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Conserved domains on  [gi|1275661557|ref|WP_099746301|]
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MULTISPECIES: Asp23/Gls24 family envelope stress response protein [Enterococcus]

Protein Classification

Asp23/Gls24 family envelope stress response protein( domain architecture ID 10510257)

Asp23/Gls24 family envelope stress response protein similar to Staphylococcus epidermidis alkaline shock protein 23 (Asp23) and Enterococcus hirae general stress protein Gls24

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Asp23 pfam03780
Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified ...
 24-131 5.32e-33

Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified as an alkaline shock protein that was expressed in a sigmaB-dependent manner in Staphylococcus aureus. Following an alkaline shock Asp23 accumulates in the soluble protein fraction of the S. aureus cell. Asp23 is one of the most abundant proteins in the cytosolic protein fraction of stationary S. aureus cells, with a copy-number of >25000 per cell. A second Asp23-family protein, AmaP, which is encoded within the asp23-operon, is required to localize Asp23 to the cell membrane. The overall function for the family is thus a cell envelope-related one in Gram-positive bacteria. UniProtKB:Q2FW64.


:

Pssm-ID: 427500  Cd Length: 108  Bit Score: 113.76  E-value: 5.32e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1275661557  24 MNGELTYEDKVIQKIIGYALKDVDGLLTLDGGFFSNMAEKLVNtDNVTAGINTEVG-KKQVAVDLDVIVEYGKDIEDIYN 102
Cdd:pfam03780   1 ELGEITIADEVIAKIAGIAATEVDGVVGMAGGLKDGLAERLGR-ENLTKGVKVEVGeEGQVAVDLYVIVEYGVSIPEVAE 79

                          90       100
                  ....*....|....*....|....*....
gi 1275661557 103 QIKELISTEVNNMTHLDVIEVNVNVADIK 131
Cdd:pfam03780  80 NIQENVKYAVETMTGLEVVEVNVHVQGVR 108
 
Name Accession Description Interval E-value
Asp23 pfam03780
Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified ...
 24-131 5.32e-33

Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified as an alkaline shock protein that was expressed in a sigmaB-dependent manner in Staphylococcus aureus. Following an alkaline shock Asp23 accumulates in the soluble protein fraction of the S. aureus cell. Asp23 is one of the most abundant proteins in the cytosolic protein fraction of stationary S. aureus cells, with a copy-number of >25000 per cell. A second Asp23-family protein, AmaP, which is encoded within the asp23-operon, is required to localize Asp23 to the cell membrane. The overall function for the family is thus a cell envelope-related one in Gram-positive bacteria. UniProtKB:Q2FW64.


Pssm-ID: 427500  Cd Length: 108  Bit Score: 113.76  E-value: 5.32e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1275661557  24 MNGELTYEDKVIQKIIGYALKDVDGLLTLDGGFFSNMAEKLVNtDNVTAGINTEVG-KKQVAVDLDVIVEYGKDIEDIYN 102
Cdd:pfam03780   1 ELGEITIADEVIAKIAGIAATEVDGVVGMAGGLKDGLAERLGR-ENLTKGVKVEVGeEGQVAVDLYVIVEYGVSIPEVAE 79

                          90       100
                  ....*....|....*....|....*....
gi 1275661557 103 QIKELISTEVNNMTHLDVIEVNVNVADIK 131
Cdd:pfam03780  80 NIQENVKYAVETMTGLEVVEVNVHVQGVR 108
YloU COG1302
Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function ...
 25-138 8.17e-33

Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function unknown];


Pssm-ID: 440913  Cd Length: 118  Bit Score: 113.34  E-value: 8.17e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1275661557  25 NGELTYEDKVIQKIIGYALKDVDGLLTLDGGFFSNMAEKLvNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYNQI 104
Cdd:COG1302     6 LGKITIADEVIAKIAGIAALEVYGVVGMAGGFADGIAELL-GRKNLTKGVKVEVGEDQVAIDLYVVVEYGVNIPEVAENV 84

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1275661557 105 KELISTEVNNMTHLDVIEVNVNVADIKTQEEYQK 138
Cdd:COG1302    85 QENVKYAVEKMTGLEVVEVNVHVQGVRFPEEEEE 118
anchor_AmaP NF033218
alkaline shock response membrane anchor protein AmaP; The founding member of this family, AmaP ...
 25-127 5.29e-04

alkaline shock response membrane anchor protein AmaP; The founding member of this family, AmaP (Asp23 membrane anchoring protein), is related to Asp23 through part of its length, but includes a highly hydrophobic N-terminal region that should make it an integral membrane protein. Asp23 (alkaline shock protein of 23 kDa), described in PMID:7864904, is a cytosolic protein in Staphylococcus aureus, strongly induced by a pH shift from 7 to 10, and also recruited to the membrane. AmaP appears to be the partner protein with an integral membrane segment and the ability to anchor Asp23 to the membrane. This model was built to identify full-length homologs of AmaP, while excluding Asp23. Some but not all members of this family score above the cutoffs of Pfam model version PF03780.11, but full-length homologs of Asp23 score considerably higher. Asp23 family members previously were known as DUF322.


Pssm-ID: 468002  Cd Length: 166  Bit Score: 38.72  E-value: 5.29e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1275661557  25 NGELTYEDKVIQKIIGYALKDVDGLltldggffsnmaeklvntDNVTagINTEVGKKQVAVDLDVIVEYGKDIEDIYNQI 104
Cdd:NF033218   84 DGKLTISKSAIEGFVREALSKVDGV------------------RNPK--VKVKLKKRKIKVDVKGDLDPRSNLPELTEEI 143

                          90       100
                  ....*....|....*....|...
gi 1275661557 105 KELISTEVNNMTHLDVIEVNVNV 127
Cdd:NF033218  144 QEEIKEGLEELLGIPVKKVKVKV 166
 
Name Accession Description Interval E-value
Asp23 pfam03780
Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified ...
 24-131 5.32e-33

Asp23 family, cell envelope-related function; The alkaline shock protein Asp23 was identified as an alkaline shock protein that was expressed in a sigmaB-dependent manner in Staphylococcus aureus. Following an alkaline shock Asp23 accumulates in the soluble protein fraction of the S. aureus cell. Asp23 is one of the most abundant proteins in the cytosolic protein fraction of stationary S. aureus cells, with a copy-number of >25000 per cell. A second Asp23-family protein, AmaP, which is encoded within the asp23-operon, is required to localize Asp23 to the cell membrane. The overall function for the family is thus a cell envelope-related one in Gram-positive bacteria. UniProtKB:Q2FW64.


Pssm-ID: 427500  Cd Length: 108  Bit Score: 113.76  E-value: 5.32e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1275661557  24 MNGELTYEDKVIQKIIGYALKDVDGLLTLDGGFFSNMAEKLVNtDNVTAGINTEVG-KKQVAVDLDVIVEYGKDIEDIYN 102
Cdd:pfam03780   1 ELGEITIADEVIAKIAGIAATEVDGVVGMAGGLKDGLAERLGR-ENLTKGVKVEVGeEGQVAVDLYVIVEYGVSIPEVAE 79

                          90       100
                  ....*....|....*....|....*....
gi 1275661557 103 QIKELISTEVNNMTHLDVIEVNVNVADIK 131
Cdd:pfam03780  80 NIQENVKYAVETMTGLEVVEVNVHVQGVR 108
YloU COG1302
Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function ...
 25-138 8.17e-33

Uncharacterized conserved protein YloU, alkaline shock protein (Asp23) family [Function unknown];


Pssm-ID: 440913  Cd Length: 118  Bit Score: 113.34  E-value: 8.17e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1275661557  25 NGELTYEDKVIQKIIGYALKDVDGLLTLDGGFFSNMAEKLvNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYNQI 104
Cdd:COG1302     6 LGKITIADEVIAKIAGIAALEVYGVVGMAGGFADGIAELL-GRKNLTKGVKVEVGEDQVAIDLYVVVEYGVNIPEVAENV 84

                          90       100       110
                  ....*....|....*....|....*....|....
gi 1275661557 105 KELISTEVNNMTHLDVIEVNVNVADIKTQEEYQK 138
Cdd:COG1302    85 QENVKYAVEKMTGLEVVEVNVHVQGVRFPEEEEE 118
anchor_AmaP NF033218
alkaline shock response membrane anchor protein AmaP; The founding member of this family, AmaP ...
 25-127 5.29e-04

alkaline shock response membrane anchor protein AmaP; The founding member of this family, AmaP (Asp23 membrane anchoring protein), is related to Asp23 through part of its length, but includes a highly hydrophobic N-terminal region that should make it an integral membrane protein. Asp23 (alkaline shock protein of 23 kDa), described in PMID:7864904, is a cytosolic protein in Staphylococcus aureus, strongly induced by a pH shift from 7 to 10, and also recruited to the membrane. AmaP appears to be the partner protein with an integral membrane segment and the ability to anchor Asp23 to the membrane. This model was built to identify full-length homologs of AmaP, while excluding Asp23. Some but not all members of this family score above the cutoffs of Pfam model version PF03780.11, but full-length homologs of Asp23 score considerably higher. Asp23 family members previously were known as DUF322.


Pssm-ID: 468002  Cd Length: 166  Bit Score: 38.72  E-value: 5.29e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1275661557  25 NGELTYEDKVIQKIIGYALKDVDGLltldggffsnmaeklvntDNVTagINTEVGKKQVAVDLDVIVEYGKDIEDIYNQI 104
Cdd:NF033218   84 DGKLTISKSAIEGFVREALSKVDGV------------------RNPK--VKVKLKKRKIKVDVKGDLDPRSNLPELTEEI 143

                          90       100
                  ....*....|....*....|...
gi 1275661557 105 KELISTEVNNMTHLDVIEVNVNV 127
Cdd:NF033218  144 QEEIKEGLEELLGIPVKKVKVKV 166
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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