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Conserved domains on  [gi|116242812|sp|P29083|]
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RecName: Full=General transcription factor IIE subunit 1; AltName: Full=General transcription factor IIE 56 kDa subunit; AltName: Full=Transcription initiation factor IIE subunit alpha; Short=TFIIE-alpha

Protein Classification

TFIIE and TFIIE-A_C domain-containing protein( domain architecture ID 12214403)

TFIIE and TFIIE-A_C domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TFIIE smart00531
Transcription initiation factor IIE;
28-175 3.15e-64

Transcription initiation factor IIE;


:

Pssm-ID: 128804 [Multi-domain]  Cd Length: 147  Bit Score: 203.37  E-value: 3.15e-64
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116242812    28 IEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETAADGKTtRHNYYF-INYRTLVNVVKYK 106
Cdd:smart00531   1 GEAFLVLDALMRNGCVTEEDLAELLGIKQKQLRKILYLLYDEKLIKIDYKREKDPETKT-WYRYYWyINYDTLLDVVKYK 79

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 116242812   107 LDHMRRRIETDERDSTNRASFKCPVCSSTFTDLEANQLFDpMTGTFRCTFCHTEVEEDESAMPKKDART 175
Cdd:smart00531  80 LDKMRKRLEDKLEDETNNAYYKCPNCQSKYTFLEANQLLD-MDGTFTCPRCGEELEEDDNSEPIEELRD 147
TFIIE-A_C pfam11521
C-terminal general transcription factor TFIIE alpha; TFIIE is compiled of two subunits, alpha ...
 394-439 2.29e-26

C-terminal general transcription factor TFIIE alpha; TFIIE is compiled of two subunits, alpha and beta. This family of proteins are the C terminal domain of the alpha subunit of the protein which is the largest subunit and contains several functional domains which are important for basal transcription and cell growth. The C terminal end of the protein binds directly to the amino-terminal PH domain of p62/Tfb1 (of IIH) which is involved in the recruitment of the general transcription factor IIH to the transcription preinitiation complex. P53 competes for the same binding site as TFIIE alpha which shows their structural similarity. Like p53, TFIIE alpha 336-439 can activate transcription in vivo.


:

Pssm-ID: 463287  Cd Length: 85  Bit Score: 101.64  E-value: 2.29e-26
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 116242812  394 PIVMVAGRPFSYSEVSQRPELVAQMTPEEKEAYIAMGQRMFEDLFE 439
Cdd:pfam11521  40 PTVMVAGRPVSYSEVSQRPELVAQMTPEEKEAYIKVGQQMFSDMYD 85
 
Name Accession Description Interval E-value
TFIIE smart00531
Transcription initiation factor IIE;
28-175 3.15e-64

Transcription initiation factor IIE;


Pssm-ID: 128804 [Multi-domain]  Cd Length: 147  Bit Score: 203.37  E-value: 3.15e-64
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116242812    28 IEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETAADGKTtRHNYYF-INYRTLVNVVKYK 106
Cdd:smart00531   1 GEAFLVLDALMRNGCVTEEDLAELLGIKQKQLRKILYLLYDEKLIKIDYKREKDPETKT-WYRYYWyINYDTLLDVVKYK 79

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 116242812   107 LDHMRRRIETDERDSTNRASFKCPVCSSTFTDLEANQLFDpMTGTFRCTFCHTEVEEDESAMPKKDART 175
Cdd:smart00531  80 LDKMRKRLEDKLEDETNNAYYKCPNCQSKYTFLEANQLLD-MDGTFTCPRCGEELEEDDNSEPIEELRD 147
TFIIE_alpha pfam02002
TFIIE alpha subunit; The general transcription factor TFIIE has an essential role in ...
 16-123 5.08e-44

TFIIE alpha subunit; The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits TFIIE-alpha and TFIIE-beta and joins the pre-initiation complex after RNA polymerase II and TFIIF. This family consists of the conserved amino terminal region of eukaryotic TFIIE-alpha and proteins from archaebacteria that are presumed to be TFIIE-alpha subunits also Swiss:O29501.


Pssm-ID: 280224  Cd Length: 105  Bit Score: 149.47  E-value: 5.08e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116242812   16 RLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVEtaaDGKTTRHNYYFIN 95
Cdd:pfam02002   1 ELLKEVVRGFYGEEAVLVLDVLLFNGEVTEEDLAELLKIDLNELRKLLNRLYEARLISYRRRRD---DETGWYRYYWRIN 77

                          90       100
                  ....*....|....*....|....*...
gi 116242812   96 YRTLVNVVKYKLDHMRRRIETDERDSTN 123
Cdd:pfam02002  78 YEKAIDVIKRKLHETVRRLEKDLEFETN 105
TFIIE-A_C pfam11521
C-terminal general transcription factor TFIIE alpha; TFIIE is compiled of two subunits, alpha ...
 394-439 2.29e-26

C-terminal general transcription factor TFIIE alpha; TFIIE is compiled of two subunits, alpha and beta. This family of proteins are the C terminal domain of the alpha subunit of the protein which is the largest subunit and contains several functional domains which are important for basal transcription and cell growth. The C terminal end of the protein binds directly to the amino-terminal PH domain of p62/Tfb1 (of IIH) which is involved in the recruitment of the general transcription factor IIH to the transcription preinitiation complex. P53 competes for the same binding site as TFIIE alpha which shows their structural similarity. Like p53, TFIIE alpha 336-439 can activate transcription in vivo.


Pssm-ID: 463287  Cd Length: 85  Bit Score: 101.64  E-value: 2.29e-26
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 116242812  394 PIVMVAGRPFSYSEVSQRPELVAQMTPEEKEAYIAMGQRMFEDLFE 439
Cdd:pfam11521  40 PTVMVAGRPVSYSEVSQRPELVAQMTPEEKEAYIKVGQQMFSDMYD 85
 
Name Accession Description Interval E-value
TFIIE smart00531
Transcription initiation factor IIE;
28-175 3.15e-64

Transcription initiation factor IIE;


Pssm-ID: 128804 [Multi-domain]  Cd Length: 147  Bit Score: 203.37  E-value: 3.15e-64
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116242812    28 IEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVETAADGKTtRHNYYF-INYRTLVNVVKYK 106
Cdd:smart00531   1 GEAFLVLDALMRNGCVTEEDLAELLGIKQKQLRKILYLLYDEKLIKIDYKREKDPETKT-WYRYYWyINYDTLLDVVKYK 79

                           90       100       110       120       130       140
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 116242812   107 LDHMRRRIETDERDSTNRASFKCPVCSSTFTDLEANQLFDpMTGTFRCTFCHTEVEEDESAMPKKDART 175
Cdd:smart00531  80 LDKMRKRLEDKLEDETNNAYYKCPNCQSKYTFLEANQLLD-MDGTFTCPRCGEELEEDDNSEPIEELRD 147
TFIIE_alpha pfam02002
TFIIE alpha subunit; The general transcription factor TFIIE has an essential role in ...
 16-123 5.08e-44

TFIIE alpha subunit; The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits TFIIE-alpha and TFIIE-beta and joins the pre-initiation complex after RNA polymerase II and TFIIF. This family consists of the conserved amino terminal region of eukaryotic TFIIE-alpha and proteins from archaebacteria that are presumed to be TFIIE-alpha subunits also Swiss:O29501.


Pssm-ID: 280224  Cd Length: 105  Bit Score: 149.47  E-value: 5.08e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 116242812   16 RLAKYVIRGFYGIEHALALDILIRNSCVKEEDMLELLKFDRKQLRSVLNNLKGDKFIKCRMRVEtaaDGKTTRHNYYFIN 95
Cdd:pfam02002   1 ELLKEVVRGFYGEEAVLVLDVLLFNGEVTEEDLAELLKIDLNELRKLLNRLYEARLISYRRRRD---DETGWYRYYWRIN 77

                          90       100
                  ....*....|....*....|....*...
gi 116242812   96 YRTLVNVVKYKLDHMRRRIETDERDSTN 123
Cdd:pfam02002  78 YEKAIDVIKRKLHETVRRLEKDLEFETN 105
TFIIE-A_C pfam11521
C-terminal general transcription factor TFIIE alpha; TFIIE is compiled of two subunits, alpha ...
 394-439 2.29e-26

C-terminal general transcription factor TFIIE alpha; TFIIE is compiled of two subunits, alpha and beta. This family of proteins are the C terminal domain of the alpha subunit of the protein which is the largest subunit and contains several functional domains which are important for basal transcription and cell growth. The C terminal end of the protein binds directly to the amino-terminal PH domain of p62/Tfb1 (of IIH) which is involved in the recruitment of the general transcription factor IIH to the transcription preinitiation complex. P53 competes for the same binding site as TFIIE alpha which shows their structural similarity. Like p53, TFIIE alpha 336-439 can activate transcription in vivo.


Pssm-ID: 463287  Cd Length: 85  Bit Score: 101.64  E-value: 2.29e-26
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*.
gi 116242812  394 PIVMVAGRPFSYSEVSQRPELVAQMTPEEKEAYIAMGQRMFEDLFE 439
Cdd:pfam11521  40 PTVMVAGRPVSYSEVSQRPELVAQMTPEEKEAYIKVGQQMFSDMYD 85
TF_Zn_Ribbon pfam08271
TFIIB zinc-binding; The transcription factor TFIIB contains a zinc-binding motif near the ...
 127-175 1.39e-06

TFIIB zinc-binding; The transcription factor TFIIB contains a zinc-binding motif near the N-terminus. This domain is involved in the interaction with RNA pol II and TFIIF and plays a crucial role in selecting the transcription initiation site. The domain adopts a zinc ribbon like structure.


Pssm-ID: 400527 [Multi-domain]  Cd Length: 43  Bit Score: 44.61  E-value: 1.39e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 116242812  127 FKCPVCSSTFTdleanqLFDPMTGTFRCTFChTEVEEDESAMPKKDART 175
Cdd:pfam08271   1 YKCPNCGSTNL------VEDYERGELVCTDC-GLVLEDNSIDEGPEWRE 42
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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