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Conserved domains on  [gi|12644253|sp|P39163|]
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RecName: Full=Glutathione-specific gamma-glutamylcyclotransferase; Short=Gamma-GCG; AltName: Full=Cation transport regulatory protein ChaC

Protein Classification

gamma-glutamylcyclotransferase( domain architecture ID 11467097)

gamma-glutamylcyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 32-219 1.72e-96

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


:

Pssm-ID: 442917  Cd Length: 190  Bit Score: 279.40  E-value: 1.72e-96
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253  32 ASLAATLACRPDEGPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGGRTTGVAYRL 111
Cdd:COG3703   1 ASLNRTLAGRPGGGDLWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGLDRGGSCRGVAFRV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253 112 PEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFS 191
Cdd:COG3703  81 PPEDAEAVLAYLWRREMVTGVYLPRWLPVRLADGRRVEALAFVADRDHPQYAGGLSLEEQARIIAQAVGPSGSNADYLLN 160

                       170       180
                ....*....|....*....|....*...
gi 12644253 192 LEQELIKLGMQDDGLNDLLVSVKKLLAE 219
Cdd:COG3703 161 TAEHLRELGIRDPHLEALARLVRALLAA 188
 
Name Accession Description Interval E-value
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 32-219 1.72e-96

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 279.40  E-value: 1.72e-96
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253  32 ASLAATLACRPDEGPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGGRTTGVAYRL 111
Cdd:COG3703   1 ASLNRTLAGRPGGGDLWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGLDRGGSCRGVAFRV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253 112 PEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFS 191
Cdd:COG3703  81 PPEDAEAVLAYLWRREMVTGVYLPRWLPVRLADGRRVEALAFVADRDHPQYAGGLSLEEQARIIAQAVGPSGSNADYLLN 160

                       170       180
                ....*....|....*....|....*...
gi 12644253 192 LEQELIKLGMQDDGLNDLLVSVKKLLAE 219
Cdd:COG3703 161 TAEHLRELGIRDPHLEALARLVRALLAA 188
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 47-216 5.47e-82

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 242.30  E-value: 5.47e-82
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253    47 VWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEG--GRTTGVAYRLPEETLEQELTLLW 124
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDeeGRTWGVAYRIPAETAEEVLEYLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253   125 KREMITG---CYLPTWCQLDlDDGRTVNAIVFIMDPRHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLG- 200
Cdd:pfam04752  81 VREQINGyttHEVPFYPDQD-TDGRPVNALVYVATPKNPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQALRKLGp 159

                         170
                  ....*....|....*..
gi 12644253   201 -MQDDGLNDLLVSVKKL 216
Cdd:pfam04752 160 gVQDDHLFELEKLVKKL 176
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 49-155 4.84e-16

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 70.84  E-value: 4.84e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253  49 IFGYGSLMWNPALE-----FTESCTGTLVGWHRAFCLrltagrgtaHQPGRMLALKEGGRTTGVAYRLPEETLEQeltlL 123
Cdd:cd06661   1 LFVYGTLMDGEVLHarlgrALFLGPATLKGYRLVFGG---------GSGYPGLVPGPGARVWGELYEVDPEDLAR----L 67

                        90       100       110
                ....*....|....*....|....*....|..
gi 12644253 124 WKREMITGCYLPTWCQLDLDDGRTVNAIVFIM 155
Cdd:cd06661  68 DAFEGVPGGYRREEVEVELEDGEGVEAWVYVA 99
 
Name Accession Description Interval E-value
ChaC2 COG3703
Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and ...
 32-219 1.72e-96

Gamma-glutamylcyclotransferase ChaC2 (glutathione degradation) [Inorganic ion transport and metabolism];


Pssm-ID: 442917  Cd Length: 190  Bit Score: 279.40  E-value: 1.72e-96
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253  32 ASLAATLACRPDEGPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGGRTTGVAYRL 111
Cdd:COG3703   1 ASLNRTLAGRPGGGDLWVFGYGSLIWNPGFEFVERRPARLHGWHRSFCLRSTVHRGTPERPGLVLGLDRGGSCRGVAFRV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253 112 PEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFS 191
Cdd:COG3703  81 PPEDAEAVLAYLWRREMVTGVYLPRWLPVRLADGRRVEALAFVADRDHPQYAGGLSLEEQARIIAQAVGPSGSNADYLLN 160

                       170       180
                ....*....|....*....|....*...
gi 12644253 192 LEQELIKLGMQDDGLNDLLVSVKKLLAE 219
Cdd:COG3703 161 TAEHLRELGIRDPHLEALARLVRALLAA 188
ChaC pfam04752
ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases ...
 47-216 5.47e-82

ChaC-like protein; The ChaC family of proteins function as gamma-glutamyl cyclotransferases acting specifically to degrade glutathione but not other gamma-glutamyl peptides. It is is conversed across all phyla and represents a new pathway for glutathione degradation in living cells.


Pssm-ID: 398428  Cd Length: 176  Bit Score: 242.30  E-value: 5.47e-82
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253    47 VWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEG--GRTTGVAYRLPEETLEQELTLLW 124
Cdd:pfam04752   1 LWVFGYGSLIWKPGFPYTDSRTGFIHGYARRFWQGSTDHRGTPERPGRVLTLIEDeeGRTWGVAYRIPAETAEEVLEYLD 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253   125 KREMITG---CYLPTWCQLDlDDGRTVNAIVFIMDPRHPEYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLG- 200
Cdd:pfam04752  81 VREQINGyttHEVPFYPDQD-TDGRPVNALVYVATPKNPQYLGDDPLEQIAKQIATARGPSGPNAEYLFELAQALRKLGp 159

                         170
                  ....*....|....*..
gi 12644253   201 -MQDDGLNDLLVSVKKL 216
Cdd:pfam04752 160 gVQDDHLFELEKLVKKL 176
GGCT_like cd06661
GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) ...
 49-155 4.84e-16

GGCT-like domains, also called AIG2-like family. Gamma-glutamyl cyclotransferase (GGCT) catalyzes the formation of pyroglutamic acid (5-oxoproline) from dipeptides containing gamma-glutamyl, and is a dimeric protein. In Homo sapiens, the protein is encoded by the gene C7orf24, and the enzyme participates in the gamma-glutamyl cycle. Hereditary defects in the gamma-glutamyl cycle have been described for some of the genes involved, but not for C7orf24. The synthesis and metabolism of glutathione (L-gamma-glutamyl-L-cysteinylglycine) ties the gamma-glutamyl cycle to numerous cellular processes; glutathione acts as a ubiquitous reducing agent in reductive mechanisms involved in protein and DNA synthesis, transport processes, enzyme activity, and metabolism. AIG2 (avrRpt2-induced gene) is an Arabidopsis protein that exhibits RPS2- and avrRpt2-dependent induction early after infection with Pseudomonas syringae pv maculicola strain ES4326 carrying avrRpt2. avrRpt2 is an avirulence gene that can convert virulent strains of P. syringae to avirulence on Arabidopsis thaliana, soybean, and bean. The family also includes bacterial tellurite-resistance proteins (trgB); tellurium (Te) compounds are used in industrial processes and had been used as antimicrobial agents in the past. Some members have been described proteins involved in cation transport (chaC).


Pssm-ID: 119400  Cd Length: 99  Bit Score: 70.84  E-value: 4.84e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253  49 IFGYGSLMWNPALE-----FTESCTGTLVGWHRAFCLrltagrgtaHQPGRMLALKEGGRTTGVAYRLPEETLEQeltlL 123
Cdd:cd06661   1 LFVYGTLMDGEVLHarlgrALFLGPATLKGYRLVFGG---------GSGYPGLVPGPGARVWGELYEVDPEDLAR----L 67

                        90       100       110
                ....*....|....*....|....*....|..
gi 12644253 124 WKREMITGCYLPTWCQLDLDDGRTVNAIVFIM 155
Cdd:cd06661  68 DAFEGVPGGYRREEVEVELEDGEGVEAWVYVA 99
GGACT pfam06094
Gamma-glutamyl cyclotransferase, AIG2-like; GGACT, gamma-glutamylamine cyclotransferase, is a ...
 49-162 4.61e-07

Gamma-glutamyl cyclotransferase, AIG2-like; GGACT, gamma-glutamylamine cyclotransferase, is a ubiquitous enzyme found in bacteria, plants, and metazoans from Dictyostelium through to humans. It converts gamma-glutamylamines to free amines and 5-oxoproline.


Pssm-ID: 428763 [Multi-domain]  Cd Length: 118  Bit Score: 47.38  E-value: 4.61e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 12644253    49 IFGYGSLM--------WNPALEFTESCTGTLVGWHRAFClrltagrgtAHQPGrmLALKEGGRTTGVAYRLPEETLEQ-- 118
Cdd:pfam06094   1 VFVYGTLRrgepnhhaLLLGARFPGLGPATLPGYRLYDL---------GGYPA--LVPGEGERVVGELYEVDDALLARld 69

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....
gi 12644253   119 ELtllwkrEMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHPEY 162
Cdd:pfam06094  70 EL------EGYPNLYEREVVEVEVEDGEPVEAWVYVYNPDDEGG 107
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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