALLRAAF----GPGREAELVDRLREDPAAGLSL------------VAEDDGEIVGHVALSPVDidge 64

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1099848601  80 ----TVSCVFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLNASPYAVPFYHHLGFQDTGAQAVYKGIIYTPMELLL 153
Cdd:COG3153    65 gpalLLGPLAVDPEYRGQGIGRALMRAALEAARERGARAVVLLGDPSLLPFYERFGFRPAGELGLTLGPDEVFLAKEL 142
PhnO COG0454
N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, ...
 84-146 8.56e-08

N-acetyltransferase, GNAT superfamily (includes histone acetyltransferase HPA2) [Transcription, General function prediction only];


Pssm-ID: 440222 [Multi-domain]  Cd Length: 136  Bit Score: 48.13  E-value: 8.56e-08
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1099848601  84 VFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLNASPY---AVPFYHHLGFQDTGAQAVYKGIIY 146
Cdd:COG0454    64 LYVLPEYRGKGIGKALLEALLEWARERGCTALELDTLDGnpaAIRFYERLGFKEIERYVAYVGGEF 129
RimI COG0456
Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal ...
 81-155 2.21e-07

Ribosomal protein S18 acetylase RimI and related acetyltransferases [Translation, ribosomal structure and biogenesis];


Pssm-ID: 440224 [Multi-domain]  Cd Length: 92  Bit Score: 46.19  E-value: 2.21e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1099848601  81 VSCVFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLN---ASPYAVPFYHHLGFQDTGAQAVYkgiiYTPMELLLKK 155
Cdd:COG0456    16 IEDLAVDPEYRGRGIGRALLEAALERARERGARRLRLEvreDNEAAIALYEKLGFEEVGERPNY----YGDDALVMEK 89
Acetyltransf_7 pfam13508
Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.
 56-133 1.05e-06

Acetyltransferase (GNAT) domain; This domain catalyzes N-acetyltransferase reactions.


Pssm-ID: 463905 [Multi-domain]  Cd Length: 84  Bit Score: 44.37  E-value: 1.05e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601  56 KREVMYGAFVGNNLAGVLSFSDKNTVSC-----VFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLNASPYAVPFYHHL 130
Cdd:pfam13508   1 PGGRFFVAEDDGKIVGFAALLPLDDEGAlaelrLAVHPEYRGQGIGRALLEAAEAAAKEGGIKLLELETTNRAAAFYEKL 80


                  ...
gi 1099848601 131 GFQ 133
Cdd:pfam13508  81 GFE 83
Acetyltransf_1 pfam00583
Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase ...
 20-132 2.00e-05

Acetyltransferase (GNAT) family; This family contains proteins with N-acetyltransferase functions such as Elp3-related proteins.


Pssm-ID: 395465 [Multi-domain]  Cd Length: 116  Bit Score: 41.35  E-value: 2.00e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601  20 WEVFLEFVAPDYTQEGIDNFYQEyvhgagFRNKFTDKREVMYGAFVGNNLAGVLSFSDKNT------VSCVFVRGKYHKM 93
Cdd:pfam00583   1 LEALYELLSEEFPEPWPDEPLDL------LEDWDEDASEGFFVAEEDGELVGFASLSIIDDeppvgeIEGLAVAPEYRGK 74

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 1099848601  94 GIGRCLFEKADEEMCSRGADKIKLNASP---YAVPFYHHLGF 132
Cdd:pfam00583  75 GIGTALLQALLEWARERGCERIFLEVAAdnlAAIALYEKLGF 116
NAT_SF cd04301
N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer ...
 60-118 1.35e-04

N-Acyltransferase superfamily: Various enzymes that characteristically catalyze the transfer of an acyl group to a substrate; NAT (N-Acyltransferase) is a large superfamily of enzymes that mostly catalyze the transfer of an acyl group to a substrate and are implicated in a variety of functions, ranging from bacterial antibiotic resistance to circadian rhythms in mammals. Members include GCN5-related N-Acetyltransferases (GNAT) such as Aminoglycoside N-acetyltransferases, Histone N-acetyltransferase (HAT) enzymes, and Serotonin N-acetyltransferase, which catalyze the transfer of an acetyl group to a substrate. The kinetic mechanism of most GNATs involves the ordered formation of a ternary complex: the reaction begins with Acetyl Coenzyme A (AcCoA) binding, followed by binding of substrate, then direct transfer of the acetyl group from AcCoA to the substrate, followed by product and subsequent CoA release. Other family members include Arginine/ornithine N-succinyltransferase, Myristoyl-CoA: protein N-myristoyltransferase, and Acyl-homoserinelactone synthase which have a similar catalytic mechanism but differ in types of acyl groups transferred. Leucyl/phenylalanyl-tRNA-protein transferase and FemXAB nonribosomal peptidyltransferases which catalyze similar peptidyltransferase reactions are also included.


Pssm-ID: 173926 [Multi-domain]  Cd Length: 65  Bit Score: 38.03  E-value: 1.35e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1099848601  60 MYGAFVGNNLAGVLSFSDKNT------VSCVFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLN 118
Cdd:cd04301     1 FLVAEDDGEIVGFASLSPDGSggdtayIGDLAVLPEYRGKGIGSALLEAAEEEARERGAKRLRLE 65
MnaT COG1247
L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];
1-136 6.55e-04

L-amino acid N-acyltransferase MnaT [Amino acid transport and metabolism];


Pssm-ID: 440860 [Multi-domain]  Cd Length: 163  Bit Score: 38.05  E-value: 6.55e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601   1 MIEIRLLddkqTEEAFSLIWEVFLEFVApdytqEGIDNFYQEYVHGAGFRNKFTDKREVMYGAFV---GNNLAGVLSFSD 77
Cdd:COG1247     1 EMTIRPA----TPEDAPAIAAIYNEAIA-----EGTATFETEPPSEEEREAWFAAILAPGRPVLVaeeDGEVVGFASLGP 71

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601  78 KNTVSC--------VFVRGKYHKMGIGRCLFEKADEEMCSRGADKIKLNASPY---AVPFYHHLGFQDTG 136
Cdd:COG1247    72 FRPRPAyrgtaeesIYVDPDARGRGIGRALLEALIERARARGYRRLVAVVLADneaSIALYEKLGFEEVG 141
Spm_actase_Thplmales NF041158
spermidine N(1)-acetyltransferase;
1-107 5.94e-03

spermidine N(1)-acetyltransferase;


Pssm-ID: 469070  Cd Length: 115  Bit Score: 34.90  E-value: 5.94e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1099848601   1 MIEIRLLDDKQTEEAFSLIWEVFLEFVAPDYTQEGIDNFYQEYVHGAGFRNKFTDKRE----VMYGAFVGNNLAGVLSF- 75
Cdd:NF041158    1 MITIRKLSAEDVDALIEVARESWKWTYRDIYSNEFIESWISEKYSKEKLLNEIIRSQSnldiIFLGAFVNSALIGFIELk 80

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1099848601  76 --SDKNTVSCVFVRGKYHKMGIGRCLFEKADEEM 107
Cdd:NF041158   81 iiADKAELLRLYLKPEYTHRGIGKLLLSEAEKIM 114
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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