FhaA, N-terminal domain; This domain can be found at the N terminus of FHA domain-containing protein Fha from Mycobacterium tuberculosis. The FHA (Fork Head Associated) domain is a phosphopeptide- specific binding domain that enables the protein to specifically bind to sequences containing phosphothreonine. FhaA is one of the major substrates of Ser/Thr protein kinases (STPK) and plays a role in the regulation of cell growth and peptidoglycan synthesis by binding to MviN. It may inhibit the late stages of peptidoglycan synthesis.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873   2 ERKLEATVDNAFARVFGGPIVPQEVEALLCREARDGVQKLHGNRLLAPNEYIITLGMHDFEKVSADPDLTSSAFARYLAD 81
Cdd:pfam12401   5 ERRLERAVEGAFARVFGGEVQPVEIAKALRREMDDNARVVSGGRVLAPNVYTVELSPDDYERLAPWGDTLADELADYLAE 84

                          90       100       110
                  ....*....|....*....|....*....|
gi 2181016873  82 YIHEQGWQTYGEVVVRFEQSSSLRTGQYRA 111
Cdd:pfam12401  85 HAREQGYTFVGPVVVRFEEDESLHTGQFRV 114

FhaA, N-terminal domain; This domain can be found at the N terminus of FHA domain-containing protein Fha from Mycobacterium tuberculosis. The FHA (Fork Head Associated) domain is a phosphopeptide- specific binding domain that enables the protein to specifically bind to sequences containing phosphothreonine. FhaA is one of the major substrates of Ser/Thr protein kinases (STPK) and plays a role in the regulation of cell growth and peptidoglycan synthesis by binding to MviN. It may inhibit the late stages of peptidoglycan synthesis.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873   2 ERKLEATVDNAFARVFGGPIVPQEVEALLCREARDGVQKLHGNRLLAPNEYIITLGMHDFEKVSADPDLTSSAFARYLAD 81
Cdd:pfam12401   5 ERRLERAVEGAFARVFGGEVQPVEIAKALRREMDDNARVVSGGRVLAPNVYTVELSPDDYERLAPWGDTLADELADYLAE 84

                          90       100       110
                  ....*....|....*....|....*....|
gi 2181016873  82 YIHEQGWQTYGEVVVRFEQSSSLRTGQYRA 111
Cdd:pfam12401  85 HAREQGYTFVGPVVVRFEEDESLHTGQFRV 114

FHA domain; The FHA (Forkhead-associated) domain is a phosphopeptide binding motif.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2181016873 463 IIGRGQDAQFRLPDTGVSRRHLEIRWDGQVAL-LSDLNSTNGTTVNNAPVQE--WQLADGDVIRL 524
Cdd:pfam00498   2 TIGRSPDCDIVLDDPSVSRRHAEIRYDGGGRFyLEDLGSTNGTFVNGQRLGPepVRLKDGDVIRL 66

type VI secretion system FHA domain protein; Members of this protein family are FHA (forkhead-associated) domain-containing proteins that are part of type VI secretion loci in a considerable number of bacteria, most of which are known pathogens. Species include Pseudomonas aeruginosa PAO1, Aeromonas hydrophila, Yersinia pestis, Burkholderia mallei, etc. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 446 QLDDGSGRTYQLREGSNIIGRGQDAQFRLPDTG--VSRRHLEIRWDGQVALLSDLnSTNGTTVNNAPVQ-----EWQLAD 518
Cdd:TIGR03354  10 QLTPGIAAQKTFGTNGGTIGRSEDCDWVLPDPErhVSGRHARIRYRDGAYLLTDL-STNGVFLNGSGSPlgrgnPVRLEQ 88

                          90
                  ....*....|....*.
gi 2181016873 519 GDVIRLGHSEIIVRIH 534
Cdd:TIGR03354  89 GDRLRLGDYEIRVSLG 104

FhaA, N-terminal domain; This domain can be found at the N terminus of FHA domain-containing protein Fha from Mycobacterium tuberculosis. The FHA (Fork Head Associated) domain is a phosphopeptide- specific binding domain that enables the protein to specifically bind to sequences containing phosphothreonine. FhaA is one of the major substrates of Ser/Thr protein kinases (STPK) and plays a role in the regulation of cell growth and peptidoglycan synthesis by binding to MviN. It may inhibit the late stages of peptidoglycan synthesis.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873   2 ERKLEATVDNAFARVFGGPIVPQEVEALLCREARDGVQKLHGNRLLAPNEYIITLGMHDFEKVSADPDLTSSAFARYLAD 81
Cdd:pfam12401   5 ERRLERAVEGAFARVFGGEVQPVEIAKALRREMDDNARVVSGGRVLAPNVYTVELSPDDYERLAPWGDTLADELADYLAE 84

                          90       100       110
                  ....*....|....*....|....*....|
gi 2181016873  82 YIHEQGWQTYGEVVVRFEQSSSLRTGQYRA 111
Cdd:pfam12401  85 HAREQGYTFVGPVVVRFEEDESLHTGQFRV 114

forkhead associated (FHA) domain superfamily; Forkhead-associated (FHA) domains are small phosphopeptide recognition modules mostly found in eubacteria and eukaryotes. It is about 95-120 residues long that fold into an 11-stranded beta-sandwich. FHA domains can mediate the recognition of phosphorylated and non-phosphorylated substrates, as well as protein oligomerization. They specifically recognize threonine phosphorylation (pThr) accompanying activation of protein serine/threonine kinases. FHA domains show diverse ligand specificity. They may recognize the pTXXD motif, the pTXXI/L motif, and TQ clusters (singly and multiply phosphorylated). In eukaryotes, FHA superfamily members include forkhead-type transcription factors, as well as other signaling proteins, such as many regulatory proteins, kinases, phosphatases, motor proteins called kinesins, and metabolic enzymes. Many of them localize to the nucleus, where they participate in establishing or maintaining cell cycle checkpoints, DNA repair, or transcriptional regulation. FHA domains play important roles in human diseases, particularly in relation to DNA damage responses and cancers. In bacteria, FHA domain-containing proteins may participate in injection of viral proteins into host cells, transmembrane transporters, and cell division. FHA domain-containing proteins rarely include more than one copy of the domain. The only exception in eukaryotes is the checkpoint kinase Rad53 from Saccharomyces cerevisiae, which harbors two FHA domains (FHA1 and FHA2) flanking a central kinase domain. The two FHA domains recognize different phosphorylated targets and function independently from one another. In contrast, Mycobacterium tuberculosis ABC transporter Rv1747 contains two FHA domains but only one of them is essential for protein function.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 448 DDGSGRTYQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLSDLNSTNGTTVNNAPVQEW-QLADGDVIRLGH 526
Cdd:cd00060     7 GDGGGREFPLTKGVVTIGRSPDCDIVLDDPSVSRRHARIEVDGGGVYLEDLGSTNGTFVNGKRITPPvPLQDGDVIRLGD 86

                  ....*.
gi 2181016873 527 SEIIVR 532
Cdd:cd00060    87 TTFRFE 92

FHA domain; The FHA (Forkhead-associated) domain is a phosphopeptide binding motif.

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2181016873 463 IIGRGQDAQFRLPDTGVSRRHLEIRWDGQVAL-LSDLNSTNGTTVNNAPVQE--WQLADGDVIRL 524
Cdd:pfam00498   2 TIGRSPDCDIVLDDPSVSRRHAEIRYDGGGRFyLEDLGSTNGTFVNGQRLGPepVRLKDGDVIRL 66

forkhead associated (FHA) domain found in proliferation marker protein Ki-67 and similar proteins; Ki-67, also called antigen identified by monoclonal antibody Ki-67, antigen KI-67, or antigen Ki67, acts as a biological surfactant to disperse mitotic chromosomes. It is required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Ki-67 binds DNA with a preference for supercoiled DNA and AT-rich DNA. It may also play a role in chromatin organization. Ki-67 contains an FHA domain at its N-terminus. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 443 VTLQLDDGSGRTYQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQV-ALLSDLNSTNGTTVNNAPVQE-WQLADGD 520
Cdd:cd22673     4 VVIKKDGTDGSRFPLTKKSCTFGRDLSCDIRIQLPGVSREHCRIEVDENGkAYLENLSTTNPTLVNGKAIEKsAELKDGD 83

                  ....*
gi 2181016873 521 VIRLG 525
Cdd:cd22673    84 VITIG 88

Predicted component of the type VI protein secretion system, contains a FHA domain [Signal transduction mechanisms, Intracellular trafficking, secretion, and vesicular transport];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 446 QLDDGSGRTYQLREGSNIIGRGQDAQFRLPDTG--VSRRHLEIRWDGQVALLSDLnSTNGTTVNNAPV-----QEWQLAD 518
Cdd:COG3456    12 DLESGSAASATFGRGGGTIGRSADCDWVLPDPDrsVSRRHAEIRFRDGAFCLTDL-STNGTFLNGSDHplgpgRPVRLRD 90

                          90
                  ....*....|....*
gi 2181016873 519 GDVIRLGHSEIIVRI 533
Cdd:COG3456    91 GDRLRIGDYEIRVEI 105

forkhead associated (FHA) domain found in Mycobacterium tuberculosis FHA domain-containing protein FhaB and similar proteins; FhaB, also called FtsZ-interacting protein A (FipA), is a putative virulence factor involved in regulating cell shape. It can interact with polyketide-associated protein PapA5, a putative membrane protein involved in the biosynthesis of virulence enhancing lipids. FhaB regulates growth and cell division. It is probably required for divisomal protein assembly under oxidative stress. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 452 GRTYQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLSDLNSTNGTTVNNAPV-QEWQLADGDVIRLGHSEII 530
Cdd:cd22693    10 GQTFPIDKSGITIGRADDNDLVLSDDFVSSRHARIYLQGSSWYLEDLGSTNGTFVNGNRVtQPVVVQPGDTIRIGATVFE 89

                  ..
gi 2181016873 531 VR 532
Cdd:cd22693    90 VR 91

forkhead associated (FHA) domain found in Arabidopsis thaliana protein phosphatase 2C 70 (AtPP2C70) and similar proteins; AtPP2C70, also called kinase-associated protein phosphatase, or protein ROOT ATTENUATED GROWTH 1, dephosphorylates the serine/threonine receptor-like kinase RLK5. It may function as a signaling component in a pathway involving RLK5. It acts as a negative regulator of the CLAVATA1 signaling in plant development by binding and dephosphorylating CLAVATA1. It is also a component of a signaling pathway which mediates adaptation to NaCl stress. It contains an FHA domain, which is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 451 SGRTYQLREGSN---IIGRGQDAQFRLPDTGVSRRHLEIRWDGQVA--LLSDLNSTNGTTVNNAPVQ----EWQLADGDV 521
Cdd:cd22678    11 EGLRFDLQPGTRlplTIGRIQRGDIALKDDEVSGKHARIEWNSTGSkwELVDLGSLNGTLVNGESISpngrPVVLSSGDV 90

                          90
                  ....*....|...
gi 2181016873 522 IRLGhSEIIVRIH 534
Cdd:cd22678    91 ITLG-SETKILVR 102

type VI secretion system FHA domain protein; Members of this protein family are FHA (forkhead-associated) domain-containing proteins that are part of type VI secretion loci in a considerable number of bacteria, most of which are known pathogens. Species include Pseudomonas aeruginosa PAO1, Aeromonas hydrophila, Yersinia pestis, Burkholderia mallei, etc. [Protein fate, Protein and peptide secretion and trafficking, Cellular processes, Pathogenesis]

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 446 QLDDGSGRTYQLREGSNIIGRGQDAQFRLPDTG--VSRRHLEIRWDGQVALLSDLnSTNGTTVNNAPVQ-----EWQLAD 518
Cdd:TIGR03354  10 QLTPGIAAQKTFGTNGGTIGRSEDCDWVLPDPErhVSGRHARIRYRDGAYLLTDL-STNGVFLNGSGSPlgrgnPVRLEQ 88

                          90
                  ....*....|....*.
gi 2181016873 519 GDVIRLGHSEIIVRIH 534
Cdd:TIGR03354  89 GDRLRLGDYEIRVSLG 104

second forkhead associated (FHA) domain found in Mycobacterium tuberculosis ABC transporter ATP-binding/permease protein Rv1747 and similar proteins; Rv1747 is a putative ATP-binding cassette (ABC) transporter involved in the translocation of an unknown substrate across the membrane. It is required for normal virulent infection by M. tuberculosis. Rv1747 has a cytoplasmic regulatory module consisting of two pThr-interacting forkhead-associated (FHA) domains connected by a conformationally disordered linker with two phospho-acceptor threonines (pThr). Recruitment and phosphorylation of Rv1747 depend on the interaction between its two non-redundant FHA domains and the autophosphorylated form of serine/threonine protein kinase PknF. This model corresponds to the second FHA domain, which has a circularly permuted FHA domain fold with a conserved pThr-binding interface.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 449 DGSGRTYQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLSDLNSTNGTTVNNAPVQEWQLADGDVIRLGHSE 528
Cdd:cd22737    10 DGDTLTFELPPQAVRIGRASDNDIVIPEGSVSRHHATLVPTPGGTQIRDLRSTNGTFVNGLRVDAALLHDGDVVTIGDID 89

                  .
gi 2181016873 529 I 529
Cdd:cd22737    90 F 90

forkhead associated (FHA) domain found in Saccharomyces cerevisiae meiosis-specific serine/threonine-protein kinase MEK1 and similar proteins; MEK1 (EC 2.7.11.1), also known as MRE4, is a meiosis-specific protein kinase required for chromosome synapsis and meiotic recombination. The recruitment and activation of MEK1 require the phosphorylation of the chromosome axis protein Hop1 at Thr318 (pT318), which is necessary for recognition by the MEK1 FHA domain. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 457 LREGSNI-IGRGQDAQFRLPDTGVSRRHLEI---RWDGQVALL---SDLnSTNGTTVNNAPVQEWQ---LADGDVIRLGH 526
Cdd:cd22670    18 IYKNQVItIGRSPSCDIVINDPFVSRTHCRIysvQFDESSAPLvyvEDL-SSNGTYLNGKLIGRNNtvlLSDGDVIEIAH 96

                  ....*...
gi 2181016873 527 SEIIVRIH 534
Cdd:cd22670    97 SATFVYVH 104

forkhead associated (FHA) domain found in Chlamydia trachomatis Ct664 protein and similar proteins; This subfamily corresponds to a group of uncharacterized FHA domain-containing proteins which show high sequence similarity with Chlamydia trachomatis Ct664 protein. Ct664 situates within the type III secretion system cluster that also encodes an STPK (CT673 in C. trachomatis), suggesting a role of CT664 in the chlamydial type III secretion system by mediating phosphorylation-dependent protein-protein interactions. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 451 SGRTYqlregsnIIGR-GQDAQFRLPDTGVSRRH--LEIRWDGQVaLLSDLNSTNGTTVNNAPVQEWQLADG-DVIRLGH 526
Cdd:cd22696    19 SGKTY-------FIGKdPTVCDIVLQDPSISRQHarLSIDQDNRV-FIEDLSSKNGVLVNGKPIEGKEEISGsDVISLGT 90

                  ....*
gi 2181016873 527 SEIIV 531
Cdd:cd22696    91 TSFLL 95

forkhead associated (FHA) domain found in nibrin and similar proteins; Nibrin (NBN), also called cell cycle regulatory protein p95, or Nijmegen breakage syndrome protein 1 (NBS1), is a novel DNA double-strand break repair protein that is mutated in Nijmegen breakage syndrome. It is a component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. Nibrin modulates the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. Nibrin also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. Nibrin is a major player in the control of intra-S-phase checkpoint. This subfamily also includes Schizosaccharomyces pombe DNA repair and telomere maintenance protein Nbs1 and Arabidopsis thaliana AtNbs1. SpNbs1 is an FHA domain-containing protein required for DNA damage repair and S-phase DNA damage checkpoint. It is involved in telomere length maintenance and maintenance of chromatin structure. AtNbs1 is a component of MRN complex. It also functions in the very early stages of meiosis. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 448 DDGSGRTYQLREG-SNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLSDLN----------STNGTTVNNAPVQEWQ- 515
Cdd:cd22667     7 QDGAGTSYYLLPGgEYTVGRKDCDIIIVDDSSISRKHATLTVLHPEANLSDPDtrpeltlkdlSKYGTFVNGEKLKGGSe 86

                          90       100
                  ....*....|....*....|.
gi 2181016873 516 --LADGDVIRLGHSEIIVRIH 534
Cdd:cd22667    87 vtLKDGDVITFGVLGSKFRVE 107

forkhead associated (FHA) domain found in Arabidopsis thaliana Protein PARALLEL SPINDLE 1 (PS1) and similar proteins; PS1 is an FHA domain-containing protein required for normal spindle orientation at male meiosis II and normal formation of tetrad of microspores. It is not involved in female meiosis. Mutations in PS1 lead to the production of diploid pollen grains. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2181016873 463 IIGRGQDAQFRLPDTGVSRRHLEIRWDG--QVALLSDLNSTNGTTVNN---APVQEWQLADGDVIRLGHSEIIVRIH 534
Cdd:cd22691    32 VVGRHPDCDIVLDHPSISRFHLEIRIIPsrRKITLTDLSSVHGTWVNGqriEPGVPVELEEGDTVRLGASTRVYRLH 108

forkhead associated (FHA) domain found in Synechocystis sp. Slr1951 protein and similar proteins; This subfamily corresponds to a group of uncharacterized FHA domain-containing proteins which show high sequence similarity with Synechocystis sp. protein Slr1951 and protein Sll1895. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 443 VTLQLDDGSGRTYQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVAL----------LSDLNSTNGTTVNNAPVQ 512
Cdd:cd22697     1 ILVELDGNVRREIRLDEPIYTIGRHPGNDIQIPSQQISRRHATLRRKINPNLdisfwiidgdLEGAESLNGLWVNGERIL 80

                          90       100
                  ....*....|....*....|..
gi 2181016873 513 EWQLADGDVIRLGHSeIIVRIH 534
Cdd:cd22697    81 QHELVNGDEIALGPK-IVLRYQ 101

forkhead associated (FHA) domain found in Corynebacterium glutamicum oxoglutarate dehydrogenase inhibitor (OdhI) and similar proteins; OdhI is an essential component of the PknG signaling pathway. It regulates glutamate production under biotin non-limiting conditions. It can inhibit the activity of 2-oxoglutarate dehydrogenase only when it is unphosphorylated. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 451 SGRTYQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLSDLNSTNGTTVNNAPVQEWQLADGDVIRLGHSEII 530
Cdd:cd22721    20 AGARFLLDQPTTTAGRHPESDIFLDDVTVSRRHAEFRINEGEFEVVDVGSLNGTYVNREPRNAQVMQTGDEIQIGKFRLV 99

forkhead associated (FHA) domain found in Smad nuclear-interacting protein 1 (SNIP1), FHA domain-containing protein DDL, and similar proteins; SNIP1 is an FHA domain-containing protein required for pre-mRNA splicing as a component of the spliceosome. It inhibits NF-kappaB signaling by competing for its binding to the C/H1 domain of CBP/p300 transcriptional co-activators. It is involved in microRNA (miRNA) biogenesis. SNIP1 is a regulator of the cell cycle and cyclin D1 expression and may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. This family also includes Arabidopsis thaliana FHA domain-containing protein DDL and similar proteins. DDL, also called protein DAWDLE, is involved in the microRNA (miRNA) and short interfering RNA (siRNA) biogenesis. It may facilitate DCL1 to access or recognize primary miRNAs. DDL binds RNA non-specifically. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30
                  ....*....|....*....|....*....|....*
gi 2181016873 497 DLNSTNGTTVNNAPVQEW---QLADGDVIRLGHSE 528
Cdd:cd22676    71 DLGSTNGTFLNGEKIEPRryyELREKDVLKFGLST 105

chromosomal replication initiator protein DnaA;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 110 RACGavnPDVQPRPTVDDPVRPQSnnafgeergvAPMTDNSSYRGAQGPGRPGDEYydERYGRPQDDARGGSEPQG--AP 187
Cdd:PRK14086   76 RELG---RPIRIAITVDPSAGEPA----------PPPPHARRTSEPELPRPGRRPY--EGYGGPRADDRPPGLPRQdqLP 140

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 188 DQRGGYPPEQAGYPPQQ--GYPPPRHPEQ--GGYPEQGGYPPPQSYQEH-----GGYPDQRGGYPEP-GQGGYPPQQHGY 257
Cdd:PRK14086  141 TARPAYPAYQQRPEPGAwpRAADDYGWQQqrLGFPPRAPYASPASYAPEqerdrEPYDAGRPEYDQRrRDYDHPRPDWDR 220

                         170       180
                  ....*....|....*....|....*....
gi 2181016873 258 PDQ----RGYPEPAQGGYPQSYEQRPPAP 282
Cdd:PRK14086  221 PRRdrtdRPEPPPGAGHVHRGGPGPPERD 249

forkhead associated (FHA) domain found in Saccharomyces cerevisiae fork head protein homolog 1 (FKH1), 2 (FKH2) and similar proteins; This family includes FKH1 and FKH2, as well as pre-rRNA-processing protein FHL1. FKH1 and FKH2 are forkhead transcription factors that regulate the expression of the CLB2 cluster of genes during the G2/M phase of the mitotic cell cycle. The CLB2 cluster of genes includes mitotic regulators such as CLB1, CLB2, CDC5 and CDC20, as well as SWI5 and ACE2. FKH1 and FKH2 are involved in HMRa silencing. They associate with the coding regions of active genes and influence, in opposing ways, transcriptional elongation and termination, and coordinate early transcription elongation and pre-mRNA processing. Both FKH1 and FKH2 play a role as regulators of lifespan in collaboration with the anaphase-promoting complex (APC), likely through combined regulation of stress response, genomic stability, and cell cycle regulation. They also function in controlling yeast cell morphology by preventing pseudohyphal growth and act as rate-limiting replication origin activators via their interaction with the origin recognition complex (ORC). FHL1 is a forkhead protein that controls the pre-rRNA processing machinery in conjunction with IFH1. It might act as a transcriptional regulator of genes specifically involved in that process. IFH1 convert FHL1 from a repressor to an activator. This family also includes AtFHA1 and AtFHA2, which may play a role in the control of plant organ development. AtFHA2 is specifically involved in the regulation of stamen development. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 449 DGSGRTYQLREGSNIIGR-----------GQDAQFRlPDTGVSRRHLEIRWD---GQVALLSdlNSTNGTTVNNAPV--- 511
Cdd:cd22701     6 DGDDFTYYVQKLEVVLGRnsknssstaadSVDIDLG-PSKKISRRHARIFYDfttQCFELSV--LGRNGVKVDGILVkpg 82

                          90
                  ....*....|....*
gi 2181016873 512 -QEWQLADGDVIRLG 525
Cdd:cd22701    83 sPPVPLRSGSLIQIG 97

second forkhead associated (FHA) domain found in Saccharomyces cerevisiae Serine/threonine-protein kinase RAD53 and similar proteins; RAD53, also called CHEK2 homolog, or serine-protein kinase 1 (Spk1), is a nuclear protein kinase that phosphorylates proteins on serine, threonine, and tyrosine. It controls S-phase checkpoint as well as G1 and G2 DNA damage checkpoints and prevents entry into anaphase and mitotic exit after DNA damage via regulation of the Polo kinase CDC5. It may be involved in the phosphorylation of RPH1. RAD53 contains two FHA domains. This model corresponds to the second one. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 452 GRTYQ---LREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQ-----VALLSDlNSTNGTTVNNAPVQ--EWQ-LADGD 520
Cdd:cd22690     8 NPSYPdieLTQNTTFIGRSKDCDEEITDPRISKHHCIITRKRSgkgldDVYVTD-TSTNGTFINNNRLGkgSQSlLQDGD 86

                  ....
gi 2181016873 521 VIRL 524
Cdd:cd22690    87 EIVL 90

DNA polymerase III subunits gamma and tau; Validated

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 154 GAQGPGRPGDEYYDERYGRPQDDARGGSEPQGAPDQRGGYPPEQAGYPPQQGYPPPRHPEQGGYPEQGGYPPPQSyqeHG 233
Cdd:PRK07764  596 GGEGPPAPASSGPPEEAARPAAPAAPAAPAAPAPAGAAAAPAEASAAPAPGVAAPEHHPKHVAVPDASDGGDGWP---AK 672

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 234 GYPDQRGGYPEPGQGGYPPQQHGYPDQRGYPEPAQGGYPQSYEQRPPAPPGYS-GQGYDQGYRPPGGYPPPGGQPAGGPQ 312
Cdd:PRK07764  673 AGGAAPAAPPPAPAPAAPAAPAGAAPAQPAPAPAATPPAGQADDPAAQPPQAAqGASAPSPAADDPVPLPPEPDDPPDPA 752

                         170       180       190
                  ....*....|....*....|....*....|..
gi 2181016873 313 GYGGYGDYGRGPARPDEGGYAPPEQRPAYPEQ 344
Cdd:PRK07764  753 GAPAQPPPPPAPAPAAAPAAAPPPSPPSEEEE 784

forkhead associated (FHA) domain found in sarcolemmal membrane-associated protein (SLMAP) and similar proteins; SLMAP, also called sarcolemmal-associated protein (SLAP), is a tail-anchored protein involved in fundamental cellular processes, such as myoblast fusion, cell cycle progression, and chromosomal inheritance. It is a cardiac membrane protein that plays an important role in E-C coupling and the adrenergic response of the heart. Overexpression of the SLMAP gene has been associated with diabetes and endothelial dysfunction of macro- and micro-blood vessels. SLMAP contains an N-terminal FHA domain, which is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2181016873 480 SRRHLEIrW--DGQVaLLSDLNSTNGTTVNNA-------PVQEWQLADGDVIRLG-----------HSEIIVRIH 534
Cdd:cd22679    50 SRNHALL-WydDGKF-YLQDTKSSNGTFVNNQrlskgseESEPRELHSGDIVQFGvdvvensrkvtHGCIVATVT 122

forkhead associated (FHA) domain found in aprataxin and PNK-like factor (APLF) and similar proteins; APLF, also called apurinic-apyrimidinic endonuclease APLF, PNK and APTX-like FHA domain-containing protein, or XRCC1-interacting protein 1 (XIP1), is a novel apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease with conserved zinc-finger-like motifs involved in single-strand and double-strand DNA break repair. It is recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. It can introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, APLF promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). APLF contains an FHA domain at its N-terminus. The FHA domain is a small phosphopeptide recognition module.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2181016873 443 VTLQLDDGSGRTyQLREGSNIIGRGQdaQFRLPDTGVSRRH--LEIRwDGQVALLS-DLNSTNGTTVNNAPVQ-----EW 514
Cdd:cd22717     2 FELQPVDGGKRI-ELPPGETTIGRGP--FLGITDKRVSRNHaiLEVV-DGKLRIKPtHTNPCFYQPSGKSKLIplkkdEW 77

                          90       100
                  ....*....|....*....|.
gi 2181016873 515 Q-LADGDVIRLGHSEIIVRIH 534
Cdd:cd22717    78 QeLENGDSFSLLPDKLIFRVV 98