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Conserved domains on  [gi|158749611|ref|NP_032847|]
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DDB1- and CUL4-associated factor 8-like protein 2 [Mus musculus]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 345-666 2.91e-26

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 109.35  E-value: 2.91e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 345 LEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWDwLKKRSVLNFDSGHKNNILQAKFLpnCNDAILAMCGRDGQVRV 424
Cdd:cd00200    1 LRRTLKGHTGGVTCVAFSPDGKLLATGSGDGTIKVWD-LETGELLRTLKGHTGPVRDVAAS--ADGTYLASGSSDKTIRL 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 425 AQLSavaGTHMTKRLVKHGGASHRLGLEPDSPFrFLTSGED--AVVFNIDLRQahpasKLLVIKDGDKKVglYTVFVNPA 502
Cdd:cd00200   78 WDLE---TGECVRTLTGHTSYVSSVAFSPDGRI-LSSSSRDktIKVWDVETGK-----CLTTLRGHTDWV--NSVAFSPD 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 503 NvyQFAVGG-QDQFMRIYDqrkidenVNNGVLKKFCPHHllsSDYpahITSLMYSYDGTEILASYNDEDIYIFnssDSDG 581
Cdd:cd00200  147 G--TFVASSsQDGTIKLWD-------LRTGKCVATLTGH---TGE---VNSVAFSPDGEKLLSSSSDGTIKLW---DLST 208

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 582 AQYAKRYKGHRNnstvkGVYF--YGPRSEFVMSGSDCGHIFIWEKSSCQIVQFLEADEGGtINCIDSHPYLPVLASSGLD 659
Cdd:cd00200  209 GKCLGTLRGHEN-----GVNSvaFSPDGYLLASGSEDGTIRVWDLRTGECVQTLSGHTNS-VTSLAWSPDGKRLASGSAD 282


                 ....*..
gi 158749611 660 HEVKIWS 666
Cdd:cd00200  283 GTIRIWD 289
CobT2 super family cl34772
Cobalamin biosynthesis cobaltochelatase CobT subunit [Coenzyme transport and metabolism]; ...
 224-294 4.52e-03

Cobalamin biosynthesis cobaltochelatase CobT subunit [Coenzyme transport and metabolism]; Cobalamin biosynthesis cobaltochelatase CobT subunit is part of the Pathway/BioSystem: Cobalamine/B12 biosynthesis


The actual alignment was detected with superfamily member COG4547:

Pssm-ID: 443611 [Multi-domain]  Cd Length: 608  Bit Score: 40.16  E-value: 4.52e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 158749611 224 REVIGAQGGQGLSRhygLEggEVVE-----ATAVRRLIQHHELEEGEDVDDQEESSEMHEETSEDSSEQYDIEDDS 294
Cdd:COG4547  170 RDWIEEKAGADLDR---LA--ELLDdqaafARAVRDLLRDLDLAEELGEDEDEEDEDDEDDSGEQEEDEEDGEDED 240
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 345-666 2.91e-26

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 109.35  E-value: 2.91e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 345 LEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWDwLKKRSVLNFDSGHKNNILQAKFLpnCNDAILAMCGRDGQVRV 424
Cdd:cd00200    1 LRRTLKGHTGGVTCVAFSPDGKLLATGSGDGTIKVWD-LETGELLRTLKGHTGPVRDVAAS--ADGTYLASGSSDKTIRL 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 425 AQLSavaGTHMTKRLVKHGGASHRLGLEPDSPFrFLTSGED--AVVFNIDLRQahpasKLLVIKDGDKKVglYTVFVNPA 502
Cdd:cd00200   78 WDLE---TGECVRTLTGHTSYVSSVAFSPDGRI-LSSSSRDktIKVWDVETGK-----CLTTLRGHTDWV--NSVAFSPD 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 503 NvyQFAVGG-QDQFMRIYDqrkidenVNNGVLKKFCPHHllsSDYpahITSLMYSYDGTEILASYNDEDIYIFnssDSDG 581
Cdd:cd00200  147 G--TFVASSsQDGTIKLWD-------LRTGKCVATLTGH---TGE---VNSVAFSPDGEKLLSSSSDGTIKLW---DLST 208

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 582 AQYAKRYKGHRNnstvkGVYF--YGPRSEFVMSGSDCGHIFIWEKSSCQIVQFLEADEGGtINCIDSHPYLPVLASSGLD 659
Cdd:cd00200  209 GKCLGTLRGHEN-----GVNSvaFSPDGYLLASGSEDGTIRVWDLRTGECVQTLSGHTNS-VTSLAWSPDGKRLASGSAD 282


                 ....*..
gi 158749611 660 HEVKIWS 666
Cdd:cd00200  283 GTIRIWD 289
WD40 COG2319
WD40 repeat [General function prediction only];
345-667 7.19e-20

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 92.67  E-value: 7.19e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 345 LEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWDWLKKRSVLNFDsGHKNNILQAKFLPncNDAILAMCGRDGQVRV 424
Cdd:COG2319  154 LLRTLTGHSGAVTSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLT-GHTGAVRSVAFSP--DGKLLASGSADGTVRL 230

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 425 AQlsaVAGTHMTKRLVKHGGASHrlglepdspfrfltsgedAVVFnidlrqaHPASKLLvikdgdkkvglytvfvnpanv 504
Cdd:COG2319  231 WD---LATGKLLRTLTGHSGSVR------------------SVAF-------SPDGRLL--------------------- 261

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 505 yqfAVGGQDQFMRIYDqrkidenVNNGVLKKFCPHHllssdyPAHITSLMYSYDGTEILASYNDEDIYIFnssDSDGAQY 584
Cdd:COG2319  262 ---ASGSADGTVRLWD-------LATGELLRTLTGH------SGGVNSVAFSPDGKLLASGSDDGTVRLW---DLATGKL 322

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 585 AKRYKGHRNnsTVKGVYFyGPRSEFVMSGSDCGHIFIWEKSSCQIVQFLEADEGGtINCIDSHPYLPVLASSGLDHEVKI 664
Cdd:COG2319  323 LRTLTGHTG--AVRSVAF-SPDGKTLASGSDDGTVRLWDLATGELLRTLTGHTGA-VTSVAFSPDGRTLASGSADGTVRL 398


                 ...
gi 158749611 665 WSP 667
Cdd:COG2319  399 WDL 401
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 343-381 2.58e-08

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 50.39  E-value: 2.58e-08
                           10        20        30
                   ....*....|....*....|....*....|....*....
gi 158749611   343 FSLEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWD 381
Cdd:smart00320   2 GELLKTLKGHTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
343-381 1.10e-07

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 48.50  E-value: 1.10e-07
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 158749611  343 FSLEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWD 381
Cdd:pfam00400   1 GKLLKTLEGHTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
CobT2 COG4547
Cobalamin biosynthesis cobaltochelatase CobT subunit [Coenzyme transport and metabolism]; ...
 224-294 4.52e-03

Cobalamin biosynthesis cobaltochelatase CobT subunit [Coenzyme transport and metabolism]; Cobalamin biosynthesis cobaltochelatase CobT subunit is part of the Pathway/BioSystem: Cobalamine/B12 biosynthesis


Pssm-ID: 443611 [Multi-domain]  Cd Length: 608  Bit Score: 40.16  E-value: 4.52e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 158749611 224 REVIGAQGGQGLSRhygLEggEVVE-----ATAVRRLIQHHELEEGEDVDDQEESSEMHEETSEDSSEQYDIEDDS 294
Cdd:COG4547  170 RDWIEEKAGADLDR---LA--ELLDdqaafARAVRDLLRDLDLAEELGEDEDEEDEDDEDDSGEQEEDEEDGEDED 240
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 345-666 2.91e-26

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 109.35  E-value: 2.91e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 345 LEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWDwLKKRSVLNFDSGHKNNILQAKFLpnCNDAILAMCGRDGQVRV 424
Cdd:cd00200    1 LRRTLKGHTGGVTCVAFSPDGKLLATGSGDGTIKVWD-LETGELLRTLKGHTGPVRDVAAS--ADGTYLASGSSDKTIRL 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 425 AQLSavaGTHMTKRLVKHGGASHRLGLEPDSPFrFLTSGED--AVVFNIDLRQahpasKLLVIKDGDKKVglYTVFVNPA 502
Cdd:cd00200   78 WDLE---TGECVRTLTGHTSYVSSVAFSPDGRI-LSSSSRDktIKVWDVETGK-----CLTTLRGHTDWV--NSVAFSPD 146

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 503 NvyQFAVGG-QDQFMRIYDqrkidenVNNGVLKKFCPHHllsSDYpahITSLMYSYDGTEILASYNDEDIYIFnssDSDG 581
Cdd:cd00200  147 G--TFVASSsQDGTIKLWD-------LRTGKCVATLTGH---TGE---VNSVAFSPDGEKLLSSSSDGTIKLW---DLST 208

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 582 AQYAKRYKGHRNnstvkGVYF--YGPRSEFVMSGSDCGHIFIWEKSSCQIVQFLEADEGGtINCIDSHPYLPVLASSGLD 659
Cdd:cd00200  209 GKCLGTLRGHEN-----GVNSvaFSPDGYLLASGSEDGTIRVWDLRTGECVQTLSGHTNS-VTSLAWSPDGKRLASGSAD 282


                 ....*..
gi 158749611 660 HEVKIWS 666
Cdd:cd00200  283 GTIRIWD 289
WD40 COG2319
WD40 repeat [General function prediction only];
345-667 7.19e-20

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 92.67  E-value: 7.19e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 345 LEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWDWLKKRSVLNFDsGHKNNILQAKFLPncNDAILAMCGRDGQVRV 424
Cdd:COG2319  154 LLRTLTGHSGAVTSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLT-GHTGAVRSVAFSP--DGKLLASGSADGTVRL 230

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 425 AQlsaVAGTHMTKRLVKHGGASHrlglepdspfrfltsgedAVVFnidlrqaHPASKLLvikdgdkkvglytvfvnpanv 504
Cdd:COG2319  231 WD---LATGKLLRTLTGHSGSVR------------------SVAF-------SPDGRLL--------------------- 261

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 505 yqfAVGGQDQFMRIYDqrkidenVNNGVLKKFCPHHllssdyPAHITSLMYSYDGTEILASYNDEDIYIFnssDSDGAQY 584
Cdd:COG2319  262 ---ASGSADGTVRLWD-------LATGELLRTLTGH------SGGVNSVAFSPDGKLLASGSDDGTVRLW---DLATGKL 322

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 585 AKRYKGHRNnsTVKGVYFyGPRSEFVMSGSDCGHIFIWEKSSCQIVQFLEADEGGtINCIDSHPYLPVLASSGLDHEVKI 664
Cdd:COG2319  323 LRTLTGHTG--AVRSVAF-SPDGKTLASGSDDGTVRLWDLATGELLRTLTGHTGA-VTSVAFSPDGRTLASGSADGTVRL 398


                 ...
gi 158749611 665 WSP 667
Cdd:COG2319  399 WDL 401
WD40 COG2319
WD40 repeat [General function prediction only];
336-575 2.30e-12

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 69.55  E-value: 2.30e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 336 ARLF-VQRFSLEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWDwLKKRSVLNFDSGHKNNILQAKFLPncNDAILA 414
Cdd:COG2319  186 VRLWdLATGKLLRTLTGHTGAVRSVAFSPDGKLLASGSADGTVRLWD-LATGKLLRTLTGHSGSVRSVAFSP--DGRLLA 262

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 415 MCGRDGQVRVAQlsaVAGTHMTKRLVKHGGASHRLGLEPDSPfRFLTSGEDAVV--FNIDLRQAhpaskLLVIKDGDKKV 492
Cdd:COG2319  263 SGSADGTVRLWD---LATGELLRTLTGHSGGVNSVAFSPDGK-LLASGSDDGTVrlWDLATGKL-----LRTLTGHTGAV 333

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 493 glYTVFVNPANVyQFAVGGQDQFMRIYDqrkidenVNNGVLKKFCPHHllssdyPAHITSLMYSYDGTEILASYNDEDIY 572
Cdd:COG2319  334 --RSVAFSPDGK-TLASGSDDGTVRLWD-------LATGELLRTLTGH------TGAVTSVAFSPDGRTLASGSADGTVR 397


                 ...
gi 158749611 573 IFN 575
Cdd:COG2319  398 LWD 400
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 340-520 3.00e-11

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 65.05  E-value: 3.00e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 340 VQRFSLEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWDWLKKRSVLNFdSGHKNNILQAKFLPNCNDaiLAMCGRD 419
Cdd:cd00200  122 VETGKCLTTLRGHTDWVNSVAFSPDGTFVASSSQDGTIKLWDLRTGKCVATL-TGHTGEVNSVAFSPDGEK--LLSSSSD 198

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 420 GQVRVAQLSAvagTHMTKRLVKHGGASHRLGLEPDSpfRFLTSG-ED--AVVFNIDLRQahpasKLLVIKDGDKKVglYT 496
Cdd:cd00200  199 GTIKLWDLST---GKCLGTLRGHENGVNSVAFSPDG--YLLASGsEDgtIRVWDLRTGE-----CVQTLSGHTNSV--TS 266

                        170       180
                 ....*....|....*....|....
gi 158749611 497 VFVNPANVYqFAVGGQDQFMRIYD 520
Cdd:cd00200  267 LAWSPDGKR-LASGSADGTIRIWD 289
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 343-381 2.58e-08

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 50.39  E-value: 2.58e-08
                           10        20        30
                   ....*....|....*....|....*....|....*....
gi 158749611   343 FSLEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWD 381
Cdd:smart00320   2 GELLKTLKGHTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
343-381 1.10e-07

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 48.50  E-value: 1.10e-07
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 158749611  343 FSLEHVFEGHSGCVNTVHFNQHGTLLASGSDDLKVIVWD 381
Cdd:pfam00400   1 GKLLKTLEGHTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 507-667 5.52e-07

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 51.95  E-value: 5.52e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 507 FAVGGQDQFMRIYDqrkidenVNNGVLKKFCPHHLLSsdypahITSLMYSYDGTEILASYNDEDIYIFNSSDSDGAQyak 586
Cdd:cd00200   24 LATGSGDGTIKVWD-------LETGELLRTLKGHTGP------VRDVAASADGTYLASGSSDKTIRLWDLETGECVR--- 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 158749611 587 RYKGHrnNSTVKGVYFYgPRSEFVMSGSDCGHIFIWEKSSCQIVQFLEADEGgTINCIDSHPYLPVLASSGLDHEVKIWS 666
Cdd:cd00200   88 TLTGH--TSYVSSVAFS-PDGRILSSSSRDKTIKVWDVETGKCLTTLRGHTD-WVNSVAFSPDGTFVASSSQDGTIKLWD 163


                 .
gi 158749611 667 P 667
Cdd:cd00200  164 L 164
CobT2 COG4547
Cobalamin biosynthesis cobaltochelatase CobT subunit [Coenzyme transport and metabolism]; ...
 224-294 4.52e-03

Cobalamin biosynthesis cobaltochelatase CobT subunit [Coenzyme transport and metabolism]; Cobalamin biosynthesis cobaltochelatase CobT subunit is part of the Pathway/BioSystem: Cobalamine/B12 biosynthesis


Pssm-ID: 443611 [Multi-domain]  Cd Length: 608  Bit Score: 40.16  E-value: 4.52e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 158749611 224 REVIGAQGGQGLSRhygLEggEVVE-----ATAVRRLIQHHELEEGEDVDDQEESSEMHEETSEDSSEQYDIEDDS 294
Cdd:COG4547  170 RDWIEEKAGADLDR---LA--ELLDdqaafARAVRDLLRDLDLAEELGEDEDEEDEDDEDDSGEQEEDEEDGEDED 240
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 626-666 9.70e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.60  E-value: 9.70e-03
                           10        20        30        40
                   ....*....|....*....|....*....|....*....|.
gi 158749611   626 SCQIVQFLEADEGGtINCIDSHPYLPVLASSGLDHEVKIWS 666
Cdd:smart00320   1 SGELLKTLKGHTGP-VTSVAFSPDGKYLASGSDDGTIKLWD 40
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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