PA14 domain; This domain forms an insert in bacterial beta-glucosidases and is found in other glycosidases, glycosyltransferases, proteases, amidases, yeast adhesins, and bacterial toxins, including anthrax protective antigen (PA). The domain also occurs in a Dictyostelium prespore-cell-inducing factor Psi and in fibrocystin, the mammalian protein whose mutation leads to polycystic kidney and hepatic disease. The crystal structure of PA shows that this domain (named PA14 after its location in the PA20 pro-peptide) has a beta-barrel structure. The PA14 domain sequence suggests a binding function, rather than a catalytic role. The PA14 domain distribution is compatible with carbohydrate binding.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1023946303  120 IIAYWSTDLFGFYTTPTNVTLEMTGYFLPPQTGSYTFKFAtVDDSAILSVGGNVAFECCAQEQPPITSTdftisgikqwn 199
Cdd:pfam07691   28 NTFYWDTDVPGFGEAPGDFSARWTGYLLPPESGTYTFGVA-SDDGARLWIDGELVIDNWGQHPPDASPE----------- 95

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|..
gi 1023946303  200 esppdnITGTVYMYAGFYYPMKIVYSNAVAWGTLPISVTLPDGTTVSDDFEG 251
Cdd:pfam07691   96 ------ESNTLYLVAGKLYPIRIEYFHAGTGGSVQLSWTSPDGGGEEIDEDG 141

Flocculin type 3 repeat; This repeat is found in the Flocculation protein FLO9 close to its C-terminus.

                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....
gi 1023946303  965 TTLVTVTSCESGVCSETASPAIVSTATATVNDVVTVYPTWRPQT 1008
Cdd:pfam13928    1 TTVITITSCSDDKCHETTVTTGVSTVTTTVNGVVTVYTTYCPLT 44

PA14 domain; This domain forms an insert in bacterial beta-glucosidases and is found in other glycosidases, glycosyltransferases, proteases, amidases, yeast adhesins, and bacterial toxins, including anthrax protective antigen (PA). The domain also occurs in a Dictyostelium prespore-cell-inducing factor Psi and in fibrocystin, the mammalian protein whose mutation leads to polycystic kidney and hepatic disease. The crystal structure of PA shows that this domain (named PA14 after its location in the PA20 pro-peptide) has a beta-barrel structure. The PA14 domain sequence suggests a binding function, rather than a catalytic role. The PA14 domain distribution is compatible with carbohydrate binding.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1023946303  120 IIAYWSTDLFGFYTTPTNVTLEMTGYFLPPQTGSYTFKFAtVDDSAILSVGGNVAFECCAQEQPPITSTdftisgikqwn 199
Cdd:pfam07691   28 NTFYWDTDVPGFGEAPGDFSARWTGYLLPPESGTYTFGVA-SDDGARLWIDGELVIDNWGQHPPDASPE----------- 95

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|..
gi 1023946303  200 esppdnITGTVYMYAGFYYPMKIVYSNAVAWGTLPISVTLPDGTTVSDDFEG 251
Cdd:pfam07691   96 ------ESNTLYLVAGKLYPIRIEYFHAGTGGSVQLSWTSPDGGGEEIDEDG 141

Flocculin type 3 repeat; This repeat is found in the Flocculation protein FLO9 close to its C-terminus.

                           10        20        30        40
                   ....*....|....*....|....*....|....*....|..
gi 1023946303  844 LVTVTSCESHVCTESISSAIVSTATVTVSGATTEYTTWCPIS 885
Cdd:pfam13928    3 VITITSCSDDKCHETTVTTGVSTVTTTVNGVVTVYTTYCPLT 44

Flocculin type 3 repeat; This repeat is found in the Flocculation protein FLO9 close to its C-terminus.

                           10        20        30
                   ....*....|....*....|....*....|....*....
gi 1023946303  919 ISSCESDVCSKTASPAIVSISTATINGVTTEYTTWCPIS 957
Cdd:pfam13928    6 ITSCSDDKCHETTVTTGVSTVTTTVNGVVTVYTTYCPLT 44

PA14 domain; This domain forms an insert in bacterial beta-glucosidases and is found in other glycosidases, glycosyltransferases, proteases, amidases, yeast adhesins, and bacterial toxins, including anthrax protective antigen (PA). The domain also occurs in a Dictyostelium prespore-cell-inducing factor Psi and in fibrocystin, the mammalian protein whose mutation leads to polycystic kidney and hepatic disease. The crystal structure of PA shows that this domain (named PA14 after its location in the PA20 pro-peptide) has a beta-barrel structure. The PA14 domain sequence suggests a binding function, rather than a catalytic role. The PA14 domain distribution is compatible with carbohydrate binding.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1023946303  120 IIAYWSTDLFGFYTTPTNVTLEMTGYFLPPQTGSYTFKFAtVDDSAILSVGGNVAFECCAQEQPPITSTdftisgikqwn 199
Cdd:pfam07691   28 NTFYWDTDVPGFGEAPGDFSARWTGYLLPPESGTYTFGVA-SDDGARLWIDGELVIDNWGQHPPDASPE----------- 95

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|..
gi 1023946303  200 esppdnITGTVYMYAGFYYPMKIVYSNAVAWGTLPISVTLPDGTTVSDDFEG 251
Cdd:pfam07691   96 ------ESNTLYLVAGKLYPIRIEYFHAGTGGSVQLSWTSPDGGGEEIDEDG 141

GLEYA domain; The GLEYA domain is related to lectin-like binding domains found in the S. cerevisiae Flo proteins and the C. glabrata Epa proteins. It is a carbohydrate-binding domain that is found in fungal adhesins (also referred to as agglutinins or flocculins). Adhesins with a GLEYA domain possess a typical N-terminal signal peptide and a domain of conserved sequence repeats, but lack glycosylphosphatidylinositol (GPI) anchor attachment signals. They contain a conserved motif G(M/L)(E/A/N/Q)YA, hence the name GLEYA. Based on sequence homology, it is suggested that the GLEYA domain would predominantly contain beta sheets. The GLEYA domain is also found in S. pombe protein Swiss:Q92344, thought to be a kinetochore portein (Sim4 complex subunit), however no direct evidence for kinetochore association has been found. Furthermore, a global protein localization study in S. pombe identified it as a secreted protein localized to the Golgi complex.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1023946303  139 TLEMTGYFLPPQTGSYTFKFATVDDSAILSVGGNvAFECCAQEQPPITSTDFTISGIKQWnesppdnitgTVYMYAGFYY 218
Cdd:pfam10528    2 ALNHRGYFYAPVTGTYTFTLPNADDIAYLWLGDK-AYSGWTRANADLVATYGGGGGSGTY----------TVTLTAGTYY 70

                           90       100
                   ....*....|....*....|
gi 1023946303  219 PMKIVYSNAVAWGTLPISVT 238
Cdd:pfam10528   71 PIRIVYANGGGPGSFNFTIT 90

Flocculin type 3 repeat; This repeat is found in the Flocculation protein FLO9 close to its C-terminus.

                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....
gi 1023946303  965 TTLVTVTSCESGVCSETASPAIVSTATATVNDVVTVYPTWRPQT 1008
Cdd:pfam13928    1 TTVITITSCSDDKCHETTVTTGVSTVTTTVNGVVTVYTTYCPLT 44